Cetylpyridinium Chloride (CPC) Treatment on Poultry Skin To Reduce Attached Salmonella

Abstract

Cetylpyridinium chloride (1-hexadecylpyridinium chloride, CPC) was evaluated for its effectiveness in removing or killing salmonellae attached to poultry skin. Two different treatment methods were used: (i) spraying 0.1% CPC solution at 15 degrees C or 50 degrees C against inoculated skin surface for I min at 138 kPa, and (ii) immersing inoculated skin surface in 0.1% CPC solution at room temperature for either 1 min, 1 min plus 2 min holding without CPC, or 3 min. After rinsing, cells on the skins were enumerated by conventional plating as well as direct counting from scanning electron microscopy (SEM). Compared with controls, CPC spraying reduced the numbers of salmonellae by 0.9 to 1.7 log units (87 to 98%) assayed by the plating method (P < 0.05). SEM gave results similar to plating. Generally 50 degrees C CPC spraying showed greater reduction than 15 degrees C CPC spraying; however, the differences were not always significant. Water spraying at either temperature did not show any reduction compared to nonsprayed skins. In the immersion test, significant differences also were noticed among the control and the three other CPC-immersed groups (P < 0.05) as assayed by plating, ranging from 1.0 to 1.6 log units, which were similar to the CPC spraying results. However, no difference was noticed among the three CPC-immersed groups. Direct counting from SEM was not a suitable method for recovering cells in CPC immersion tests because dead cells were still attached to the skin while retaining their intact morphology. On the basis of the amount of CPC used, immersion appears to be more cost-effective than spraying CPC on poultry skin.

Postharvest intervention technologies for safety enhancement of meat and meat based products; a critical review

“…Several studies report that washing poultry carcasses with 0.5 % CPC can reduce up to 2.5 log of Salmonella Typhimurium (Kim and Slavic 1996). In a study by Cutter et al (2000), they sprayed 1 % CPC solution on beef carcass and recorded E. coli O157:H7 and Salmonella Typhimurium reduction up to 5-6 log CFU/cm 2 .…”

Differential efficacy of a chlorine dioxide-containing sanitizer against single species and binary biofilms of a dairy-associated Bacillus cereus and a Pseudomonas fluorescens isolate

“…Most in vitro studies have evaluated sanitizer ef®cacy against single species bacterial bio®lms (Helke et al 1993;Ronner and Wong 1993;Brown et al 1995;Dhir and Dodd 1995;Kim and Slavik 1996;Frank and Chimielewski 1997;Lindsay and von Holy 1999). However, it has recently been reported that two bacteria present in an in vitro binary bio®lm may enhance each other's survival of sanitizer treatments compared with the corresponding single species bio®lms (Leriche and Carpentier 1995).…”

Penggunaan Cetylperidinium Chloride sebagai Anti Bakteri pada Udang

“…Senyawa ini mampu menghambat pertumbuhan L. monocytogenes pada produk-produk sayuran (Wang & Johnson, 1997;Wang & Slavik, 2001), E. coli, L. monocytogenes, S. aureus, dan S. thyphimurium pada daging sapi (Pohlman, 1999;Cutter, et al, 2000;Stopforth et al, 2002;Bosilevac et al, 2004;Lim & Mustapha, 2004;Lim & Mustapha, 2007), serta Salmonella sp. pada produk unggas (Kim & Slavik, 1996;Breen, et al, 1997).…”