2006
DOI: 10.1016/j.fct.2006.06.018
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Cephalopod tropomyosins: Identification as major allergens and molecular cloning

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Cited by 66 publications
(41 citation statements)
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“…As observed in our study, this 42 kDa major allergen and several additional minor allergens were detected only in the raw extract. Moreover, patient 13 had no detectable specific IgE to the 36 kDa which had only IgE-binding proteins to the raw extract. These findings show that all those allergens were heat-sensitive proteins and boiling has decreased the allergenicity of the allergen extract.…”
Section: Discussionmentioning
confidence: 99%
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“…As observed in our study, this 42 kDa major allergen and several additional minor allergens were detected only in the raw extract. Moreover, patient 13 had no detectable specific IgE to the 36 kDa which had only IgE-binding proteins to the raw extract. These findings show that all those allergens were heat-sensitive proteins and boiling has decreased the allergenicity of the allergen extract.…”
Section: Discussionmentioning
confidence: 99%
“…Then 50 毺g of prawn extract was applied to immobilized non-linear pH [3][4][5][6][7][8][9][10] gradient strip of 7 cm length for rehydration overnight (12)(13)(14) h). Isoelectric focusing was run at 20 曟 using the Protean IEF Cell Apparatus (BioRad, USA) with the following voltage/time gradient: 100 V for 1 min, 250 V for 30 min, 4 000 V for 2 h and 4 000 V for 10 000 V-h. Before transferring the IPG strip onto the second dimension, the strip was equilibrated sequentially for 10 min in a buffer containing 65 mM dithio-threitol and then 135 mM iodoacetamide in 125 mM Tris-HCl, pH 6.8, 6 M urea, 2% SDS, 30% glycerol and 0.01% bromophenol blue.…”
Section: Two-dimensional (2-de) Gel Electrophoresismentioning
confidence: 99%
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“…The allergen in shrimp is tropomyosin, a heat-stable coiled-coil protein with a molecular weight of between 34 and 38 kDa (Daul et al, 1994;Shanti et al, 1993;Jeoung et al, 1997;Leung et al,1996;Motoyama et al, 2006). It was previously found that in an aqueous extract of shrimp protein, tropomyosin was adsorbed at higher levels onto stainless steel surfaces than other proteins in the extract (Thammathongchat et al, 2009).…”
Section: Introductionmentioning
confidence: 99%
“…The main allergens in crustaceans and cephalopods are tropomyosin, a 34-38 kDa microfibrillar protein with amino acid sequence highly conserved among shellfish [116,117], and arginine kinase [118].…”
Section: Allergen Detectionmentioning
confidence: 99%