Centrifugo-thermopneumatic fluid control for valving and aliquoting applied to multiplex real-time PCR on off-the-shelf centrifugal thermocycler

Keller, M.; Wadle, Simon; Paust, Nils; Dreesen, Lucas; Nuese, C.; Strohmeier, Oliver; Zengerle, Roland; Stetten, Felix von

doi:10.1039/c5ra16095b

Cited by 35 publications

(29 citation statements)

References 22 publications

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“…2D). 67,68 Airdried reagents for the PCR reaction are dissolved by the sample inside of the chambers. Thermocycling is conducted in a commercial thermocycler.…”

Section: Centrifugal Microfluidic Devicesmentioning

confidence: 99%

Polymerase chain reaction in microfluidic devices

2016

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The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude. Since the first application of PCR in a microfluidic device was developed in 1998, an increasing number of researchers have continued the development of microfluidic PCR systems. In this review, we introduce recent developments in microfluidic-based space and time domain devices as well as discuss various designs integrated with multiple functions for sample preparation and detection. The development of isothermal nucleic acid amplification and digital PCR microfluidic devices within the last five years is also highlighted. Furthermore, we introduce various commercial microfluidic PCR devices.

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“…2D). 67,68 Airdried reagents for the PCR reaction are dissolved by the sample inside of the chambers. Thermocycling is conducted in a commercial thermocycler.…”

Section: Centrifugal Microfluidic Devicesmentioning

confidence: 99%

Polymerase chain reaction in microfluidic devices

2016

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“…The further development of centrifugal microfluidics, particularly for bacteria detection, is integrating and automating the different analysis steps into a single device. This group of devices is known as “Lab Disk” [82]. A Lab Disk includes cell lysis, DNA extraction, amplification, and detection, in an integrated and automated format, to provide “sample in, answer out” for the identification of bacteria.…”

Section: Transient Pcrmentioning

confidence: 99%

“…With a single PCR chamber, this device was not suitable for multiplexing. Keller et al reported multiplexed real-time PCR of E. coli employing a centrifuge-thermopneumatic fluid control system that uses the temperature-induced partial vapor pressure change of an enclosed gas volume for valving and aliquoting [82].…”

Section: Transient Pcrmentioning

confidence: 99%

Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology

2019

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Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers. The paper also discusses the recent development of isothermal nucleic acid amplification and droplet-based microfluidics devices. We discuss recent microfluidic techniques for sample preparation prior to the amplification process.

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“…When the temperature increased, gas expanded and drove the liquid to pass the crest point of the siphon. In this paper, CTP two-stage aliquoting was also demonstrated [ 45 ].…”

Section: Advanced Unit Operationsmentioning

confidence: 99%

A Review of Biomedical Centrifugal Microfluidic Platforms

et al. 2016

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Centrifugal microfluidic or lab-on-a-disc platforms have many advantages over other microfluidic systems. These advantages include a minimal amount of instrumentation, the efficient removal of any disturbing bubbles or residual volumes, and inherently available density-based sample transportation and separation. Centrifugal microfluidic devices applied to biomedical analysis and point-of-care diagnostics have been extensively promoted recently. This paper presents an up-to-date overview of these devices. The development of biomedical centrifugal microfluidic platforms essentially covers two categories: (i) unit operations that perform specific functionalities, and (ii) systems that aim to address certain biomedical applications. With the aim to provide a comprehensive representation of current development in this field, this review summarizes progress in both categories. The advanced unit operations implemented for biological processing include mixing, valving, switching, metering and sequential loading. Depending on the type of sample to be used in the system, biomedical applications are classified into four groups: nucleic acid analysis, blood analysis, immunoassays, and other biomedical applications. Our overview of advanced unit operations also includes the basic concepts and mechanisms involved in centrifugal microfluidics, while on the other hand an outline on reported applications clarifies how an assembly of unit operations enables efficient implementation of various types of complex assays. Lastly, challenges and potential for future development of biomedical centrifugal microfluidic devices are discussed.

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Centrifugo-thermopneumatic fluid control for valving and aliquoting applied to multiplex real-time PCR on off-the-shelf centrifugal thermocycler

Abstract: Microfluidic App for automation of geometrically multiplexed real-time PCR on off-the-shelf PCR thermocycler.

Cited by 35 publications

References 22 publications

Polymerase chain reaction in microfluidic devices

Polymerase chain reaction in microfluidic devices

Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology

A Review of Biomedical Centrifugal Microfluidic Platforms

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