Centhaquin improves survival in a swine model of hemorrhagic shock

Papapanagiotou, Panagiotis; Xanthos, Theodoros; Gulati, Anil; Chalkias, Athanasios; Papalois, Αpostolos; Kontouli, Z; Alegakis, Athanasios; Iacovidou, Nicoletta

doi:10.1016/j.jss.2015.06.056

Cited by 15 publications

(20 citation statements)

References 41 publications

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“…One week prior to the experiments, the animals were transported to the research facility (Experimental-Research Center Elpen, European Ref Number EL 09 BIO 03) and were acclimatized to laboratory conditions, as previously described [9][10][11]. The day before the experimentation, the animals were fasted but had free access to water.…”

Section: Origin and Source Of The Animalsmentioning

confidence: 99%

“…For measurement of aortic pressures, an arterial catheter (model 6523, USCI CR, Bart; Papapostolou, Athens, Greece) was inserted and forwarded into the descending aorta after surgical preparation of the right internal carotid artery. The systolic (SAP) and diastolic (DAP) arterial pressures were recorded, and mean arterial pressure (MAP) was determined by electronic integration of the aortic blood pressure waveform [11]. The left internal jugular vein was also cannulated, and a Swan-Ganz catheter (Opticath 5.5F, 75 cm; Abbott, Ladakis, Athens, Greece) was inserted into the right atrium.…”

Section: Animal Preparationmentioning

confidence: 99%

“…CO was measured as the product of time-velocity integral of Doppler transaortic flow, the diameter of the aortic valve, and heart rate; SVR was calculated using the formula SVR=(MAP-CVP)/CO×80, as previously described [10,11]. Coronary perfusion pressure (CPP) was electronically calculated as the difference between minimal DAP and the simultaneously measured right atrial diastolic pressure [12].…”

Section: Animal Preparationmentioning

confidence: 99%

“…The protocol simulated a major hemorrhage scenario and has been previously described [11]. In brief, the protocol was divided into four distinct phases: stabilization, hemorrhagic, cardiac arrest, and resuscitation phases ( Figure 1).…”

Section: Experimental Protocolmentioning

confidence: 99%

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Measurement of mean systemic filling pressure after severe hemorrhagic shock in swine anesthetized with propofol-based total intravenous anesthesia: implications for vasopressor-free resuscitation

et al. 2020

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Background:Mean systemic filling pressure (Pmsf) is a quantitative measurement of a patient's volume status and represents the tone of the venous reservoir. The aim of this study was to estimate Pmsf after severe hemorrhagic shock and cardiac arrest in swine anesthetized with propofol-based total intravenous anesthesia, as well as to evaluate Pmsf's association with vasopressor-free resuscitation. Methods: Ten healthy Landrace/Large-White piglets aged 10-12 weeks with average weight 20 ± 1 kg were used in this study. The protocol was divided into four distinct phases: stabilization, hemorrhagic, cardiac arrest, and resuscitation phases. We measured Pmsf at 5-7.5 seconds after the onset of cardiac arrest and then every 10 seconds until 1 minute postcardiac arrest. During resuscitation, lactated Ringers was infused at a rate that aimed for a mean right atrial pressure of ≤ 4 mm Hg. No vasopressors were used. Results: The mean volume of blood removed was 860 ± 20 ml (blood loss, ~61%) and the bleeding time was 43.2 ± 2 minutes while all animals developed pulseless electrical activity. Mean Pmsf was 4.09 ± 1.22 mm Hg, and no significant differences in Pmsf were found until 1 minute postcardiac arrest (4.20±0.22 mm Hg at 5-7.5 seconds and 3.72±0.23 mm Hg at 55-57.5 seconds; P=0.102). All animals achieved return of spontaneous circulation (ROSC), with mean time to ROSC being 6.1 ±1.7 minutes and mean administered volume being 394 ±20 ml. Conclusions: For the first time, Pmsf was estimated after severe hemorrhagic shock. In this study, Pmsf remained stable during the first minute post-arrest. All animals achieved ROSC with goal-directed fluid resuscitation and no vasopressors.

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Section: Origin and Source Of The Animalsmentioning

confidence: 99%

Section: Animal Preparationmentioning

confidence: 99%

Section: Animal Preparationmentioning

confidence: 99%

Section: Experimental Protocolmentioning

confidence: 99%

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Measurement of mean systemic filling pressure after severe hemorrhagic shock in swine anesthetized with propofol-based total intravenous anesthesia: implications for vasopressor-free resuscitation

et al. 2020

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“…We established that low doses of centhaquin (2-[2-(4-(3-methyphenyl)-1-piperazinyl)]ethyl-quinoline) citrate, significantly decreased blood lactate, and increased mean arterial pressure (MAP), pulse pressure (PP) and cardiac output (CO) in hemorrhagic shock (Gulati et al 2012, Papapanagiotou et al 2016. We further carried out comparative studies between centhaquin and status quo resuscitative agents grouped into 3 different categories: a) fluids such as Lactated Ringer's, hypertonic saline; b) adrenergic agents such as norepinephrine, and c) fresh blood.…”

Section: Introductionmentioning

confidence: 99%

Alterations in Endothelin Receptors Following Hemorrhage and Resuscitation by Centhaquin

et al. 2018

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Endothelin-1 (ET-1) acts on ETA and ETB receptors and has been implicated in hemorrhagic shock (shock). We determined effect of shock and resuscitation by hypertonic saline (saline) or centhaquin on ETA and ETB receptor expression. Rats were anesthetized, a pressure catheter was placed in the left femoral artery; blood was withdrawn from the right femoral artery to bring mean arterial pressure (MAP) to 35 mm Hg for 30 min, resuscitation was performed and 90 min later sacrificed to collect samples for biochemical estimations. Resuscitation with centhaquin decreased blood lactate and increased MAP. Protein levels of ETA or ETB receptor were unaltered in the brain, heart, lung and liver following shock or resuscitation. In the abdominal aorta, shock produced an increase (140 %) in ETA expression which was attenuated by saline and centhaquin; ETB expression was unaltered following shock but was increased (79 %) by centhaquin. In renal medulla, ETA expression was unaltered following shock, but was decreased (-61 %) by centhaquin; shock produced a decrease (-34 %) in ETB expression which was completely attenuated by centhaquin and not saline. Shock induced changes in ETA and ETB receptors in the aorta and renal medulla are reversed by centhaquin and may be contributing to its efficacy.

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Resuscitation with centhaquin and 6% hydroxyethyl starch 130/0.4 improves survival in a swine model of hemorrhagic shock: a randomized experimental study

Kontouli

Staikou

Iacovidou

et al. 2018

Eur J Trauma Emerg Surg

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Centhaquin improves survival in a swine model of hemorrhagic shock

Cited by 15 publications

References 41 publications

Measurement of mean systemic filling pressure after severe hemorrhagic shock in swine anesthetized with propofol-based total intravenous anesthesia: implications for vasopressor-free resuscitation

Measurement of mean systemic filling pressure after severe hemorrhagic shock in swine anesthetized with propofol-based total intravenous anesthesia: implications for vasopressor-free resuscitation

Alterations in Endothelin Receptors Following Hemorrhage and Resuscitation by Centhaquin

Resuscitation with centhaquin and 6% hydroxyethyl starch 130/0.4 improves survival in a swine model of hemorrhagic shock: a randomized experimental study

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