Cerenkov counting technique

Al-Masri, M.S.

doi:10.1007/bf02036540

Cited by 19 publications

(6 citation statements)

References 23 publications

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“…Subsequently, the discs were washed in a stirring bath containing 200 ml 1% phosphoric acid, acetone and petroleum ether. The AMPK activity was counted using Cerenkov counting ( 31 ).…”

Section: Methodsmentioning

confidence: 99%

The AMPK‑mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis

Liu

Zhang

et al. 2020

Exp Ther Med

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Endometriosis is a common reproductive disorder in women, with a global prevalence of 10-15%. Long noncoding RNAs (lncRNAs) are critical to gene transcription, cell cycle modulation and immune response. The lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) reportedly mediates autophagy of endometrial stromal cells in endometriosis. The present study aimed to evaluate the role and mechanism of MALAT1 in granulosa cells (GCs) in endometriosis. Consequently, MALAT1 expression was upregulated in GCs obtained from patients with endometriosis and in the steroidogenic human granulosa-like tumor cell line KGN. However, MALAT1 knockdown consequently decreased the proliferation and viability of these cells, as determined by MTT and 5-ethynyl-2'-deoxyuridine staining assays. Both Annexin V-fluorescein isothiocyanate/propidium iodide flow cytometry and western blotting performed to detect proapoptotic factors indicated that MALAT1 depletion might promote KGN cell apoptosis. Furthermore, MALAT1 knockdown increased GC autophagy, as evidenced by microtubule-associated protein 1A/1B-light chain 3 (LC3) cleavage upregulation and p62 degradation. In addition, although 5'-AMP-activated protein kinase (AMPK) mRNA expression and protein levels decreased in GCs obtained from patients with endometriosis and KGN cells, MALAT1 knockdown restored AMPK levels. However, addition of BML-275 (MALAT1 inhibitor) to MALAT1-knockdown KGN cells recovered their viability and proliferative capacity and simultaneously reduced their apoptotic and autophagic capacity. Therefore, MALAT1 may regulate GC proliferation via AMPK-mTOR-mediated cell apoptosis and autophagy.

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“…Subsequently, the discs were washed in a stirring bath containing 200 ml 1% phosphoric acid, acetone and petroleum ether. The AMPK activity was counted using Cerenkov counting ( 31 ).…”

Section: Methodsmentioning

confidence: 99%

The AMPK‑mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis

Liu

Zhang

et al. 2020

Exp Ther Med

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“…CR is commonly known as the emergence of blue light emitted from high-energy radiation sources stored in water in a nuclear power plant. Furthermore, CR is the basic detection principle of a Cherenkov counter, which is a technique for the determination of beta emitting radionuclides used in biochemical assays (e.g., isotope 32 P) [28,29]. In general, CR can be induced when a charged particle travels faster than light in a dielectric medium (e.g., water).…”

Section: Introductionmentioning

confidence: 99%

Photopharmacological Applications for Cherenkov Radiation Generated by Clinically Used Radionuclides

Krebs

Döbber

Rodat

et al. 2021

IJMS

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Translational photopharmacological applications are limited through irradiation by light showing wavelengths within the bio-optical window. To achieve sufficient tissue penetration, using wavelengths >500 nm is mandatory. Nevertheless, the majority of photopharmacological compounds respond to irradiation with more energetic UV light, which shows only a minor depth of tissue penetration in the µm range. Thus, we became interested in UV light containing Cherenkov radiation (CR) induced as a by-product by clinically employed radionuclides labeling specific tissues. Therefore, CR may be applicable in novel photopharmacological approaches. To provide evidence for the hypothesis, we verified the clinically established radionuclides 68Ga and 90Y but not 18F in clinically used activities to be capable of generating CR in aqueous solutions. We then investigated whether the generated CR was able to photoactivate the caged kinase inhibitor cagedAZD5438 as a photoresponsive model system. Herein, 21% uncaging of the model system cagedAZD5438 occurred by incubation with 90Y, along with a non-specific compound decomposition for 68Ga and partly for 90Y. The findings suggest that the combination of a clinically employed radionuclide with an optimized photoresponsive agent could be beneficial for highly focused photopharmacological therapies.

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“…Although other examined substances have similar or higher index of refraction than methanol (methanol 1.32, water 1.33, 5 M nitric acid 1.36, PE 1.50-1.54, borosilicate glasses 1.52-1.62) and consequently lower threshold for producing of Cherenkov photons, this was not result in higher counting efficiency. The probable explanation is that out of the used solvents, methanol has the lowest density and effective proton number, which results in a longer path of the electrons with a corresponding increase in the number of photons emitted [23].…”

Section: Effects Of Sample Volume and Used Solvent On Cherenkov Detecmentioning

confidence: 99%

The effect of counting conditions on pure beta emitter determination by Cherenkov counting

Coha

Neufuss

Grahek

et al. 2016

J Radioanal Nucl Chem

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The Cherenkov counting efficiencies measured by three commercially available liquid scintillation counters, Triathler, Tri-Carb 3180, Hidex 300 SL were compared. The influences of energy of pure beta emitters, volume, and solvent as well as instrument type on Cherenkov counting efficiency were studied. The best Cherenkov counting efficiencies are on the Hidex 300 SL and when methanol is used as a solvent. It could be concluded that the sensitivities of low-level versions of Tri-Carb and Hidex 300 SL are comparable. Based on the overall results obtained it has been concluded that the TDCR Cherenkov counting is well suited for routine quantitative determination of low activities of high energetic beta emitters.

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Cerenkov counting technique

Cited by 19 publications

References 23 publications

The AMPK‑mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis

The AMPK‑mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis

Photopharmacological Applications for Cherenkov Radiation Generated by Clinically Used Radionuclides

The effect of counting conditions on pure beta emitter determination by Cherenkov counting

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