Chalcone isomerase-like genes in Tradescantia BNL4430: identification, molecular characterization, and differential expression profiles under Ɣ-radiation stress

Subburaj, Saminathan; Ha, Hye-Jeong; Park, Nuri; Choi, Seo-Hee; Lee, Geung–Joo

doi:10.1007/s13562-017-0396-8

Cited by 3 publications

(1 citation statement)

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“…For RT-qPCR, a 20 µL reaction volume containing 2 µL of reverse-transcribed cDNA, 2× quantispeed SYBR ® Green mix (PhileKorea, Daejeon, Korea), and 0.5 µM of gene-specific forward and reverse primers were used to calculate the mRNA expression level of TrCNX using the Eco TM Real Time PCR System (Illumina, Seoul, Republic of Korea). The RT-qPCR was performed according to our previous study in Tradescantia [42]. Using three biological replicates for each gene in the PCR, the relative expression levels of TrCNX-1 were normalized according to that of the reference gene, actin-7, using the 2 -∆∆CT method [43].…”

Section: Mrna Isolation C-dna Cloning Rt-pcr and Rt-qpcr Analysismentioning

confidence: 99%

Molecular Characterization and Identification of Calnexin 1 As a Radiation Biomarker from Tradescantia BNL4430

Subburaj

Kim

et al. 2020

Plants

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Calnexin (CNX) is an integral membrane protein that functions as a chaperone in the endoplasmic reticulum for the correct folding of proteins under stress conditions, rendering organisms tolerant under adverse conditions. Studies have investigated the cytogenetic effects of gamma irradiation (Ɣ-IR) on plants, but information on the molecular response under Ɣ-IR remains limited. Previously, we constructed a cDNA library of an irradiation-sensitive bioindicator plant, Tradescantia BNL4430 (T-4430) under Ɣ-IR, in which the Calnexin-1 gene was highly upregulated at 50 mGy treatment. TrCNX1 encodes a 61.4 kDa protein with conserved signature motifs similar to already reported CNX1s. TrCNX1 expression was evaluated by semiquantitative reverse transcriptase PCR and quantitative real-time PCR and was ubiquitously expressed in various tissues and highly upregulated in flower petals under 50 mGy Ɣ-IR stress. The protective function of TrCNX1 was investigated by overexpression of TrCNX1 in an Escherichia coli BL21(DE3) heterologous system. Using plate assay, we showed that TrCNX1 increased the viability of E. coli transformants under both UV-B and Ɣ-IR compared with the control, demonstrating that TrCNX1 functions under irradiation stress. TrCNX1 may enhance irradiation stress tolerance in crops and act as a radio marker gene to monitor the effects of radiation.

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Section: Mrna Isolation C-dna Cloning Rt-pcr and Rt-qpcr Analysismentioning

confidence: 99%