Certain Factors Affecting the Infrared Spectra of Selected Microorganisms1

Haynes, W. C.; Melvin, E. H.; Locke, Joan M.; Glass, C. A.; Senti, F. R.

doi:10.1128/aem.6.5.298-304.1958

Cited by 13 publications

(8 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7a and b. These spectra are strikingly similar to the published spectra of the material extracted from B. megaterium by Blackwood and Epp (1957) and Haynes et al (1958), and from Sphaerotilus natans by Rouf and Stokes (1962).…”

supporting

confidence: 81%

Isolation, Identification, and Metabolic Role of the Sudanophilic Granules of Zoogloea ramigera1

Crabtree

McCoy

Boyle

et al. 1965

Applied Microbiology

View full text Add to dashboard Cite

Organisms isolated from activated sludge and identified as Zoogloea ramigera accumulated large amounts of sudanophilic granules as the cultures flocculated. The granules were extracted by chloroform and precipitated with ether from acid-hydrolyzed cells. Identification of the sudanophilic granules as poly-,B-hydroxybutyric acid (PHB) was confirmed by physical, chemical, and infrared spectral analyses. The isolated polymer accounted for 12.0 to 50.5% of the dry weight of the cells. The polymer was not synthesized when the culture was grown in a growth-limiting concentration of organic substrate; it did accumulate when the culture was grown in medium enriched with carbon and energy sources. An increase in concentration of intracellular PHB was directly proportional to optical density and uptake of glucose. Aside from intracellular storage of PHB as endogenous metabolite, the accumulation of PHB is noted as a possible mechanism of flocculation.

show abstract

supporting

confidence: 81%

Isolation, Identification, and Metabolic Role of the Sudanophilic Granules of Zoogloea ramigera1

Crabtree

McCoy

Boyle

et al. 1965

Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…It melts in the range of 162 to 170 C (Williamson and Wilkinson, 1958). It has an infrared absorption spectrum similar to that of poly-f-hydroxybutyric acid isolated from Bacillus megaterium (Haynes et al, 1958). Figure 2 contains the spectra of the polybutyrate isolated from three of the strains of S. natans and, for comparison, that from B. megaterium, an organism known to form and accumulate the polymer.…”

Section: Resultsmentioning

confidence: 99%

“…It is now known that the sudanophilic granules of many genera and species of bacteria are composed mainly of poly-f-hydroxybutyric acid in association with a small amount of lipid material (Lemoigne, Delaporte, and Croson, 1944;Williamson and Wilkinson, 1958;Haynes et al, 1958;Forsyth, Hayward, and Roberts, 1958). It was of interest, therefore, to determine whether the sudanophilic granules of S. natans are also composed of poly-f-hydroxybutyric acid.…”

mentioning

confidence: 99%

ISOLATION AND IDENTIFICATION OF THE SUDANOPHILIC GRANULES OF SPHAEROTILUS NATANS

Rouf

Stokes

1962

J Bacteriol

View full text Add to dashboard Cite

The characteristic and prominent sudanophilic granules in five strains of Sphaerotilus natans were shown to be composed of poly-f-hydroxybutyric acid by the method of isolation, melting point determinations, and infrared absorption spectrum analyses. The polymer may account for 11.0 to 22.5% of the dry weight of the cells. It accumulates during active growth of S. natans and decreases sharply in amount after maximal growth. It accumulates to an equal extent in cultures with and without sheaths.

show abstract

“…(Randall et al, 1951). Since that time numerous reports have appeared describing techniques for the study of various types of bacteria by means of infrared adsorption spectra of the whole cells (Stevenson and Bolduan, 1952;Levine et al, 1953;Bailey et al, 1953;Greenstreet and Norris, 1957;and Haynes et al, 1958).…”

Section: Discussionmentioning

confidence: 99%