Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells

Farea, Manal; Husein, Adam; Halim, Ahmad Sukari; Berahim, Zurairah; Nurul, Asma Abdullah; Mokhtar, Khairani Idah; Mokhtar, Kasmawati

doi:10.1007/s00784-015-1601-6

Cited by 9 publications

(10 citation statements)

References 57 publications

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“…Considering that the cementoblastoma arises from the uncontrolled proliferation of the cementoid matrix by cementoblasts[ 16 ] and that these cells are not present in the middle and cervical portions of the root[ 17 ], in the second surgical approach, removal of the root was performed up to the middle third, thereby eliminating all the cellular cement that could have led to the origin of the second lesion. However, this procedure may not eliminate the possibility of the lesion recurrence since other ethological factors as the uncontrolled induction of the cementoblasts differentiation by the epithelial rests of Malassez cells can be the trigger of the cementoblastoma lesions[ 18 ]. After one year of follow-up of this surgical procedure, repair with bone neoformation was verified in the region of the lesion.…”

Section: Discussionmentioning

confidence: 99%

Surgical treatment of cementoblastoma associated with apicoectomy and endodontic therapy: Case report

Costa¹,

Oliveira²,

Chaves³

et al. 2016

WJCC

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This case report describes the surgical removal of cementoblastoma associated with apicoectomy and endodontic therapy. The patient, an 18-year-old man, presented pain in the region of the mandibular body on the right side. On clinical exam, bone expansion was observed in the region at the bottom of the vestibular sulcus, pain on palpation, slight extrusion of tooth 46 with presence of pulp vitality. Radiographic exams demonstrated the presence of a radiopaque area and discrete radiolucent halo associated with the root of tooth 46, suggesting the diagnosis of cementoblastoma. Endodontic treatment of tooth 46 was performed and exeresis of the lesion by apicoectomy. Twelve months after the first surgery, recurrence of the lesion was observed, and a new apicoectomy was necessary, this time up to the middle third of the root. Clinical radiographic control 12 mo after the second surgical intervention demonstrated absence of signs and symptoms, radiographic repair, with tooth 46 shown to be fully functional.

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Section: Discussionmentioning

confidence: 99%

Surgical treatment of cementoblastoma associated with apicoectomy and endodontic therapy: Case report

Costa¹,

Oliveira²,

Chaves³

et al. 2016

WJCC

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“…The coculture systems were established through 6-well plate and 3 μm pore size transwell inserts (Corning) 21 . The hDPSCs were seeded in 6-well plate at the amount of 5×10 ^4 cells/well and the same quantity of hUCMSCs were seeded in the transwell inserts located in neighboring wells.…”

Section: Methodsmentioning

confidence: 99%

“…For example, UCMSCs could inhibit growth and promote apoptosis of HepG2 hepatocellular carcinoma cells 15 ; the viability and the secretion function were significantly increased in damaged mouse hepatocytes when they were indirect cocultured with UCMSCs 16 ; UCMSCs could efficiently suppress the proliferation and cytotoxicity of T cells and B cells in vitro 17 , 18 . As to DPSCs, some researches proved that they could affect biological characters of lymphocytes 19 , 20 and Malassez cells 21 in coculture condition. In recent years, a few scholars found that the paracrine effects also existed among different kinds of stem cells, which meant that the proliferation or differentiation abilities of stem cells could be modified by other kinds of stem cells 22 - 26 .…”

Section: Introductionmentioning

confidence: 99%

The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System

Jia

Zhang

et al. 2017

Int. J. Med. Sci.

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Objectives: The present study established a non-contact coculture system in vitro, aiming to investigate the crosstalk between human dental pulp stem cells (hDPSCs) and human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation activity and osteogenic genes expression through paracrine.Materials and methods: The stemness of hDPSCs and hUCMSCs were identified by flow cytometric analysis and multipotential differentiation assays. With the help of transwell inserts, the non-contact coculture system in vitro was established between hDPSCs and hUCMSCs. EdU labeling analysis and Western Blot were used to detect the proliferation activity. The mRNA and protein levels of osteogenic genes were evaluated by RT-PCR and Western Blot. The expression of elements in Akt/mTOR signaling pathway were detected by Western Blot.Results: Both hDPSCs and hUCMSCs were positive to MSCs specific surface markers and had multi-differentiation potential. The proportion of EdU-positive cells increased and the expression of CDK6 and CYCLIN A were up-regulated in cocultured hDPSCs. Both prior coculture and persistent coculture improved mRNA and protein levels of osteogenic genes in hDPSCs. While in cocultured hUCMSCs, no statistical differences were observed on proliferation and osteogenesis. The phosphorylation of Akt and mTOR was up-regulated in cocultured hDPSCs.Conclusions: The crosstalk between hDPSCs and hUCMSCs in coculture system increased the proliferation activity and enhanced osteogenic genes expression in hDPSCs. Akt/mTOR signaling pathway might take part in the enhancing effects in both cell proliferation and gene expression.

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“…17 TGF-β1 signalling from HERS is responsible for inducing cementoblast differentiation. 18 Some authors have postulated a different origin of cementoblasts where HERS cells themselves undergo epithelial-ectomesenchymal transformation and directly differentiate into cementoblasts. 9,16,19 TGF-β1 and FGF2 have been proposed as factors stimulating this epithelial-ectomesenchymal transformation of HERS cells through a MAPK/ERK-dependent signalling pathway.…”

Section: Review Of Tooth Root Developmentmentioning

confidence: 99%

Fragmentary tooth root development: biological and forensic dental implications

Nel¹,

Davidson²,

Uys³

et al. 2017

S. Afr. dent. j.

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Recent findings indicate that there could be continued root development after the successful surgical removal of an impacted tooth. The paper provides a brief review of normal root development, emphasizing the chain of reciprocal epithelial-ectomesenchymal interactions which regulate all aspects of this process. Mineralized dental structures are not an absolute requirement for tooth root development, but residual fragments of Hertwig's epithelial root sheath (HERS), together with the associated ectomesenchymal cells, will enable continued growth. The findings presented in this paper have significant implications in forensic odontology, dental litigation and for routine and elective tooth extractions.

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Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells

Abstract: The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.

Cited by 9 publications

References 57 publications

Surgical treatment of cementoblastoma associated with apicoectomy and endodontic therapy: Case report

Surgical treatment of cementoblastoma associated with apicoectomy and endodontic therapy: Case report

The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System

Fragmentary tooth root development: biological and forensic dental implications

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