CFTR is restricted to a small population of high expresser cells that provide a forskolin-sensitive transepithelial Cl– conductance in the proximal colon of the possum,<i>Trichosurus vulpecula</i>

Fan, Shujun; Harfoot, Natalie; Bartolo, Ray C.; Butt, Grant

doi:10.1242/jeb.061176

Cited by 6 publications

(2 citation statements)

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“…To study the effect of CO on CFTRmediated chloride (Cl -) secretion, forskolin, an adenylate cyclase activator, was used to induce cAMP/PKA signalling [21]. The forskolin-induced increase in cAMP and subsequent increase in I SC was inhibited by CORM-3 (Fig.…”

Section: Inhibitory Effect Of Corms On Camp-dependent I Scmentioning

confidence: 99%

Carbon Monoxide Inhibits Cytokine and Chloride Secretion in Human Bronchial Epithelia

Zhang

Yip

2018

Cell Physiol Biochem

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Background/Aims: Carbon monoxide (CO) is an important gas produced endogenously by heme oxygenase (HO) that functions as an anti-inflammatory and in ion channel modulation, but the effects of CO on airway inflammation and ion transport remains unclear. Methods: The effect of CO on cell damage- and nucleotide-induced pro-inflammatory cytokine release in primary human bronchial epithelia cells (HBE) and in the 16HBE14o- human bronchial epithelial cell line were investigated. The effects of CO on calcium- and cAMP-dependent chloride (Cl^-) secretion were examined using a technique that allowed the simultaneous measurement and quantification of real-time changes in signalling molecules (cAMP and Ca²⁺) and ion transport in a polarised epithelium. Results: CO suppressed the release of interleukin (IL)-6 and IL-8 and decreased the phosphorylation of ERK1/2 and NF-κB p65. Furthermore, CO inhibited UTP-induced increases in calcium and Cl^- secretion, and forskolin-induced increases in cAMP and Cl^- secretion. Conclusions: These findings suggest a novel anti-inflammatory role of CO in human bronchial epithelia via interactions with purinergic signalling pathways. Further, CO modulated both the Ca²⁺- and cAMP-dependent secretion of Cl^-.

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Section: Inhibitory Effect Of Corms On Camp-dependent I Scmentioning

confidence: 99%

Carbon Monoxide Inhibits Cytokine and Chloride Secretion in Human Bronchial Epithelia

Zhang

Yip

2018

Cell Physiol Biochem

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“…Second, and more importantly for maintaining ASL, unlike large airways, bronchioles lack submucosal glands as a source for secreting and replenishing surface fluid. How small airways meet the challenge of maintaining just the "right" depth of ASL in vivo is not established, but perhaps because the airway microanatomical surface is usually conceptualized as a flat epithelium (91), it has been widely accepted that the luminal epithelial cells alternate in unison between uniformly absorbing fluid when ASL is excessive and uniformly secreting fluid when ASL is depleted in a manner that might be seen as a "drain and flood" model (15,16,21,32) (see Supplemental Figure S1 at Physiology website).…”

Section: The Airway Surface Liquid Challengementioning

confidence: 99%

Both Ways at Once: Keeping Small Airways Clean

Quinton

2017

Physiology

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The small airways of the lungs are under constant assault from the pathogens and debris in the air that they must conduct to alveoli. Although hygiene is of paramount importance for respiratory health, the underlying principles of airway clearance have not been well integrated or established. Newly emerging concepts of simultaneous absorption and secretion of airway surface liquid (ASL) and the role of [Formula: see text] in the maturation of mucins have advanced from experimental evidence as well as observations from the congenital disease cystic fibrosis (CF) to present a novel model that integrates microanatomy with organ physiology to meet the constant challenge of cleaning small airways.

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Physiologic Influences of Transepithelial K+ Secretion

Halm

2015

Ion Channels and Transporters of Epithelia in Health and Disease

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CFTR is restricted to a small population of high expresser cells that provide a forskolin-sensitive transepithelial Cl– conductance in the proximal colon of the possum,Trichosurus vulpecula

Cited by 6 publications

References 50 publications

Carbon Monoxide Inhibits Cytokine and Chloride Secretion in Human Bronchial Epithelia

Carbon Monoxide Inhibits Cytokine and Chloride Secretion in Human Bronchial Epithelia

Both Ways at Once: Keeping Small Airways Clean

Physiologic Influences of Transepithelial K+ Secretion

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