Cerebrospinal fluid and plasma concentrations of nitric oxide metabolites in postoperative patients with subarachnoid hemorrhage

Abstract

The increase of cerebrospinal fluid nitrate after SAH may attribute to the endogenous nitric oxide production in the injured brain.

Protective Vasomotor Effects of In Vivo Recombinant Endothelial Nitric Oxide Synthase Gene Expression in a Canine Model of Cerebral Vasospasm

“…Although we have no information regarding the presence of inducible NOS (iNOS) in our animals, this would seem plausible given the association between adenoviral vectors, inflammation, and iNOS induction in vascular smooth muscle cells and infiltrating leukocytes. 15,18 Further, SAH itself is associated with inflammation and increased CSF NO levels, [17][18][19][20][21] findings supported by our data. We believe that the larger elevation of CSF NO levels observed in AdeNOS-transduced dogs is a result of expression and activity of recombinant eNOS exclusively in these animals as indicated by our immunohistochemical and Western analysis data.…”

Epidemiology and genetics of intracranial aneurysms

“…eNOS catalyses the synthesis of NO, a regulator of basal vascular tone, cerebral blood flow, and smooth-muscle cell proliferation in the arterial endothelium [59]. Several studies have shown that the NO pathway is impaired and that NO levels in cerebrospinal fluid (CSF) are raised in animals following experimentally induced SAH [58]; moreover, the NO levels have been shown to increase in humans following aneurysmal SAH [60].…”

Capillary electrophoresis analysis of biofluids with a focus on less commonly analyzed matrices

“…For example, ultrafiltered CSF was analyzed using commercial CE anion analysis reagents in studies of lupus or brain hemorrhage [142,143]. Directly injected CSF could be analyzed for nitrate and nitrite using a 100 mM borate buffer at pH 10.…”