Cezayir’de İnek Mastitislerinden İzole Edilen Stafilokokların Antibiyotik Direncinin Fenotipik ve Moleküler Yöntemlerle Belirlenmesi

Saidi, Radhwane; Cantekin, Zafer; Khelef, Djamel; Ergün, Yaşar; Solmaz, Hasan; Kaidi, R.

doi:10.9775/kvfd.2014.12836

Cited by 4 publications

(4 citation statements)

References 24 publications

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“…Furthermore, 41 (42.3%) and 56 (57.7%) S. aureus isolates were identified as MRSA and MSSA respectively (of the total isolated S. aureus ) that were isolated from mastitis cases based on oxacillin susceptibility testing and PCR molecular identification of the mecA that allows the discrimination between MSSA and MRSA [31]. Moreover similar to our result, a previous study in Algeria reported that, 21 Staphylococci (10 Staphylococcus aureus and 11 coagulase negative Staphylococci ) were isolated from bovine mastitis milk and these isolates were further in vitro phenotypic and genotypic resistance investigated; regarding the agar diffusion test, a total of 17 (80.95%) were identified as MRSA based on resistance to oxacillin and cefoxitin and all of them contain the mecA gene by PCR analysis similar to the present results while 76.19% were resistance to vancomycin [42] indicating (MRSA + VRSA) percentage which is more higher (76.19%) than ours (13%). On the other hand, MRSA and MSSA percentages from bovine mastitis were 2.27 and 95.45 respectively and none of the MRSA isolates were found to be resistant to vancomycin indicating (MRSA + VSSA), although it possessed both methicillin-resistant gene ( mec-A ) and vancomycin-resistant gene ( van-A ) [43] which is similar to our results.…”

Section: Discussionsupporting

confidence: 86%

MIC and MBC of Honey and Gold Nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) coagulase-positive S. aureus isolated from contagious bovine clinical mastitis

Omara

2017

Journal of Genetic Engineering and Biotechnology

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Staphylococcus aureus is one of the major causative agents of the bovine clinical mastitis. This study aimed to isolate and identify S. aureus from cases of bovine clinical mastitis followed by phenotypic detection of MRSA and VRSA. The genotypic detection of MRSA was done through PCR detection of the resistance mecA gene. Furthermore, this study aimed to investigate the in vitro MIC and MBC of the Dodonaea angustifolia plant extract, Honey, and AuNPs against the clinically isolated MRSA and VRSA. Of 93 mastitis milk samples examined, 54 (58.1%) S. aureus were isolated and identified {CP S. aureus = 46 (85.2%) and CN S. aureus = 8 (14.8%)}. The whole MRSA, VRSA, MSSA, and VSSA detected were 19 (35.2%), 7 (13%), 35 (65%), and 47 (87%) respectively. The mean counts of S. aureus were between 8.6 × 104 ± 3.5 × 105 CFU/ml. The oxacillin and vancomycin MICs against MRSA and VRSA respectively, were >256 µg/ml. AuNPs sized 30 nm produce observable in vitro anti-MRSA and anti-VRSA activities. Imtenan® citrus blossom honey has also antibacterial activities against MRSA and VRSA with general MBC and MIC range values were observed at a concentration of 0.625, 1.25, 2.5, and 5 (%v/v). In the present study, the most significant result obtained when AuNPs was mixed with Imtenan® citrus blossom honey (1:1 = v:v) with the best MBC was observed at the concentration of 0.56 × 109:0.3 (NP/ml: honey %v/v).

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Section: Discussionsupporting

confidence: 86%

MIC and MBC of Honey and Gold Nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) coagulase-positive S. aureus isolated from contagious bovine clinical mastitis

Omara

2017

Journal of Genetic Engineering and Biotechnology

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“…Similar levels of resistance to the first-line treatment with penicillin G have already been reported in strains of bovine mastitis-associated staphylococci in Algeria (Akkou et al, 2016;Saidi et al, 2015). The scientific basis for the pharmacokinetics and pharmacodynamics of antimicrobials in camels, particularly with regard to therapeutic use against mastitis, is scant (Barlow, 2011).…”

Section: Antibiotic Susceptibility Testingmentioning

confidence: 67%

Prevalence of mastitis in Algerian dromedary camels and antimicrobial resistance of the causative Staphylococci

Barka

2023

Mljekarstvo

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To investigate the prevalence, bacterial spectrum of mastitis, and antimicrobial resistance of the causative staphylococci in Algerian dromedary camels, a total of 200 lactating camels were first examined for clinical mastitis and the healthy quarters were examined for subclinical mastitis using the California Mastitis Test (CMT). Milk samples from the affected quarters were collected aseptically and analysed using conventional bacteriological isolation and identification procedures. Staphylococcal isolates were then analysed for antimicrobial resistance. The overall prevalence of mastitis in camels based on CMT and clinical examination was 35 % (70/200), of which 7.5 % (15/200) was clinical mastitis and 27.5 % (55/200) was subclinical mastitis. At the quarterly level, the overall prevalence was 11.87 % (95/800), of which 2.62 % (21/800) was clinical mastitis and 9.25 % (74/800) was subclinical mastitis. A total of 98 bacterial isolates were identified from the 95 cultured milk samples. Staphylococci (70.4%) were the predominant isolates, with 31.63 % identified as coagulase-positive staphylococci (CPS) including S. aureus at 25.51 % and 38.77 % identified as coagulase-negative staphylococci (CNS). The remaining isolates were Micrococcus sp., Streptococcus sp., Bacillus sp., E. coli, and Enterococcus. Antibiotic susceptibility testing revealed that 24 (34.78 %) of the isolates were susceptible to all antimicrobial drugs tested, while 21.73 % (15/69) were multidrug resistant strains. The highest resistance rates were found for penicillin, tetracycline and erythromycin with 33.33 %, 31.48 % and 21.74 %, respectively. Therefore, the implementation of integrated approaches in the study is of great importance for the prevention and control of mastitis to improve the quality of camel milk, minimize economic losses and avoid significant public health risks.

