Centella asiatica ameliorates diabetes-induced stress in rat tissues via influences on antioxidants and inflammatory cytokines

Masola, Bubuya; Oguntibeju, Oluwafemi Omoniyi; Oyenihi, Ayodeji B.

doi:10.1016/j.biopha.2018.02.115

Cited by 56 publications

(33 citation statements)

References 39 publications

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“…A significant increase in the mean area of pale nuclei versus diabetic and BMMSCs groups was reported. These results indicated regression of degenerative changes and obvious regeneration versus diabetic and BMMSCs groups, that can be referred to AA antioxidant effect, in agreement with Masola et al (5).…”

Section: Discussionsupporting

confidence: 91%

“…In diabetes, accumulation of reactive oxygen species (ROS) interferes with normal tissue and organ function. Antioxidants as ascorbic acid (AA) may shift the balance back to antioxidant defense that may protect diabetic tissues from stress by affecting oxidant and antioxidant biomarkers that can be useful in the management of diabetic complications (5). Oyarce et al (6) assumed induction of SCs differentiation by AA.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Stem Cells, Ascorbic Acid and SERCA1a Gene Transfected Stem Cells in Experimentally Induced Type I Diabetic Myopathy

Zickri

Sadek

Fares

et al. 2020

IJSC

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Background and Objectives: Sarco/endoplasmic reticulum Ca 2＋ -ATPase (SERCA) inhibition was proved in streptozotocin (STZ)-diabetic rats. The present study aimed at investigating and comparing the therapeutic effect of bone marrow mesenchymal stem cells (BMMSCs), BMMSCs combined with ascorbic acid (AA) and SERCA1a gene transfected BMMSCs in induced type I diabetic myopathy of male albino rat. Methods and Results: 54 rats were divided into donor group of 6 rats for isolation, propagation and characterization of BMMSCs and SERCA1a transfected BMMSCs, groups I∼V 48 rats. Group I of 8 control rats, group II (Diabetic) of 10 rats given STZ 50 mg/kg intraperitoneal, group III (BMMSCs) of 10 rats given STZ and BMMSCs intravenous (IV), group IV (BMMSCs and AA) of 10 rats given STZ, BMMSCs IV and AA 500 mg/kg and group V (SERCA 1a transfected BMMSCs) of 10 rats given STZ and SERCA1a transfected BMMSCs IV. The rats were sacrificed after 8 weeks. Gastrocnemius specimens were subjected to biochemical, histological, morphometric and statistical studies. Diabetic rats revealed inflammatory and degenerative muscle changes, a significant increase in blood glucose level, mean DNA fragmentation and mean MDA values and a significant decrease in mean GSH and catalase values, area of pale nuclei, area% of CD105 and CD34 ＋ve cells, SERCA1a protein and gene values. The morphological changes regressed by therapy. In group III significant decrease in DNA fragmentation and MDA, significant increase in GSH and catalase, significant increase in the mean area of pale nuclei, area % of CD105 and CD34 ＋ve cells versus diabetic group. In group IV, same findings as group III versus diabetic and BMMSCs groups. In group V, same findings as group IV versus diabetic and treated groups. Western blot and PCR proved a mean value of SERCA1a protein and gene comparable to the control group. Mean calcium concentration values revealed a significant increase in the diabetic group, in BMMSCs and AA group versus control and SERCA1a group. Conclusions: SERCA1a transfected BMMSCs proved a definite therapeutic effect, more remarkable than BMMSCs combined with AA. This effect was evidenced histologically and confirmed by significant changes in the biochemical tests indicating oxidative stress, muscle calcium concentration, morphometric parameters and PCR values of SERCA1a.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Effect of Stem Cells, Ascorbic Acid and SERCA1a Gene Transfected Stem Cells in Experimentally Induced Type I Diabetic Myopathy

Zickri

Sadek

Fares

et al. 2020

IJSC

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“…Briefly, a 10% fructose solution was provided as drinking water ad libitum for rats for 14 days followed by a single intraperitoneal injection of low-dose streptozotocin (STZ; 40 mg/kg body weight in 0.1 M citrate buffer (pH 4.5)). Diabetes induction was confirmed by polyphagia, polydipsia and high blood glucose values of at least 12 mM measured a week after the STZ injection as we have reported previously (Masola et al, 2018;Oyenihi et al, 2019). Nondiabetic control rats were given drinking water ad libitum for 14 days followed by a single intraperitoneal injection of 0.1 M citrate buffer (pH 4.5).…”

