CeO<sub>2</sub>nanoparticles induce DNA damage towards human dermal fibroblasts<i>in vitro</i>

Auffan, Mélanie; Rose, Jérôme; Orsière, Thierry; Méo, Michel De; Thill, Antoine; Zeyons, Ophélie; Proux, Olivier; Armand, Michel; Chaurand, Perrine; Spalla, Olivier; Botta, A.; Wiesner, Mark R.; Bottero, Jean‐Yves

doi:10.1080/17435390902788086

Cited by 193 publications

(126 citation statements)

References 44 publications

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“…Surface area is one of the primary factors initially hypothesized to be related to novel NP toxicity [2,3]. Auffan et al [49] showed that the toxicity of cerium oxide NPs to human fibroblast cells was higher for NPs compared with bulk particles on a mass basis but similar on a surface-areanormalized basis. Furthermore, surface area was an important predictor for toxicity of silica NPs with algae [50].…”

Section: Steps In Conducting Ecotoxicity Tests With Cnpsmentioning

confidence: 99%

Methodological considerations for testing the ecotoxicity of carbon nanotubes and fullerenes: Review

Petersen

Henry

2011

Enviro Toxic and Chemistry

114

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Abstract-The recent emergence of manufactured nanoparticles (NPs) that are released into the environment and lead to exposure in organisms has accelerated the need to determine NP toxicity. Techniques for measuring the toxicity of NPs (nanotoxicology) in ecological receptors (nanoecotoxicology) are in their infancy, however, and establishing standardized ecotoxicity tests for NPs are presently limited by several factors. These factors include the extent of NP characterization necessary (or possible) before, during, and after toxicity tests such that toxic effects can be related to physicochemical characteristics of NPs; determining uptake and distribution of NPs within exposed organisms (does uptake occur or are effects exerted at organism surfaces?); and determining the appropriate types of controls to incorporate into ecotoxicity tests with NPs. In this review, the authors focus on the important elements of measuring the ecotoxicity of carbon NPs (CNPs) and make recommendations for ecotoxicology testing that should enable more rigorous interpretations of collected data and interlaboratory comparisons. This review is intended to serve as a next step toward developing standardized tests that can be incorporated into a regulatory framework for CNPs. Environ. Toxicol. Chem.

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Section: Steps In Conducting Ecotoxicity Tests With Cnpsmentioning

confidence: 99%

Methodological considerations for testing the ecotoxicity of carbon nanotubes and fullerenes: Review

Petersen

Henry

2011

Enviro Toxic and Chemistry

114

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“…In several reports, CeO 2 NPs have been shown to be nontoxic to murine insulinoma βTC-tet cells, 29 human umbilical vein endothelial cells and human lung fibroblasts, 30 RAW 264.7 macrophage cells, 27 human breast epithelial cells, 31 murine macrophages, 32 and human colon cells. 33 In contrast, several studies have demonstrated a cytotoxic effect of CeO 2 NPs in normal cells, including human dermal fibroblasts 5 and human peripheral blood monocytes, 34 which was attributed to oxidative stress in the former and mitochondrial damage along with overexpression of apoptosis-inducing factor in the latter. In our study, Au/CeO 2 NPs showed minimal toxicity (~15%-17%) towards normal RAW 264.7 macrophage cells (Figure 4), and thus CeO 2 NPs and Au/CeO 2 NPs can be deemed nontoxic because materials with cell viability 80% are usually considered cytocompatible.…”

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confidence: 94%

“…As mentioned before, although CeO 2 NPs possess unique autoregenerative antioxidant activity, they can also behave as pro-oxidants depending on the compartment where they become localized inside the cell. 5,27,34 Obviously, the tumor progression, invasion, metastasis, and the acidic tumor environment are closely interrelated. 42 The difference between normal cells and cancer cells may also play a crucial role in determining the antioxidant or pro-oxidant behavior of CeO 2 NPs.…”

