CFU-S11 activity does not localize solely with the aorta in the aorta-gonad-mesonephros region

Bruijn, Marella F. T. R. de; Peeters, Maria C. W.; Luteijn, Tanya; Visser, Pim; Speck, Nancy A.; Dzierzak, Elaine

doi:10.1182/blood.v96.8.2902.h8002902_2902_2904

Cited by 8 publications

(8 citation statements)

References 13 publications

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“…CFUs precursors were present in both AGM and YS by E9 since cultures of either tissue at this stage generated CFU-S (42). In vivo engraftment was obtained with cells from the E10.5 dorsal aorta and vitelline/umbilical artery (80,81). Quantitation of long-term repopulating stem cell numbers by limiting dilution assay has shown that they first appear in the AGM at E10.5 and are present in equal numbers in AGM, YS, circulation, and early liver rudiment by E11 (43).…”

Section: Development Of Hematopoiesis In the Aorta-gonad Mesonephros mentioning

confidence: 99%

Commentary: The Role of Cell Migration in the Ontogeny of the Lymphoid System

Moore

2004

Stem Cells and Development

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The foundations of experimental hematology were laid by histologists, and while their contributions were enormous, they were limited in their interpretation of very dynamic processes by the static nature of the methodology. The middle of the twentieth century saw the introduction of techniques for hematopoietic cell marking and development of in vitro and in vivo assays for primitive hematopoietic cells, allowing dynamic studies of hematopoiesis. Paralleling this was an understanding of cellular immunology with the discovery of the role of the thymus and the identification of T and B lymphocyte lineages. In the 1960s a series of ontogenetic studies in birds and subsequently in mice revealed that hematopoietic and lymphoid development involved migration streams of primitive cells that colonized developing primary lymphoid organs as well as spleen, marrow, and liver. The yolk sac was proposed as the ultimate origin of these lympho-hematopoietic precursors. Subsequent studies identified a region associated with the dorsal aorta as the primary site of "definitive" stem cells. These opposing views are currently achieving a compromise that recognizes that both sites contribute stem cells involved in seeding the developing tissues. The clear distinction between the local origin of the inducing microenvironment provided by the endoderm or by stroma derived from mesenchymal stem cells of mesodermal origin, and the immigrant origin of the hematopoietic stem cells and progenitors, raises intriguing questions in the current climate of stem cell plasticity, cell fusion, and discovery of stem cells in adult marrow with the capacity to generate hematopoiesis as well as other mesodermal, ectodermal, and endodermal lineages.

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Section: Development Of Hematopoiesis In the Aorta-gonad Mesonephros mentioning

confidence: 99%

Commentary: The Role of Cell Migration in the Ontogeny of the Lymphoid System

Moore

2004

Stem Cells and Development

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“…1C). The AGM region was subdissected using 27G needles into the aorta, with surrounding mesenchymal tissue (AM), and the urogenital ridges, containing the pro/mesonephros and the gonads (UG) (de Bruijn et al, 2000a;de Bruijn et al, 2000b). Subdissected tissues from litters with at least seven embryos were pooled and either explant cultured at the air-medium interface on 0.1% gelatin-coated 6-well plates (Costar, Badhoevedorp, NL) or cultured as a single cell suspension (after a 15 minute incubation with 0.25% trypsin and vigorous pipetting) on 0.1% gelatin-coated 6-well plates in stromal medium: 50% long-term culture medium (M5300, StemCell Technologies, Vancouver, BC, Canada), 15% FCS, 35% AlphaMEM, supplemented with antibiotics (penicillin and streptomycin; Gibco), Glutamax-I (Gibco) and 10 µM β-mercaptothanol (Merck Eurolab, Darmstadt, Germany).…”

Section: Transgene and Expression Analysismentioning

confidence: 99%

Embryonal subregion-derived stromal cell lines from novel temperature-sensitive SV40 T antigen transgenic mice support hematopoiesis

Oostendorp

Medvinsky

Kuşadasi

et al. 2002

Journal of Cell Science

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Throughout life, the hematopoietic system requires a supportive microenvironment that allows for the maintenance and differentiation of hematopoietic stem cells (HSC). To understand the cellular interactions and molecules that provide these functions, investigators have previously established stromal cell lines from the late gestational stage and adult murine hematopoietic microenvironments. However, the stromal cell microenvironment that supports the emergence, expansion and maintenance of HSCs during mid-gestational stages has been largely unexplored. Since several tissues within the mouse embryo are known to harbor HSCs (i.e. aortagonads-mesonephros, yolk sac, liver), we generated numerous stromal cell clones from these mid-gestational sites. Owing to the limited cell numbers,isolations were performed with tissues from transgenic embryos containing the ts SV40 Tag gene (tsA58) under the transcriptional control of constitutive and ubiquitously expressing promoters. We report here that the growth and cloning efficiency of embryonic cells (with the exception of the aorta) is increased in the presence of the tsA58 transgene. Furthermore, our results show that the large panel of stromal clones isolated from the different embryonal subregions exhibit heterogeneity in their ability to promote murine and human hematopoietic differentiation. Despite our findings of heterogeneity in hematopoietic growth factor gene expression profiles, high-level expression of some factors may influence hematopoietic differentiation. Interestingly, a few of these stromal clones express a recently described chordin-like protein, which is an inhibitor of bone morphogenic proteins and is preferentially expressed in cells of the mesenchymal lineage.

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“…The generation of the first transplantable hematopoietic stem cells (HSCs) initiates in the dorsal aorta of the aortagonad-mesonephros (AGM) region at embryonic day 10.5 (E10.5) in mouse embryos (de Bruijn et al, 2000;Medvinsky and Dzierzak, 1996). It involves activating a hematopoietic transcriptional program in a subset of endothelial cells (ECs), as a result of a number of internal and external signals, which drives these cells to adopting a hematopoietic fate (Ottersbach, 2019).…”

Section: Introductionmentioning

confidence: 99%