Centriole ultrastructure and its possible role in microtubule formation in an aquatic fungus

McNitt, Rand E.

doi:10.1007/bf01666353

Cited by 19 publications

(13 citation statements)

References 37 publications

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“…Furthermore, interestingly, the 9-fold symmetry of the CID raises the possibility that it might contribute to establishing the likewise symmetry of the entire centriole. Intriguingly, a structure with 9-fold rotational symmetry termed the stellate granule is also present in the lumen of the cartwheel of the fungus Phlyctochytrium irregulare [26], suggesting that an inner structure interacting with SAS-6 rings could be a more general theme.…”

Section: A B Cmentioning

confidence: 99%

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

et al. 2013

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Section: A B Cmentioning

confidence: 99%

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

et al. 2013

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“…3], in the parabasalid protist Pseudotrichonympha, [(Gibbons and Grimstone, 1960) plate 351, Fig. 7] and in the chytridiomycete fungus Phlyctochytrium irregulare Koch [(McNitt, 1974) Fig. 3].…”

Section: Discussionmentioning

confidence: 99%

The ultrastructure of theChlamydomonas reinhardtiibasal apparatus: identification of an early marker of radial asymmetry inherent in the basal body

Geimer

Melkonian

2004

Journal of Cell Science

135

193

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The biflagellate unicellular green alga Chlamydomonas reinhardtii is a classic model organism for the analysis of flagella and their organizers, the basal bodies. In this cell, the two flagella-bearing basal bodies, along with two probasal bodies and an array of fibers and microtubules, form a complex organelle called the basal apparatus. The ultrastructure of the basal apparatus was analysed in detail by serial thin-section electron microscopy of isolated cytoskeletons and several newly discovered features are described, including a marker for the rotational asymmetry inherent in the basal bodies and probasal bodies. In addition, the complex three-dimensional basal apparatus ultrastructure is resolved and illustrated, including the attachment sites of all basal apparatus elements to specific microtubular triplets of the basal bodies and probasal bodies. These data will facilitate both the localization of novel basal apparatus proteins and the analysis of mutants and RNA interference cells with only subtle defects in basal apparatus ultrastructure. The early harbinger of radial asymmetry described here could play a crucial role during basal body maturation by orienting the asymmetric attachment of the various associated fibers and therefore might define the orientation of the basal bodies and, ultimately, the direction of flagellar beating.

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“…2D and 2I), although it is discontinuous and smaller in diameter than the fCID. Moreover, a density that may be related to either CID or fCID is apparent inside the hub in other species, including Chlamydomonas, Chytrid and several insects (Geimer & Melkonian, 2004;Guichard et al, 2017;O'Toole & Dutcher, 2014;Olson & Fuller, 1968;McNitt 1974;Gottardo et al, 2015;Uzbekov et al, 2018, Klena et al) (Fig. 6A).…”

Section: Conserved Features In Cartwheel Architecturementioning

confidence: 93%

Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Nazarov

Bezler

Hatzopoulos

et al. 2020

Preprint

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Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers of SAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellate Trichonympha spp. was obtained previously, but higher resolution analysis is likely to reveal novel features. Using sub-tomogram averaging (STA) in T. spp. and Trichonympha agilis, we delineate the architecture of centriolar microtubules, pinhead and A-C-linker. Moreover, we report ~25 Å resolution maps of the central cartwheel, revealing notably polarized cartwheel inner densities (CID). Furthermore, STA of centrioles from the distant flagellate Teranympha mirabilis uncovers similar cartwheel architecture and a distinct filamentous CID. Fitting the CrSAS-6 crystal structure into the flagellate maps and analyzing cartwheels generated in vitro indicates that SAS-6 rings can directly stack onto one another in two alternating configurations: with a slight rotational offset and in register. Overall, improved STA maps in three flagellates enabled us to unravel novel architectural features, including of centriole polarity and cartwheel stacking, thus setting the stage for an accelerated elucidation of underlying assembly mechanisms.

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Centriole ultrastructure and its possible role in microtubule formation in an aquatic fungus

Cited by 19 publications

References 37 publications

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

The ultrastructure of theChlamydomonas reinhardtiibasal apparatus: identification of an early marker of radial asymmetry inherent in the basal body

Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

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