Ceramic Microsystem Incorporating a Microreactor with Immobilized Biocatalyst for Enzymatic Spectrophotometric Assays

Baeza, Mireia; López, Carmen; Alonso, J.; López-Santı́n, Josep; Álvaro, Gregorio

doi:10.1021/ac902267f

Cited by 33 publications

(23 citation statements)

References 26 publications

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“…β-Galactosidase Silica streptavidin-coated microspheres Conjugated to biotin via amide coupling of aminocaproyl spacer, and then bound to the microbeds [110]. Glyoxal-agarose on low temperature cofired ceramics Directly immobilized on activated glyoxal-agarose gels [112].…”

Section: Fumarasementioning

confidence: 99%

“…Bio-microreactors with enzymes immobilized on beads have been prepared by filling the reaction chamber with enzyme-immobilized particles. In this case, various materials were used, such as controlled pore glass [96,[107][108][109], microspheres [110], activated agarose [111,112], and different types of magnetic beads [113][114][115]. Both covalent immobilization and physical interactions have been used in these systems with good results.…”

Section: Magnetic Beadsmentioning

confidence: 99%

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Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Laurenti¹,

Vianna²

2016

Biocatalysis

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Microfluidic reaction devices are a very promising technology for chemical and biochemical processes. In microreactors, the micro dimensions, coupled with a high surface area/volume ratio, permit rapid heat exchange and mass transfer, resulting in higher reaction yields and reaction rates than in conventional reactors. Moreover, the lower energy consumption and easier separation of products permit these systems to have a lower environmental impact compared to macroscale, conventional reactors. Due to these benefits, the use of microreactors is increasing in the biocatalysis field, both by using enzymes in solution and their immobilized counterparts. Following an introduction to the most common applications of microreactors in chemical processes, a broad overview will be given of the latest applications in biocatalytic processes performed in microreactors with free or immobilized enzymes. In particular, attention is given to the nature of the materials used as a support for the enzymes and the strategies employed for their immobilization. Mathematical and engineering aspects concerning fluid dynamics in microreactors were also taken into account as fundamental factors for the optimization of these systems.

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Section: Fumarasementioning

confidence: 99%

Section: Magnetic Beadsmentioning

confidence: 99%

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Laurenti¹,

Vianna²

2016

Biocatalysis

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“…Similar technique was used for preparation of enzyme-immobilized microfluidic reactors. Min et al described an enzyme immobilization method that combined the electrostatic interaction and the film overlay techniques [21]. Complex of acetylcholinesterase (AChE) and the negatively-charged alginate polymer was electrostatically adsorbed on the positively charged polyethyleneimine-coated capillary surface, followed by entrapment of AChE through the formation of a chitosan-thin film over the adsorbed enzyme.…”

Section: Fabrication Of Enzyme-immobilized Microreactormentioning

confidence: 99%

Application of Enzyme-Immobilization Technique for Microflow Reactor

Yamaguchi¹,

Honda²,

Miyazaki³

2016

J Flow Chem

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The microreaction technology which is an interdisciplinary science and engineering, have attracted attention in many fields in the past years. Several microreactors have been developed. Enzyme is one of the catalysts, which is useful in substance production in an environmentally friendly way, and has high potential for the preparation of chiral compounds. These features are suitable for pharmaceutical process. However, not so many enzymatic processes were commercialized, because of problems in stability of enzyme molecule, cost, and efficiency of the reactions. Thus, there have been demands for innovation in process engineering particularly for enzymatic reactions, and microreaction devices can be a strong tool for the development of enzyme processes. In this minireview, we summarize fundamental immobilization techniques to develop enzyme microreactor. Some important applications of this technology toward the chemical processing are also included.

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“…52 Enzyme-kinetic study has been performed in microfluidic formats. [53][54][55][56][57][58] Important to proteomics, enzymatic digestion before MALDI-TOF/MS (Matrix-assisted laser desorption-ionization time-of-fly/mass spectrometry) peptide mapping of a protein has been explored in microfluidic devices. [59][60][61][62][63][64][65][66] Compared to conventional in-solution enzyme digestion, which is time consuming and offers limited sensitivity, microfluidic formats have shown high conversion rates, facile replacement of inactivated enzyme, and long-term stability.…”

Section: Introductionmentioning

confidence: 99%

Protein immobilization techniques for microfluidic assays

2013

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Microfluidic systems have shown unequivocal performance improvements over conventional bench-top assays across a range of performance metrics. For example, specific advances have been made in reagent consumption, throughput, integration of multiple assay steps, assay automation, and multiplexing capability. For heterogeneous systems, controlled immobilization of reactants is essential for reliable, sensitive detection of analytes. In most cases, protein immobilization densities are maximized, while native activity and conformation are maintained. Immobilization methods and chemistries vary significantly depending on immobilization surface, protein properties, and specific assay goals. In this review, we present trade-offs considerations for common immobilization surface materials. We overview immobilization methods and chemistries, and discuss studies exemplar of key approaches—here with a specific emphasis on immunoassays and enzymatic reactors. Recent “smart immobilization” methods including the use of light, electrochemical, thermal, and chemical stimuli to attach and detach proteins on demand with precise spatial control are highlighted. Spatially encoded protein immobilization using DNA hybridization for multiplexed assays and reversible protein immobilization surfaces for repeatable assay are introduced as immobilization methods. We also describe multifunctional surface coatings that can perform tasks that were, until recently, relegated to multiple functional coatings. We consider the microfluidics literature from 1997 to present and close with a perspective on future approaches to protein immobilization.

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Ceramic Microsystem Incorporating a Microreactor with Immobilized Biocatalyst for Enzymatic Spectrophotometric Assays

Cited by 33 publications

References 26 publications

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Application of Enzyme-Immobilization Technique for Microflow Reactor

Protein immobilization techniques for microfluidic assays

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