Section: Ethical Handling Of Animals and Induction Of Diabetesmentioning

confidence: 86%

Centella asiaticaalleviates diabetes‐induced changes in fatty acid profile and oxidative damage in rat testis

et al. 2020

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The in vivo effects of Centella asiatica L. Urban (Family: Apiaceae; CA) on diabetesinduced testicular fatty acid misdistribution and oxidative injury were investigated. Diabetic rats were treated with vehicle, CA or metformin daily for 14 days by oral gavage. Fatty acid (FA) content in testis was analysed using gas chromatographyflame ionisation detection while redox indices were measured as peroxide value (PV), malondialdehyde (MDA), oxygen radical antioxidant capacity (ORAC), reduced glutathione (GSH), glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities. Diabetes increased omega-6 (61%), and decreased omega-3 (23%) and monounsaturated fatty acids (MUFA; 18%) compared to non-diabetic controls. Oxidative injury in diabetic rats was confirmed by increases in PV (112%) and MDA (77%) in addition to decreases in GSH (41%) and activities of GST (19%) & GPx (24%) compared to non-diabetic controls. CA treatment led to 17% reduction in omega-6 and 33% rise in MUFA compared to diabetic controls. Additionally, CA ameliorated the oxidative injury and improved antioxidant capacity by increasing GSH (49%), GST (16%) and GPx (23%) when compared to diabetic controls. Data suggest CA potential in alleviating the alterations caused by diabetes in testes through effects on omega-6 and MUFA; and via increased GSH level and dependent enzyme activities.

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“…The flavonoids content of N. tabacum could be responsible for the activities of the plant extract. This is because flavonoids possess antioxidant and antidiabetic potentials consequent to the existence of hydroxyl groups, thus acting as powerful scavengers of ROS, thereby playing a significant role in preventing lipid peroxidation and preserving betacell function and cellular integrity [5,25,[30][31][32].…”

Section: Oguntibejumentioning

confidence: 99%

“…Honestly, the use of medicinal plants is gaining interest not just across Africa and Asia but even in developed countries such as America and Europe [1,2]. The increase in the use of medicinal plants in developing countries is prompted by poverty, high cost of orthodox drugs, availability of medicinal plants coupled with adequate knowledge of the local community, effectiveness of medicinal plants in the treatment of certain conditions, and availability of scientific findings that support the use of medicinal plants and the perception of the low side effects as well as the improved quality in the preparation of medicinal products in developing countries [3][4][5]. Medicinal plants continue to play an important role in the treatment of various diseases and one of such plants is Nicotiana tabacum that has gained global attention and application as therapeutic agent.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic and Biological Activities of Nicotiana Tabacum: Possible Mechanisms

Oguntibeju

2020

Asian J Pharm Clin Res

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Diabetes mellitus is a growing global health problem. Reports predicted that diabetes mellitus could reach a pandemic level affecting people in both developing and developed countries. According to the World Health Organization, high blood glucose is seen as the third highest risk factor for premature mortality in diabetics. For several thousands of years before the adventure of orthodox medications, human beings have been using medicinal plants in the treatment of various disease conditions such as cancer, cardiovascular disease, infertility, bacterial, viral infections, and diabetes mellitus. The beneficial effects of selected medicinal therapies in the treatment and management of diabetes and diabetic complications have been reported. Many medicinal plants across Africa and Asia are known for their rich phytochemicals with effective components that have been observed to effectively reduce high blood glucose either in diabetic persons or diabetes-induced animals. The motivation for the use of medicinal plants includes availability, affordability, knowledge, effectiveness, and safety and some are of the opinion that medicinal plants and their derivatives could be effective and safe alternative treatment for diabetes and diabetic complications. Nicotiana tabacum is one of those plants with antidiabetic and pharmacological potentials which could benefit humans and animals if appropriately utilized; however, less attention has been focused on its antidiabetic properties. In fact, there is a paucity of information on the antidiabetic activities of N. tabacum. Therefore, this paper examines the antidiabetic and other biological activities of N. tabacum.

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Centella asiatica ameliorates diabetes-induced stress in rat tissues via influences on antioxidants and inflammatory cytokines

Cited by 56 publications

References 39 publications

Effect of Stem Cells, Ascorbic Acid and SERCA1a Gene Transfected Stem Cells in Experimentally Induced Type I Diabetic Myopathy

Effect of Stem Cells, Ascorbic Acid and SERCA1a Gene Transfected Stem Cells in Experimentally Induced Type I Diabetic Myopathy

Centella asiaticaalleviates diabetes‐induced changes in fatty acid profile and oxidative damage in rat testis

Antidiabetic and Biological Activities of Nicotiana Tabacum: Possible Mechanisms

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