Central Venous Catheter-Related Bloodstream Infection by <i>Corynebacterium striatum</i> Identified by 16S rRNA and <i>rpoB</i> Gene Sequencing

Yang, Haeryong; Kim, Young Jin; Cho, Sun Young; Shin, Eunju; Lee, Hee Joo

doi:10.3343/alm.2015.35.5.548

Cited by 18 publications

(15 citation statements)

References 8 publications

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“…Souza C et al [14] reported that C. striatum clinical strains can tightly adhere to several kinds of environmental surfaces or implanted medical devices [27][28][29] and lead to several kinds of invasive infections. Consistently, most of the C. striatum strains tested in this study showed moderate to strong biofilm abilities and differed greatly.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Pathogenicity of Corynebacterium Striatum Clinical Isolates Collected from Three Tertiary Hospitals in China

Wang¹,

Shi

Zhang³

et al. 2019

Preprint

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Background Antimicrobial resistance and patient-to-patient transmission of Corynebacterium striatum ( C. striatum ) clinical strains were frequently reported in recent years. Even worse, daptomycin resistant isolates were found in some studies and possible resistance mechanism was explored. Also, few investigations revealed the diversity of resistance feature and potential pathogenicity of C. striatum strains with different genotypes. However, less is known about the possible differences of resistance feature and pathogenicity of C. straitum clinical strains from different hospitals at a long distance.Methods C. striatum clinical strains were isolated and identified with VITEK-2 ANC card, MALDI-TOF microTyper and 16S rRNA sequencing technique. Broth microdilution method was used to detect the antibiotic susceptibility profiles of 420 C. striatum clinical isolates, and PFGE method was used to discriminate different clones. Furthermore, in vitro adherence assay and mouse toxicity assay were performed to assess the pathogenicity of the strains with different genotypes.Results 420 C. striatum isolates were all sensitive to vancomycin, linezolid and daptomycin. Based on antibiotic resistance results, 420 strains were classified into 19 resistance patterns, when R1, R2 and R3 patterns accounted for 45.2%(190/420), 20.2%(85/420) and 22.4%(94/420), which were all multi-drug resistant patterns. PFGE typing results showed that 107 C. striatum strains were classified into 52 types (T01-T52), when 4 epidemic clones(T36, T28, T32, T14) accounted for 14.02% (15/107), 11.21%(12/107), 5.61%(6/107) and 3.73%(4/107), respectively. All of these 4 clones belonged to resistance patterns R1, R2 and R3. Among 27 C. striatum strains, 92.6%(25/27) strains showed moderate to strong in vitro adherence abilities, while only 7.4%(2/27) strains showed weak adherence ability on polystyrene surfaces. Furthermore, mouse lethality of different strains differed greatly, when non-dominant clone(Strain NMGYC339, T24) showed the strongest mouse lethality(90.0%).Conclusions The majority of C. striatum strains were multi-drug resistant and few dominant clones could persist for a long time in hospital environment. The in vitro adherence abilities and mouse lethality among different clones differed greatly. The resistance and pathogenicity of C. striatum clinical strains should be paid more attention to, especially for some specific clones at different hospitals.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Pathogenicity of Corynebacterium Striatum Clinical Isolates Collected from Three Tertiary Hospitals in China

Wang¹,

Shi

Zhang³

et al. 2019

Preprint

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“…Though API Coryne system is a useful tool for identifying Corynebacterium species in the clinical laboratory, this may not incorporate all of the tests necessary for the identification of every Corynebacterium species. In recent years, the introduction of molecular methods, especially 16S rRNA gene and rpoB gene sequencing, has improved the ability to identification of Corynebacterium species [ 5 6 10 ]. In our case, the causative organism was misidentified as K. kristinae by the commercial identification kit but was confirmed as C. striatum by 16S rRNA sequence analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Corynebacterium striatum is a commonly-isolated corynebacteria in the clinical microbiology laboratory, although confirmed infections from sterile sites are relatively rare [ 3 ]. C. striatum can cause pulmonary abscess, meningitis, septic arthritis, vertebral osteomyelitis, catheter-related blood stream infection and native- and prosthetic-valve endocarditis [ 2 3 4 5 ]. The aid of molecular diagnostic methods, especially the 16S rRNA and rpoB gene sequencing, has greatly improved the ability to detect C. striatum in clinical specimen [ 5 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…C. striatum can cause pulmonary abscess, meningitis, septic arthritis, vertebral osteomyelitis, catheter-related blood stream infection and native- and prosthetic-valve endocarditis [ 2 3 4 5 ]. The aid of molecular diagnostic methods, especially the 16S rRNA and rpoB gene sequencing, has greatly improved the ability to detect C. striatum in clinical specimen [ 5 6 ]. Here, we report a case of native-valve endocarditis caused by C. striatum, which was accurately identified by 16S ribosomal RNA sequencing in a non-immunocompromised patient.…”

Section: Introductionmentioning

confidence: 99%

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Native Valve Endocarditis due toCorynebacterium striatumconfirmed by 16S Ribosomal RNA Sequencing: A Case Report and Literature Review

2016

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Corynebacterium species are non-fermentous Gram-positive bacilli that are normal flora of human skin and mucous membranes and are commonly isolated in clinical specimens. Non-diphtheriae Corynebacterium are regarded as contaminants when found in blood culture. Currently, Corynebacterium striatum is considered one of the emerging nosocomial agents implicated in endocarditis and serious infections. We report a case of native-valve infective endocarditis caused by C. striatum, which was misidentified by automated identification system but identified accurately by 16S ribosomal RNA sequencing, in a 55-year-old male patient. The patient had two mobile vegetations on his mitral valve, both of which had high embolic risk. Through surgical valve replacement and an antibiotic regimen, the patient recovered completely. In unusual clinical scenarios, C. striatum should not be simply dismissed as a contaminant when isolated from clinical specimens. The possibility of C. striatum infection should be considered even in an immunocompetent patient, and we suggest a genotypic assay, such as 16S rRNA sequencing, to confirm species identity.

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“…www.sochinf.cl Otra herramienta es la secuenciación genética; sin embargo, su disponibilidad es mucho menor y de alto costo 6 . Lamentablemente en este caso no se pudo realizar la secuenciación de la cepa; sin embargo, se aisló el mismo agente en dos muestras tomadas con técnica aséptica en procedimientos quirúrgicos diferentes y se confirmó en las dos oportunidades la identificación de C. striatum por la técnica MALDI-TOF MS.…”

Section: Discussionunclassified

Osteomielitis crónica por Corynebacterium striatum en una adolescente

Beltrán-Arroyave

Díaz-Díaz

Loaiza-Díaz³

2016

Rev. chil. infectol.

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Central Venous Catheter-Related Bloodstream Infection by Corynebacterium striatum Identified by 16S rRNA and rpoB Gene Sequencing

Cited by 18 publications

References 8 publications

Antimicrobial Resistance and Pathogenicity of Corynebacterium Striatum Clinical Isolates Collected from Three Tertiary Hospitals in China

Antimicrobial Resistance and Pathogenicity of Corynebacterium Striatum Clinical Isolates Collected from Three Tertiary Hospitals in China

Native Valve Endocarditis due toCorynebacterium striatumconfirmed by 16S Ribosomal RNA Sequencing: A Case Report and Literature Review

Osteomielitis crónica por Corynebacterium striatum en una adolescente

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