Certain physico-chemical properties of mosquito densonucleosis virus DNA

Buchatsky, L.

doi:10.7124/bc.000234

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…However, a weak reaction was also observed in the controls. MDV is not related serologically to the Gallena mellonella densonucleosis virus, parvoviruses of rats and pigs, enteritis of minks and Aleutian disease of minks (Buchatsky & Goral 1982, Buchatsky et al 1985. It is resistant to organic solvents (ester, chloroform), and after incubation of virus at 60°C some infectivity is still retai.ned.…”

Section: Host Rangementioning

confidence: 99%

“…As mentioned, MDV is resistant to organic solvent and different concentrations of hydrogen ions (Buchatsky et al 1985). However, MDV is sensitive to UV irradiation, even 1 min exposure results in considerable inactivation, and ca 50 % of the virus is inactivated within the first 10 min (Buchatsky & Sinitsina 1984).…”

Section: Ecology Of the Virusmentioning

confidence: 99%

“…In addition, there are particles involved in fragments of cell membranes (Buchatsky et al 1985). One Stage IV mosquito larva contained 2 pg virus, i.e.…”

Section: Host Rangementioning

confidence: 99%

“…Death rate of larvae after infection with pre-heated virus was 23 %. MDV is also highly resistant to different concentrations of hydrogen ions -it is not inactivated completely even after exposure for 24 h to acid (pH 1) or alkaline (pH 12) media (Buchatsky & Sinitsina 1984, Buchatsky et al 1985.…”

Section: Host Rangementioning

confidence: 99%

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Densonucleosis of bloodsucking mosquitoes

Buchatsky¹

1989

Dis. Aquat. Org.

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Ultrastructure, development and histopathology of a new densonucleosis vlrus of bloodsucking mosquitoes are described. Mosquito densonucleosis virus is infective to all 4 larval instars, pupae and adults of both sexes. Incubation periods were significantly shorter in older fourth-instar larvae than in third-instar larvae. Infected larvae die during the fourth larval instar or as pupae. The limited field tests conducted on this virus have shown its efficiency for infecting larvae of natural mosquito populations.

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Section: Host Rangementioning

confidence: 99%

Section: Ecology Of the Virusmentioning

confidence: 99%

“…In addition, there are particles involved in fragments of cell membranes (Buchatsky et al 1985). One Stage IV mosquito larva contained 2 pg virus, i.e.…”

Section: Host Rangementioning

confidence: 99%

Section: Host Rangementioning

confidence: 99%

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Densonucleosis of bloodsucking mosquitoes

Buchatsky¹

1989

Dis. Aquat. Org.

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Impact of different diets on the survival, pupation, and adult emergence of Culex pipiens biotype molestus larvae, and infectability with the insect-specific Culex Y virus

et al. 2023

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The current rapidly advancing climate change will affect the transmission of arthropod-borne viruses (arboviruses), mainly through changes in vector populations. Mosquitos of the Culex pipiens complex play a particularly prominent role in virus transmission in central Europe. Factors that contribute to the vector population density and the ability of those vectors to transmit viral pathogens (vector competence) can include nutrition during the larval stages. To test the influence of larval diet on larval survival and adult emergence, as well as vector competence, several diets varying in their nutritional composition were compared using a newly established assay. We tested the effects of 17 diets or diet combinations on the fitness of third-instar larvae of Culex pipiens biotype molestus. Larval survival rates at day 7 ranged from 43.33% to 94.44%. We then selected 3 of the 17 diets (Tetra Pleco, as the routine feed; JBL NovoTab, as the significantly inferior feed; and KG, as the significantly superior feed) and tested the effect of these diets, in combination with Culex Y virus infection, on larval survival rate. All Culex Y virus-infected larvae showed significantly lower larval survival, as well as low pupation and adult emergence rates. However, none of the tested diets in our study had a significant impact on larval survival in combination with viral infection. Furthermore, we were able to correlate several water quality parameters, such as phosphate, nitrate, and ammonium concentration, electrical conductivity, and low O2 saturations, with reduced larval survival. Thus, we were able to demonstrate that Culex Y virus could be a suitable agent to reduce mosquito population density by reducing larval density, pupation rate, and adult emergence rate. When combined with certain water quality parameters, these effects can be further enhanced, leading to a reduced mosquito population density, and reduce the cycle of transmission. Furthermore, we demonstrate, for the first time, the infection of larvae of the mosquito Culex pipiens biotype molestus with a viral pathogen.

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Interference between mosquito densonucleosis virus and certain arboviruses

Buchatsky,

Biletska,

Kononko

et al. 2024

J. Vet. Med. Biotechnol. Biosafety

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The active ingredient of the preparation Viroden, developed in Ukraine, is the mosquito densovirus. This virus has a wide tissue tropism and affects all phases of ontogenesis. It reproduces itself in the mosquito’s salivary gland cells, but unlike arboviruses, it is harmless for humans and vertebrates. It is well established that simultaneous infection of an insect with different viruses is often accompanied by the phenomenon of interference, whereby the reproduction of one or both viruses is suppressed in the insect’s body. Consequently, it was reasonable to investigate the results of concurrent infection with an arbovirus and a mosquito densovirus. Laboratory experiments demonstrated that mosquito densovirus suppressed the reproduction of West Nile, Sindbis, and Batai viruses in the mosquito’s body, resulting in a significant decrease in their infective titers as well as a reduction in the transmission factor during blood-feeding. The relevance of this research is determined by the increasing levels of biological threats posed by zoonotic transmissible viral infections common to humans and animals. According to the predictions of experts, in light of the processes of globalization and climate change, this may result in the emergence of new pandemics and panzootics

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Certain physico-chemical properties of mosquito densonucleosis virus DNA

Abstract: of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution//Virology.-1979.-95, N 2.-P. 476-491. 6. Reconstitution and fusogenic properties of Sendai virus envelopes/М. C. Harmsen,

Cited by 3 publications

References 16 publications

Densonucleosis of bloodsucking mosquitoes

Densonucleosis of bloodsucking mosquitoes

Impact of different diets on the survival, pupation, and adult emergence of Culex pipiens biotype molestus larvae, and infectability with the insect-specific Culex Y virus

Interference between mosquito densonucleosis virus and certain arboviruses

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