Cerebral vasoconstrictor mediators

Toda, Noboru; Okamura, Tomio

doi:10.1016/0163-7258(93)90061-h

Cited by 5 publications

(3 citation statements)

References 133 publications

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“…The fibres responded to several contractile agonists including endothelin‐1, endothelin‐2, prostaglandin F2α and ACh. It has been demonstrated that endothelin and prostaglandin F2α induce vasoconstriction in cerebral arteries (Toda & Okamura, 1993; Petersson et al 1996). Endothelin‐1 plays roles in the maintenance of cerebral vascular tone as well as in the pathogenesis of cerebral vasospasm (Goto et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Contractile responses of smooth muscle cells differentiated from rat neural stem cells

Oishi

Ogawa

Gamoh

et al. 2002

The Journal of Physiology

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To characterize the functional differentiation of neural stem cells into smooth muscle cells, multipotent stem cells in the central nervous system (CNS) were isolated from rat embryonic day 14 (E14) cortex and cultured by neurosphere formation in serum‐free medium in the presence of 10 ng ml−1 of basic fibroblast growth factor. Differentiation was induced by the addition of 10 % fetal bovine serum to low‐density cultures (2.5 × 103 cells cm−2). Immunological analyses and reverse transcriptase‐polymerase chain reaction indicated that the differentiated cells expressed smooth‐muscle‐specific marker proteins such as SM‐1, SM‐2, and SMemb myosin heavy chains, SM‐22, basic calponin and α‐smooth‐muscle actin, but not the astrocyte marker glial fibrillary acidic protein. To examine whether smooth‐muscle‐like cells that are differentiated from CNS stem cells possess the characteristics of contractile smooth muscle, we prepared reconstituted collagen gel fibres and measured their contractile tension. The reconstituted fibres were prepared by thermal gelation of collagen and the differentiated cells. The fibres contracted in response to treatment with KCl (80 mm), ACh (100 μm), endothelin‐1 (10 nm), endothelin‐2 (10 nm), and prostaglandin F2α (100 μm). ACh‐induced contraction was partially inhibited by the L‐type voltage‐dependent Ca2+ channel inhibitor nifedipine and by the intracellular Ca2+ chelator 1,2‐bis (2‐aminophenoxy) ethane‐N,N,N’,N'‐tetraacetic acid acetoxymethyl ester, the myosin light chain kinase inhibitor ML‐9, the Rho kinase inhibitor Y‐27632, dibutyryl cAMP and 8‐bromo‐cGMP. These results suggest that CNS stem cells give rise to smooth muscle cells in vitro that have an identical contractile function to smooth muscle in vivo.

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Section: Discussionmentioning

confidence: 99%

Contractile responses of smooth muscle cells differentiated from rat neural stem cells

Oishi

Ogawa

Gamoh

et al. 2002

The Journal of Physiology

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“…5-HT, ET-1, prostaglandin F2=, angiotensin II and noradrenaline have been demonstrated to induce vasoconstriction of cerebral arteries (15,16). Isometric force measurements revealed contraction of the fibers in response to these cerebrovasoconstrictors.…”

Section: Discussionmentioning

confidence: 99%

“…However, there was virtually no contractile response to histamine. In contrast, isolated cerebral arteries from various species contract in response to histamine via histamine H1 receptors (16). RT-PCR analysis of total RNA isolated from the smooth muscle fibers, using sequencespecific oligonucleotide primers designed with reference to the published sequence of rat histamine H1 and H2 subtype genes, revealed that mRNA for H 1 subtype was present in smooth muscle fibers.…”

Section: Discussionmentioning

confidence: 99%

Contractile Responses and Myosin Phosphorylation in Reconstituted Fibers of Smooth Muscle Cells From the Rat Cerebral Artery

Oishi

Takatoh

Bao

et al. 2002

Japanese Journal of Pharmacology

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ABSTRACT-String-shaped reconstituted smooth muscle fibers were prepared in rectangular wells by thermal gelation of a mixed solution of collagen and cultured smooth muscle cells derived from the rat cerebral artery. The fibers contracted in response to KCl, 5-hydroxytryptamine (5-HT), noradrenaline, endothelin-1, endothelin-2, angiotensin II, prostaglandin F 2= and prostaglandin E 2 . 5-HT-induced contraction was partially inhibited by the L-type voltage-dependent Ca 2+ channel inhibitor nifedipine, putative non-selective cationic channel inhibitor SKF96365 and intracellular Ca 2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM), and completely abolished by the myosin light chain kinase inhibitor ML-9. The fibers pre-contracted by 5-HT were completely relaxed by the Rho kinase inhibitor Y-27632, serine /threonine kinase inhibitor staurosporine, 8-bromo cyclic GMP and papaverine, and partially relaxed by dibutyryl cyclic AMP. Moreover, 5-HT as well as endothelin-1 and KCl enhanced 20-kDa myosin light chain phosphorylation in the fibers. These results suggested that the characteristics of contraction of the fibers reflect typical contractilities of vascular smooth muscle tissues. This technique will allow us to directly address questions relating to heterogeneity of receptor mechanisms and intracellular pathways of vascular smooth muscle contraction as a function of vessel type.

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Metabolism and Roles of Eicosanoids in Brain

Farooqui

2011

Lipid Mediators and Their Metabolism in the Brain

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Cerebral vasoconstrictor mediators

Cited by 5 publications

References 133 publications

Contractile responses of smooth muscle cells differentiated from rat neural stem cells

Contractile responses of smooth muscle cells differentiated from rat neural stem cells

Contractile Responses and Myosin Phosphorylation in Reconstituted Fibers of Smooth Muscle Cells From the Rat Cerebral Artery

Metabolism and Roles of Eicosanoids in Brain

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