Cerebrospinal fluid analysis and the determination of oligoclonal bands

Gastaldi, Matteo; Zardini, Elisabetta; Leante, Rosaria; Ruggieri, Maddalena; Costa, Gianna; Cocco, Eleonora; Luca, Giovanna De; Cataldo, Ivana; Biagioli, Tiziana; Ballerini, Clara; Castellazzi, Massimiliano; Fainardi, Enrico; Pettini, P; Zaffaroni, Mauro; Giunti, Debora; Capello, Elisabetta; Bernardi, Gaetano; Ciusani, Emilio; Giannotta, Claudia; Nobile‐Orazio, Eduardo; Bazzigaluppi, Elena; Passerini, Gabriella; Bedin, Roberta; Sola, Patrizia; Brivio, Rinaldo; Cavaletti, Guido; Sala, Arianna; Bertolotto, Antonio; Desina, Gaetano; Leone, Maurizio; Mariotto, Sara; Ferrari, Sergio; Paternoster, Andreina; Giavarina, Davide; Lolli, Francesco; Franciotta, Diego

doi:10.1007/s10072-017-3034-2

Cited by 36 publications

(33 citation statements)

References 50 publications

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“…Luminescent detection, using either fluorescence or chemiluminescence, is routinely used after electrophoretic separation of proteins, mainly after sodium dodecyl sulfate (SDS) electrophoresis 11 Although chemiluminescence has been mentioned as one of the recommended techniques for IgG immunodetection in recently published guidelines on CSF analysis 12 , we were able to find few reports on luminescent detection of proteins separated by IEF as antigen-specific oligoclonal IgG (ref. [13][14][15] ) or oligoclonal IgM (ref.…”

Section: Introductionmentioning

confidence: 75%

Chemiluminescent detection of oligoclonal immunoglobulins after isoelectric focusing and affinity-mediated immunoblotting

Dlouhý¹,

Kušnierová²,

Kurasová³

et al. 2018

BIOMED PAP

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Background and Aims. Detection of oligoclonal IgG (o-IgG) in the cerebrospinal fluid (CSF) not found in serum is the principal laboratory test to support a diagnosis of multiple sclerosis. The aim of this study was to compare chemiluminescent and chromogenic detection of oligoclonal immunoglobulins in the cerebrospinal fluid and serum after their separation by means of isoelectric focusing followed by immunoblotting. Methods. A set of experiments was designed to detect oligoclonal immunoglobulins by means of alkaline phosphatase BCIP/NBT substrate and chemiluminescent peroxidase substrate. Results. Based on visual evaluation of signals, chemiluminescent detection requires about a 4 times lower amount of applied protein than very sensitive BCIP/NBT chromogenic detection. Very good correlation between methods has been shown for oligoclonal IgG. Antigen-specific oligoclonal IgG could be demonstrated by both methods although the pattern was clearer using chemiluminescence. In one patient, oligoclonal IgD bands barely visible by BCIP/NBT were convincingly demonstrated by chemiluminescence. Conclusion. Chemiluminescent detection is a feasible option for oligoclonal immunoglobulin detection and could be used in cases when the sensitivity needs to be improved. Further studies and method optimisation are warranted.

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Section: Introductionmentioning

confidence: 75%

Chemiluminescent detection of oligoclonal immunoglobulins after isoelectric focusing and affinity-mediated immunoblotting

Dlouhý¹,

Kušnierová²,

Kurasová³

et al. 2018

BIOMED PAP

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“…In 2017, cerebrospinal fluid (CSF) is done to look for oligoclonal band and immunoglobulin G (IgG), and a parallel serum sample need to be taken as no OCB production is noted in the blood in multiple sclerosis. Oligoclonal band and immunoglobulin G are indicative of intrathecal inflammation which is B cell modulated from plasma cells seen in CNS inflammatory disease [20]. Distinctive CSF analysis will disclose slightly raised leucocyte count, B cells, or plasma cells in cytological analysis and raised IgG synthesis [21].…”

Section: Lumbar Puncturementioning

confidence: 99%

Diagnosis of Multiple Sclerosis

Joseph¹

2019

Multiple Sclerosis [Working Title]

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Multiple sclerosis (MS) is a chronic inflammatory demyelinating disorder of the central nervous system. Although there are no pathognomonic features to confirm the diagnosis of this immune-mediated disease, a constellation of clinical, radiological, and immune studies can ensure the clinician gets a more definitive diagnosis. Criteria have been made every few years based on research to clinch the diagnosis. The most recent criteria which are the McDonald criteria have been formed in 2001 and revised in 2005, 2010, and 2017. Most criteria are to be used only as a guide to facilitate the diagnosis of multiple sclerosis. Advances in demyelinating disorders will require diagnostic criteria to be revised every few years with scientists hoping for a more definitive and confirmative diagnosis. The cardinal features for diagnosis are dissemination in time and space. There should be no other possible explanation to the clinical features.

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“…18 According to guidelines, a positive OCB test is defined by the presence of at least two CSFspecific bands. 19 Therefore, mixed patterns were considered to be positive when meeting this criterion. On the other hand, mirror patterns, sometimes encountered in systemic inflammatory diseases, were considered to be negative.…”

Section: Isoelectric Focusing For Detection Of Igg Oligoclonal Bandsmentioning

confidence: 99%

Free Kappa light chains in neuroinflammatory disorders: Complement rather than substitute?

2018

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Cerebrospinal fluid analysis and the determination of oligoclonal bands

Cited by 36 publications

References 50 publications

Chemiluminescent detection of oligoclonal immunoglobulins after isoelectric focusing and affinity-mediated immunoblotting

Chemiluminescent detection of oligoclonal immunoglobulins after isoelectric focusing and affinity-mediated immunoblotting

Diagnosis of Multiple Sclerosis

Free Kappa light chains in neuroinflammatory disorders: Complement rather than substitute?

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