CFU-S11 activity does not localize solely with the aorta in the aorta-gonad-mesonephros region

Bruijn, Marella F. T. R. de; Peeters, Maria C. W.; Luteijn, Tanya; Visser, Pim; Speck, Nancy A.; Dzierzak, Elaine

doi:10.1182/blood.v96.8.2902

Cited by 24 publications

(7 citation statements)

References 13 publications

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“…the HSCs are localized in the dorsal aorta, but by 12 d.p.c. they can also be found in the urogenital system, which develops in close proximity to the dorsal aorta, as noted in (21) (see Fig. 1).…”

Section: Hscs In the Intra‐embryonic Agm Regionsupporting

confidence: 58%

The embryonic aorta‐gonad‐mesonephros region as a generator of haematopoietic stem cells

Pietilä

Vainio

2005

APMIS

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During mammalian embryonic development the definitive haematopoietic stem cells (HSCs) may arise either in the extra-embryonic mesoderm or in the aorta-gonad-mesonephros (AGM) region that forms in close proximity to the assembling urogenital system, generating the gonad, cortex of the adrenal gland and metanephros. Researchers have been attempting for a long time to define the region of importance for generating the definitive HSCs that colonize the fetal liver and bone marrow, the two major sites where haematopoiesis takes place in the adult. The fetal liver might gain HSCs from both of the primary haematopoietic sources, but the extra-embryonic HSCs seem not to be able to colonize adult bone marrow directly. It is known that the microenvironment around the HSCs is important for directing cell fates, but we do not yet have much idea about the cell-cell interactions, tissue interactions and molecules that regulate cell behaviour in the AGM. We will here discuss the contribution of the AGM to definitive haematopoiesis in mammals and review some of the cell-cell interactions and associated signalling systems involved in the development of AGM stem cells.

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Section: Hscs In the Intra‐embryonic Agm Regionsupporting

confidence: 58%

The embryonic aorta‐gonad‐mesonephros region as a generator of haematopoietic stem cells

Pietilä

Vainio

2005

APMIS

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“…6D, 6E). The vitelline and umbilical arteries are analogous to dorsal aorta in that they are major vasculatures in nature and can autonomously generate HSC almost concomitantly [27,28]. However, we could not detect any HPP-HA after testing 65 HPP colonies in three independent experiments (Fig.…”

Section: Hpp-ha Was Not Detected Within Yolk Sac Even Cocultured Withmentioning

confidence: 88%

Identification of High Proliferative Potential Precursors with Hemangioblastic Activity in the Mouse Aorta-Gonad- Mesonephros Region

Yao¹,

Liu²,

Wang³

et al. 2007

Stem Cells

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Hemangioblast, a precursor possessing hematopoietic and endothelial potential, is identified as the blast colony-forming cell in the murine gastrulating embryos (E7.0 -E7.5). Whether hemangioblast exists in the somite-stage embryos is unknown, even though hemogenic endothelium is regarded as the precursor of definitive hematopoiesis in the aortagonad-mesonephros (AGM) region. To address the issue, we developed a unique three-step assay of high proliferative potential (HPP) precursors. The AGM region contained a kind of HPP precursor that displayed hematopoietic selfrenewal capacity and was able to differentiate into functional endothelial cells in vitro (i.e., incorporating DiI-acetylated low-density lipoprotein, expressing von Willebrand factors, and forming network structures in Matrigel). The clonal nature was verified by cell mixing assay. However, the bilineage precursor with high proliferative potential-the HPP-hemangioblast (HA)-was not readily detected in the yolk sac (E8.25-E12.5), embryonic circulation (E10.5), placenta (E10.5-E11.5), fetal liver (E11.5-E12.5), and even umbilical artery (E11.5), reflective of its strictly spatial-regulated ontogeny. Expression of CD45, a panhematopoietic marker, distinguished hematopoietic-restricted HPP-colony-forming cell from the bipotential HPP-HA. Finally, we revealed that basic fibroblast growth factor, other than vascular endothelial growth factor or transforming growth factor-␤1, was a positive modulator of the HPP-HA proliferation. Taken together, the HPP-HA represents a novel model for definitive hemangioblast in the mouse AGM region and will shed light on molecular mechanisms underlying the hemangioblast development.

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“…Long‐term multilineage repopulation, CFU‐S, and culture CFU (CFU‐C) activity were determined as described [14, 18, 19] with minor modifications. BM cell populations were assayed for HSC activity by intravenous transfer into irradiated (900 rad, split dose) 3‐ to 6‐month‐old (129S3/SvImJ × C57BL/6J) F1 recipients.…”

Section: Methodsmentioning

confidence: 99%

Runx1 Is Expressed in Adult Mouse Hematopoietic Stem Cells and Differentiating Myeloid and Lymphoid Cells, But Not in Maturing Erythroid Cells

et al. 2004

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CFU-S11 activity does not localize solely with the aorta in the aorta-gonad-mesonephros region

Cited by 24 publications

References 13 publications

The embryonic aorta‐gonad‐mesonephros region as a generator of haematopoietic stem cells

The embryonic aorta‐gonad‐mesonephros region as a generator of haematopoietic stem cells

Identification of High Proliferative Potential Precursors with Hemangioblastic Activity in the Mouse Aorta-Gonad- Mesonephros Region

Runx1 Is Expressed in Adult Mouse Hematopoietic Stem Cells and Differentiating Myeloid and Lymphoid Cells, But Not in Maturing Erythroid Cells

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