Central administration of insulin and refeeding lead to Akt and ERK phosphorylation in the chicken medulla

Saneyasu, Takaoki; Ueno, Mizuki; Nagata, Kanami; Kewan, Ahmed; Honda, Kazuhisa; Kamisoyama, Hiroshi

doi:10.1016/j.neulet.2021.136008

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…Furthermore, the ICV injection of insulin increases POMC mRNA levels in the hypothalamus of layer chicks, but not in broiler chicks[ 87 ]. ICV injection of insulin and refeeding lead to the phosphorylation of both Akt and ERK in the medulla oblongata of layer chicks[ 88 ], and the phosphorylation of Akt, but not ERK, in broiler chicks (unpublished data). These differences between broiler and layer chicks may be one of the causes of the differences in food intake.…”

Section: Cellular Signaling Pathwaysmentioning

confidence: 99%

Recent Research on Mechanisms of Feeding Regulation in Chicks

Saneyasu

2024

J. Poult. Sci.

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Food intake affects poultry productivity. A complete understanding of these regulatory mechanisms provides new strategies to improve productivity. Food intake is regulated by complex mechanisms involving many factors, including the central nervous system, gastrointestinal tract, hormones, and nutrients. Although several studies have been conducted to elucidate regulatory mechanisms in chickens, the mechanisms remain unclear. To update the current knowledge on feeding regulation in chickens, this review focuses on recent findings that have not been summarized in previous reviews, including spexins, adipokines, neurosecretory proteins GL and GM, and central intracellular signaling factors.

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Section: Cellular Signaling Pathwaysmentioning

confidence: 99%

Recent Research on Mechanisms of Feeding Regulation in Chicks

Saneyasu

2024

J. Poult. Sci.

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“…Any abnormalities will affect cell growth, differentiation, metabolism, and other functions. A study has found that most proteins involved in signal transduction are products of oncogenes or tumor suppressor genes, and their abnormal expression is closely related to the occurrence and development of tumors ( 10 ). The AKT pathway is an important signaling pathway that regulates cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

The effects of the tumor suppressor gene PTEN on the proliferation and apoptosis of breast cancer cells via AKT phosphorylation

Zhang¹,

Ye²,

Lin³

et al. 2023

Transl Cancer Res

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Background The proliferation and apoptosis of cancer cells play important roles in breast carcinomas. However, to date, there have been few reports on the correlation between the expression of PTEN and AKT phosphorylation in breast cancer. This present study investigated the effects of the phosphatase and tensin homology deleted from chromosome 10 ( PTEN ) gene on the proliferation and apoptosis of breast cancer cells through protein kinase B (AKT) phosphorylation. Methods Human breast cancer MDA-MB-231 cells were transfected with the pcDNA3.0 control vector or the pcDNA3.0- PTEN vector for 48 hours. The Cell Counting Kit 8 (CCK-8) was used to detect cell survival rates, double staining was performed to detect apoptosis, and Western blot (WB) analysis was conducted to detect protein expression. The effects of PTEN expression on the cell cycle and apoptosis of human breast cancer cell line MDA-MB-231, and on the levels of phosphorylated AKT protein were further analyzed. Moreover, the relationship between the PTEN gene and clinical features were also analyzed. Results The cell survival rate of cells transfected with pcDNA3.0- PTEN was significantly lower than that of cells transfected with the control pcDNA3.0 vector (55.65%±12.18% vs. 97.32%±12.45%, P=0.004). Compared with the pcDNA3.0 group, the apoptosis rate of the pcDNA3.0- PTEN group was significantly increased (20.65±2.18 vs. 2.32±0.45, P=0.001). The expression of PTEN protein in pcDNA3.0- PTEN group was higher than that in the pcDNA3.0 group, and the expression of the AKT and mTOR proteins was significantly lower than that in pcDNA3.0 group (P<0.05). The expression of PTEN in the lymph node metastasis positive group was significantly higher than that in the lymph node metastasis negative group (P<0.05). The expression of the AKT protein in breast cancer was higher than that in normal breast tissue, and the difference was statistically significant (P<0.01). Conclusions Overexpression of the PTEN gene can promote AKT phosphorylation, increase the apoptotic index of breast cancer cells, and reduce the proliferative activity of breast cancer cells. This provided a new direction for the next treatment of breast cancer, but further clinical research is needed.

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State of the Science on Brain Insulin Resistance and Cognitive Decline Due to Alzheimer’s Disease

Rhea,

Leclerc,

Yassine

et al. 2023

Aging and disease

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Type 2 diabetes mellitus (T2DM) is common and increasing in prevalence worldwide, with devastating public health consequences. While peripheral insulin resistance is a key feature of most forms of T2DM and has been investigated for over a century, research on brain insulin resistance (BIR) has more recently been developed, including in the context of T2DM and non-diabetes states. Recent data support the presence of BIR in the aging brain, even in non-diabetes states, and found that BIR may be a feature in Alzheimer’s disease (AD) and contributes to cognitive impairment. Further, therapies used to treat T2DM are now being investigated in the context of AD treatment and prevention, including insulin. In this review, we offer a definition of BIR, and present evidence for BIR in AD; we discuss the expression, function, and activation of the insulin receptor (INSR) in the brain; how BIR could develop; tools to study BIR; how BIR correlates with current AD hallmarks; and regional/cellular involvement of BIR. We close with a discussion on resilience to both BIR and AD, how current tools can be improved to better understand BIR, and future avenues for research. Overall, this review and position paper highlights BIR as a plausible therapeutic target for the prevention of cognitive decline and dementia due to AD.

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Central administration of insulin and refeeding lead to Akt and ERK phosphorylation in the chicken medulla

Cited by 3 publications

References 30 publications

Recent Research on Mechanisms of Feeding Regulation in Chicks

Recent Research on Mechanisms of Feeding Regulation in Chicks

The effects of the tumor suppressor gene PTEN on the proliferation and apoptosis of breast cancer cells via AKT phosphorylation

State of the Science on Brain Insulin Resistance and Cognitive Decline Due to Alzheimer’s Disease

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