Ceramic-based multisite microelectrode arrays for simultaneous measures of choline and acetylcholine in CNS

Burmeister, Jason J.; Pomerleau, François; Huettl, Peter; Gash, Clelland R.; Werner, Catherine E.; Bruno, John P.; Gerhardt, Greg A.

doi:10.1016/j.bios.2007.12.013

Cited by 123 publications

(124 citation statements)

References 45 publications

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“…This was 1-3 orders of magnitude higher than what was observed for artificial receptorbased systems [2,4]. It was also comparable to the acetylcholine detection limit achieved by acetylcholine enzymebased sensors [6,8]. Acetylcholine in the royal jelly was detected to be 912758 mg/g, which was in agreement with the known acetylcholine concentration (958 mg/g) in the royal jelly.…”

supporting

confidence: 83%

A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction

Wei¹,

Wei²,

Kang³

et al. 2009

Electrophoresis

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A novel method for highly sensitive detection of acetylcholine in royal jelly was proposed by using CE coupled with electrogenerated chemiluminescence (ECL). Acetylcholine, which could not react with Tris(2,2'-bipyridine)ruthenium(II) to strengthen its ECL signals, decomposed into trimethylamine and strengthened the ECL signals sharply when it was heated to its melting point. This reaction needed no additional reagent and it was mild, simple, stable and rapid, without any side reaction. By combining the above process with CE separation technique, trimethylamine in royal jelly was completely separated from interfering substances and was successfully detected within 4 min. The limit of detection for acetylcholine was found to be 6.3 x 10(-8) g/mL with a signal-to-noise ratio of 3:1. Acetylcholine in the royal jelly was detected to be 912+/-58 microg/g. The recoveries of acetylcholine chloride in the sample were in the ranges of 92-106%. The coefficients of variation for intra-day and inter-day reproducibility were equal to or less than 4.9 and 6.8%, respectively.

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supporting

confidence: 83%

A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction

Wei¹,

Wei²,

Kang³

et al. 2009

Electrophoresis

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“…However, cognitive processes such as attention and arousal, and physiological processes like network rhythms, dynamically change in less than a second. Recent improvements of amperometric recording techniques allow fast, subsecond measurement of extracellular ACh concentration in the brain in vivo (Parikh et al, 2007;Burmeister et al, 2008). With these new tools, old theories regarding the cholinergic system and brain function can now be tested using more appropriate physiological time scales (Ͻ1 s).…”

Section: Review Of Zhang Et Almentioning

confidence: 99%

“…5F). Direct measurements of ACh concurrent with choline (Burmeister et al, 2008) may clarify the nature of observed decoupling between choline concentration and putative medial septal cholinergic neuron firing rate.…”

Section: Review Of Zhang Et Almentioning

confidence: 99%

Decoupling of Acetylcholine Influx and Theta Power in the Hippocampus

Young¹,

Jackson²

2011

J. Neurosci.

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“…Recently, enzyme electrodes have also been integrated on microfabricated probes carrying multiple electrodes [5,6,25,39]. The utilized functionalization methods are similar to previously used single-electrode functionalization methods (drop/dip coating) and are thus limited in upscaling to larger multielectrode ensembles.…”

Section: Introductionmentioning

confidence: 99%

Multisite monitoring of choline using biosensor microprobe arrays in combination with CMOS circuitry

Frey¹,

Rothe²,

Heer³

et al. 2014

Biomedical Engineering / Biomedizinische Technik

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A miniature device enabling parallel in vivo detection of the neurotransmitter choline in multiple brain regions of freely behaving rodents is presented. This is achieved by combining a biosensor microprobe array with a custom-developed CMOS chip. Each silicon microprobe comprises multiple platinum electrodes that are coated with an enzymatic membrane and a permselective layer for selective detection of choline. The biosensors, based on the principle of amperometric detection, exhibit a sensitivity of 157 ± 35 µA mM -1 cm -2 , a limit of detection of below 1 µM, and a response time in the range of 1 s. With on-chip digitalization and multiplexing, parallel recordings can be performed at a high signal-to-noise ratio with minimal space requirements and with substantial reduction of external signal interference. The layout of the integrated circuitry allows for versatile configuration of the current range and can, therefore, also be used for functionalization of the electrodes before use. The result is a compact, highly integrated system, very convenient for on-site measurements.

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Ceramic-based multisite microelectrode arrays for simultaneous measures of choline and acetylcholine in CNS

Cited by 123 publications

References 45 publications

A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction

A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction

Decoupling of Acetylcholine Influx and Theta Power in the Hippocampus

Multisite monitoring of choline using biosensor microprobe arrays in combination with CMOS circuitry

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