Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges

Rossi, Luis Francisco; Luaces, Juan Pablo; Browne, Melanie; Chirino, Mónica Gabriela; Merani, María Susana; Mudry, Marta Dolores

doi:10.1016/j.mrgentox.2015.11.008

Cited by 9 publications

(12 citation statements)

References 55 publications

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“…No significant differences were between the sexes with respect to the studied biomarkers: CA: 0.23 ± 0.01and 0.23 ± 0.01 for males and females respectively ( p > 0.99); SCE: 6.64 ± 0.21 and 6.69 ± 0.16 for males and females respectively ( p > 0.98); RI: 1.63 ± 0.01 and 1.63 ± 0.02 for males and females respectively ( p > 0.97). The values obtained for the negative controls are in line with basal values reported in previous work [ 20 ] ( Table 1 ; Fig 1A–1C ).…”

Section: Resultssupporting

confidence: 91%

“…The present work examines the genotoxic and cytogenetic effects of RU on the armadillo C . villosus , which has recently been established as a sentinel species [ 20 ], via the analysis of CAs, SCEs and the calculation of the RI. Since in the 1120 RU treatment the cells failed to grow, no analyses were made for this concentration.…”

Section: Resultsmentioning

confidence: 99%

“…Chetophractus villosus was recently established as a sentinel species for testing potential genotoxicity [ 20 ]. These semi-fossorial armadillos are exposed to different substances in the soils.…”

Section: Resultsmentioning

confidence: 99%

“…Cultures without glyphosate served as negative controls; positive controls were supplied by cells cultured in the presence of mitomycin C (MMC) (8.97 μmol/L). All experiments were performed after first exposing the cultures to 1 mg/mL 5-bromo-2'-deoxyuridine (BrdU) to assist the viewing of SCE [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…We recently described baseline values for the mitotic index (MI), the percentage of cells with chromosome aberrations (CAs), and the mean number of sister chromatid exchanges (SCEs) per cell in C . villosus captured in pristine areas (i.e., where no agrochemical agents are applied) [ 20 ]. Chromosome damage has been used in some ecotoxicological biomonitoring studies as a means of characterizing the genotoxic effects of chemical agents [ 21 , 22 ], with CAs and SCEs commonly used as cytogenetic endpoints in hazard identification assays [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Genotoxic effects of Roundup Full II® on lymphocytes of Chaetophractus villosus (Xenarthra, Mammalia): In vitro studies

et al. 2017

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In Argentina, Chaetophractus villosus has a wide distribution that overlaps with agricultural areas where soybean is the predominant crop. In such areas the pesticide Roundup Full II® (RU) is widely applied. The genotoxic effect of its active ingredient glyphosate (RU is 66.2% glyphosate) on the peripheral blood lymphocytes of C. villosus was tested over a range of concentrations (280, 420, 560, 1120 μmol/L). Culture medium without glyphosate served as negative control, while medium containing mitomycin C served as positive control. Genetic damage was characterized in terms of the percentage of cells with chromosome aberrations (CA), the mean number of sister chromatid exchanges (SCE) per cell, and the modification of cell proliferation kinetics via the calculation of the replication index. Significant increases (p < 0.0001) were seen in the CA frequency and the mean number of SCEs per cell compared to negative controls at all the RU concentrations tested. Chromatid breaks, the only form of CA observed, under the 560 μmol/L RU conditions and in presence of mitomycin C were four to five times more common than at lower concentrations, while no viable cells were seen in the 1120 μmol/L treatment. The mean number of SCEs per cell was significantly higher under the 280 μmol/L RU conditions than the 420 or 560 μmol/L RU conditions; cells cultivated in the presence of MMC also showed significantly more SCEs. All the RU concentrations tested (except in the 1120 μmol/L RU treatment [no viable cells]) induced a significant reduction in the replication index (p < 0.0001). The present results confirm the genotoxic effects of RU on C. villosus lymphocytes in vitro, strongly suggesting that exposure to RU could induce DNA damage in C. villosus wildlife.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%