Centrifugal Microfluidic Platform for Ultrasensitive Detection of Botulinum Toxin

Koh, Chung-Yan; Schaff, Ulrich Y.; Piccini, Matthew; Stanker, Larry H.; Cheng, Luisa W.; Ravichandran, Easwaran; Singh, Bal-Ram; Sommer, Gregory J; Singh, Anup K.

doi:10.1021/ac504054u

Cited by 65 publications

(61 citation statements)

References 50 publications

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“…Towards this goal, we have developed multiplexed immunoassays that can be implemented in a portable microfluidic platform developed by our group previously. 29,30 The sensing method used was sandwich immunoassays performed using silica beads (Figure 1). High specificity antibodies (capture antibodies) were covalently attached to the silica beads using a PEG linker.…”

Section: Resultsmentioning

confidence: 99%

Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water

et al. 2016

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Waterborne pathogens pose significant threat to the global population and early detection plays an important role both in making drinking water safe, as well as in diagnostics and treatment of water-borne diseases. We present an innovative centrifugal sedimentation immunoassay platform for detection of bacterial pathogens in water. Our approach is based on binding of pathogens to antibody-functionalized capture particles followed by sedimentation of the particles through a densitymedia in a microfluidic disk. Beads at the distal end of the disk are imaged to quantify the fluorescence and determine the bacterial concentration. Our platform is fast (20 min), can detect as few as $10 bacteria with minimal sample preparation, and can detect multiple pathogens simultaneously. The platform was used to detect a panel of enteric bacteria (Escherichia coli, Salmonella typhimurium, Shigella, Listeria, and Campylobacter) spiked in tap and ground water samples. V C 2016 AIP Publishing LLC. [http://dx

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Section: Resultsmentioning

confidence: 99%

Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water

et al. 2016

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“…To tackle this problem, the capture bead can separate biomarkers from blood sample and enrich these biomarkers to ensure higher signal and lower LOD through sedimentation from density media (Figure 5b). [49] First, the sample containing antigen is mixed with capture beads. Then, fluorescent antibody add in the mixture as the signal tag.…”

Section: Thermoplasticsmentioning

confidence: 99%

Section: Materials For Enhanced Immunoassaymentioning

confidence: 99%

Materials for Microfluidic Immunoassays: A Review

Mou

Jiang

2017

Adv Healthcare Materials

120

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Conventional immunoassays suffer from at least one of these following limitations: long processing time, high costs, poor user‐friendliness, technical complexity, poor sensitivity and specificity. Microfluidics, a technology characterized by the engineered manipulation of fluids in channels with characteristic lengthscale of tens of micrometers, has shown considerable promise for improving immunoassays that could overcome these limitations in medical diagnostics and biology research. The combination of microfluidics and immunoassay can detect biomarkers with faster assay time, reduced volumes of reagents, lower power requirements, and higher levels of integration and automation compared to traditional approaches. This review focuses on the materials‐related aspects of the recent advances in microfluidics‐based immunoassays for point‐of‐care (POC) diagnostics of biomarkers. We compare the materials for microfluidic chips fabrication in five aspects: fabrication, integration, function, modification and cost, and describe their advantages and drawbacks. In addition, we review materials for modifying antibodies to improve the performance of the reaction of immunoassay. We also review the state of the art in microfluidic immunoassays POC platforms, from the laboratory to routine clinical practice, and also commercial products in the market. Finally, we discuss the current challenges and future developments in microfluidic immunoassays.

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“…With the capability of efficiently transforming chemical or biological reaction into centrifugal microfluidic chip, optical detection methods are widely used in chemical industry [15], biological process [16], medical diagnostics [17], healthcare [18], environmental monitoring [19], military defense [20], and scientific research. Optical analysis possesses higher sensitivity and immunity to electromagnetic interference compared with other analytical methods.…”

Section: Introductionmentioning

confidence: 99%

Centrifugal Microfluidic Integrated Optical System for Sensing Pesticide Residues

Liu¹,

Song²,

Wang³

et al. 2017

Biosens J

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We proposed and demonstrated an effective and non-destructive centrifugal microfluidic sensor (CMS) for analysis of organophosphorus pesticides (OPPs). The CMS was designed to have a sequential sample injection with two capillary valves. The actuation mechanism of CMS was controlled by centrifugal force, i.e. relative centrifugal force (×g). The OPPs analyte in CMS could be successfully derived into a mixer at 34 g and then spined into a detection reservoir at 537 g. The experiment results indicated that limit of detection for OPPs using CMS was 0.05 ppm, a linear relationship between OPPs logarithmic concentration (ppm) and inhibition rate (%) was 0.9215. Furthermore, series fresh vegetables were extracted and assayed for OPPs residue detection by using CMS. It was effective for OPPs detection. A non-destructive, rapid, reliable, and sensitive approach to OPPs analysis was demonstrated. This in turn opens up opportunities for healthcare applications, notably environmental monitoring, foods safety and other fields.

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Centrifugal Microfluidic Platform for Ultrasensitive Detection of Botulinum Toxin

Cited by 65 publications

References 50 publications

Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water

Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water

Materials for Microfluidic Immunoassays: A Review

Centrifugal Microfluidic Integrated Optical System for Sensing Pesticide Residues

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