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“…Gram staining and catalase test [16] were performed to differentiate organisms as Staphylococci. The Staphylococci were processed to DNA extraction using bacterial DNA extraction kit (GF-1 Bacterial DNA extraction kit, Vivantis Technologies Sdn.…”

Section: Methodsmentioning

confidence: 99%

İnek Sütünde Koagulaz Pozitif Staphylococcus aureus Coa Geni Prevalansı ve İlgili Risk Faktörleri

Malik¹,

Ijaz²,

Aqib³

et al. 2017

Kafkas Univ Vet Fak Derg

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Citation of This Article AbstractThe study describes prevalence of coagulase positive Staphylococcus aureus, a zoonotic pathogen, and rule out key risk factors associated with the spread of this pathogen. A total of 900 milk samples of animals (n = 450 cattle and n = 450 buffaloes) from three subdistricts of Faisalabad were collected and processed microbiologically for staphylococci identification while, PCR for coa gene identification. Surf field mastitis test was used to screen the samples for subclinical mastitis at milking. Chi-square test was used to assess association of risk factors with mastitis. The overall prevalence of subclinical mastitis was 55% comprising 54 and 56% in cattle and buffalo's milk, respectively. A significant difference (P<0.05) was seen in the prevalence of subclinical mastitis among the cattle from different cities. The prevalence of coa gene presented 39.33% from total samples. Highest prevalence was found in tehsil Samundari followed by Jaranwala and Faisalabad presenting 43%, 39%, and 36%, respectively. The quarter based prevalence of subclinical mastitis was found 32% while 5.58% of quarters were blind. Right side quarters and teats were more affected. The risk factors except body condition and parity presented significant association with mastitis. The study concluded that bacterial coa gene, was prevalent in milk, and determinants were strongly associated with higher prevalence. Keywords: Coagulase, Staphylococcus aureus, Coa gene, Risk Factors and subdistricts İnek Sütünde Koagulaz Pozitif Staphylococcus aureus Coa Geni Prevalansı ve İlgili Risk FaktörleriÖzet Bu çalışma; zoonotik bir patojen olan koagülaz pozitif Staphylococcus aureus'un prevalansını ve bu patojenin yayılmasında rol oynayan risk fakörlerini tanımlamaktadır. Faisalabad'ın 3 ayrı bölgesindeki hayvanlardan toplanan toplam 900 adet süt örneğinde (n = 450 sığır ve n = 450 manda) Staphylococci identifikasyonu amacıyla mikrobiyolojik analiz ve coa geninin tespiti amacıyla PCR gerçekleştirildi. Subklinik mastitisin taranmasında sağım esnasında sörf mastitis testi uygulandı. Risk faktörlerinin mastitisle ilişkisini değerlendirmek amacıyla Ki-kare testi kullanıldı. Sığır ve manda sütlerinde subklinik mastitis prevalansı sırasıyla %54 ve %56'sı olarak hesaplanırken toplamda %55 olarak bulundu. Farklı şehirlerden örneklenen sığırlar arasında subklinik mastitis prevalansı yönünden anlamlı bir fark tespit edildi (P<0.05). Coa geninin prevalansı toplam örneklerde %39.33 olarak saptanmıştır. En yüksek prevalans oranı %43 olarak Samundari'de saptanırken bunu sırasıyla %39 ile Jaranwala ve %36 ile Faisalabad izlemektedir. Subklinik mastitisin meme loblarındaki prevalansı %32 olarak saptanırken meme loblarının %5.58'inde ise körelme belirlendi. Sağ taraftaki meme lobları ve meme başlarının daha fazla etkilendiği saptandı. Vücut kondisyonu ve doğum sayısı dışındaki risk faktörleri mastitis ile anlamlı ilişki içeriyordu. Çalışma, bakteriyel coa geninin sütte yaygınlığını ve göstergelerin yüksek prevalans ile kuvvetli bir ilişkis...

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Cezayir’de İnek Mastitislerinden İzole Edilen Stafilokokların Antibiyotik Direncinin Fenotipik ve Moleküler Yöntemlerle Belirlenmesi

Cited by 4 publications

References 24 publications

MIC and MBC of Honey and Gold Nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) coagulase-positive S. aureus isolated from contagious bovine clinical mastitis

MIC and MBC of Honey and Gold Nanoparticles against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) coagulase-positive S. aureus isolated from contagious bovine clinical mastitis

Prevalence of mastitis in Algerian dromedary camels and antimicrobial resistance of the causative Staphylococci

İnek Sütünde Koagulaz Pozitif Staphylococcus aureus Coa Geni Prevalansı ve İlgili Risk Faktörleri

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