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confidence: 99%

Cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles

Babu¹,

Anandkumar²,

Tsai³

et al. 2014

IJN

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Background Cerium oxide nanoparticles (CeO 2 ) have been shown to be a novel therapeutic in many biomedical applications. Gold (Au) nanoparticles have also attracted widespread interest due to their chemical stability and unique optical properties. Thus, decorating Au on CeO 2 nanoparticles would have potential for exploitation in the biomedical field. Methods In the present work, CeO 2 nanoparticles synthesized by a chemical combustion method were supported with 3.5% Au (Au/CeO 2 ) by a deposition-precipitation method. The as-synthesized Au, CeO 2 , and Au/CeO 2 nanoparticles were evaluated for antibacterial activity and cytotoxicity in RAW 264.7 normal cells and A549 lung cancer cells. Results The as-synthesized nanoparticles were characterized by X-ray diffraction, scanning and transmission electron microscopy, and ultraviolet-visible measurements. The X-ray diffraction study confirmed the formation of cubic fluorite-structured CeO 2 nanoparticles with a size of 10 nm. All synthesized nanoparticles were nontoxic towards RAW 264.7 cells at doses of 0–1,000 μM except for Au at >100 μM. For A549 cancer cells, Au/CeO 2 had the highest inhibitory effect, followed by both Au and CeO 2 which showed a similar effect at 500 and 1,000 μM. Initial binding of nanoparticles occurred through localized positively charged sites in A549 cells as shown by a shift in zeta potential from positive to negative after 24 hours of incubation. A dose-dependent elevation in reactive oxygen species indicated that the pro-oxidant activity of the nanoparticles was responsible for their cytotoxicity towards A549 cells. In addition, cellular uptake seen on transmission electron microscopic images indicated predominant localization of nanoparticles in the cytoplasmic matrix and mitochondrial damage due to oxidative stress. With regard to antibacterial activity, both types of nanoparticles had the strongest inhibitory effect on Bacillus subtilis in monoculture systems, followed by Salmonella enteritidis , Escherichia coli , and Staphylococcus aureus , while, in coculture tests with Lactobacillus plantarum , S. aureus was inhibited to a greater extent than the other bacteria. Conclusion Gold-supported CeO 2 nanoparticles may be a potential nanomaterial for in vivo application owing to their biocompatible and antibacterial properties.

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“…However, the reduction of the Ce(IV) into Ce(III) at the surface of the nanoceria correlates with the toxicity. Using XANES at Ce L3-edge, Thill et al 49,95 and Auffan et al 98 showed that the cytotoxicity/genotoxicity of nanoceria could be related to the reduction of surface Ce(IV) atoms to Ce(III). But, further research is needed to find out whether the oxidative activity of ceria could be responsible.…”

mentioning

confidence: 99%

Environmental release, fate and ecotoxicological effects of manufactured ceria nanomaterials

Collin

Auffan

Johnson

et al. 2014

Environ. Sci.: Nano

107

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uses. This has led to a number of publications on the toxicological effects of nanoceria in ecological receptor species, but only limited information is available on possible environmental releases, concentrations in environmental media, or environmental transformations. Increasing material flows of nanoceria in many applications is likely to result in increasing releases to air, water and soils however; insufficient information was available to estimate aquatic exposures that would result in acute or chronic toxicity. The purpose of this review is to identify which areas are lacking in data to perform either regional or site specific ecological risk assessments. While estimates can be made for releases from use as a diesel fuel additive, and predicted toxicity is low in most terrestrial species tested to date, estimates for releases from other uses are difficult at this stage. We

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CeO₂nanoparticles induce DNA damage towards human dermal fibroblastsin vitro

Cited by 193 publications

References 44 publications

Methodological considerations for testing the ecotoxicity of carbon nanotubes and fullerenes: Review

Methodological considerations for testing the ecotoxicity of carbon nanotubes and fullerenes: Review

Cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles

Environmental release, fate and ecotoxicological effects of manufactured ceria nanomaterials

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CeO2nanoparticles induce DNA damage towards human dermal fibroblastsin vitro

Cited by 193 publications

References 44 publications

Methodological considerations for testing the ecotoxicity of carbon nanotubes and fullerenes: Review

Methodological considerations for testing the ecotoxicity of carbon nanotubes and fullerenes: Review

Cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles

Environmental release, fate and ecotoxicological effects of manufactured ceria nanomaterials

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Product

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CeO₂nanoparticles induce DNA damage towards human dermal fibroblastsin vitro