Centrifugal partition chromatography elution gradient for isolation of sesquiterpene lactones and flavonoids from Anvillea radiata

Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice

“…& Dur (Asteraceae), which is mainly distributed in northern Africa, is used by populations and traditional healers in infusions or decoctions of the leaves and flowers to treat a variety of ailments such as chest cold, gastrointestinal, liver and pulmonary disorders, and postprandial hyperglycemia (Hammiche and Maiza, 2006;Ghourri et al, 2013;Fakchich and Elachouri, 2014). Previous phytochemical reports identified two main families in A. radiata aerial parts, i.e., germacranolides such as 9α-hydroxyparthenolide and 8α, 9αepoxyparthenolide isolated in chloroform extracts, and a less abundant fraction of phenolics (e.g., derivatives of dicaffeoylquinic acid), including flavonoids (both glycosides and aglycones) in aqueous/alcoholic extracts (Dendougui et al, 2006;Moumou et al, 2014;Destandau et al, 2015;Boukhris et al, 2016). Aside from these active compounds, saponins, tannins, fatty acids, and various types of secondary metabolites, including sterols, terpenoids, etc.…”

Purification of vandaterosides from Vanda teres (Orchidaceae) by stepwise gradient centrifugal partition chromatography

“…To increase the polarity of the solvent system, we decided to add 1‐butanol, and chose water saturated in 1‐butanol as the suitable stationary (lower) phase. Instead of performing linear gradient CPC, as reported in various papers , we decided to try a stepwise gradient in one run, which consists of increasing the polarity of the mobile phase by combining three plateau reached by linear gradients. A three‐stage gradient was considered accordingly to the ternary diagram, by selecting the most extreme biphasic systems: ethyl acetate/water (saturated in 1‐butanol) (50:50) and water/1‐butanol (50:50), as well as an intermediate system with medium polarity, ethyl acetate/1‐butanol/water (30:20:50) (Fig.…”

Centrifugal partition chromatography - A review of recent applications and some classic references

“…Examples of separations with the use of gradient elution include many applications. Just a few of them are the separation of amino acids and peptides with hexane/1‐butanol/water two‐phase systems , anthocyanins with ethyl acetate/1‐butanol/water biphasic solvent system , quinolines with heptane/water/acetonitrile and heptane/acetonitrile/methanol ternary two‐phase solvent systems , or sesquiterpene lactones with heptane/ethyl acetate/methanol/water two‐phase solvent system .…”