Challenges associated with spray drying of lactic acid bacteria: Understanding cell viability loss

Moreira, Maria Tereza Cratiú; Martins, Evandro; Perrone, Ítalo Tuler; Freitas, Renato de; Queiroz, Lucas Sales; Carvalho, Antônio Fernandes de

doi:10.1111/1541-4337.12774

Cited by 31 publications

(11 citation statements)

References 179 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in the SD method, drying occurs when a continuous liquid film is converted into droplets followed by exposure to a hot, dry airflow. The increase in heat exchange area with a high-temperature difference enables to speed up the drying process ( Moreira et al., 2021 ). However, the heat exchange process can have a direct damaging effect on thermosensitive phages affecting their viability.…”

Section: Inhaled Phage Therapy: a New Era Of Therapeuticsmentioning

confidence: 99%

Prospects of Inhaled Phage Therapy for Combatting Pulmonary Infections

Wang

Xie

Zhao

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

With respiratory infections accounting for significant morbidity and mortality, the issue of antibiotic resistance has added to the gravity of the situation. Treatment of pulmonary infections (bacterial pneumonia, cystic fibrosis-associated bacterial infections, tuberculosis) is more challenging with the involvement of multi-drug resistant bacterial strains, which act as etiological agents. Furthermore, with the dearth of new antibiotics available and old antibiotics losing efficacy, it is prudent to switch to non-antibiotic approaches to fight this battle. Phage therapy represents one such approach that has proven effective against a range of bacterial pathogens including drug resistant strains. Inhaled phage therapy encompasses the use of stable phage preparations given via aerosol delivery. This therapy can be used as an adjunct treatment option in both prophylactic and therapeutic modes. In the present review, we first highlight the role and action of phages against pulmonary pathogens, followed by delineating the different methods of delivery of inhaled phage therapy with evidence of success. The review aims to focus on recent advances and developments in improving the final success and outcome of pulmonary phage therapy. It details the use of electrospray for targeted delivery, advances in nebulization techniques, individualized controlled inhalation with software control, and liposome-encapsulated nebulized phages to take pulmonary phage delivery to the next level. The review expands knowledge on the pulmonary delivery of phages and the advances that have been made for improved outcomes in the treatment of respiratory infections.

show abstract

Section: Inhaled Phage Therapy: a New Era Of Therapeuticsmentioning

confidence: 99%

Prospects of Inhaled Phage Therapy for Combatting Pulmonary Infections

Wang

Xie

Zhao

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Spray drying has been used for the production of starter cultures and dehydrated lactic acid bacteria, as the powder obtained can be transported at a low cost and can be stored in a stable form for prolonged periods [ 29 ]. The cell viability of individual bacterium may be affected by various factors during the spray-drying process, which occur over a very brief period of time, which makes it difficult to predict the full damage that bacteria cultures incur throughout the drying process [ 30 ]. In addition, parameters such as air flow, mechanical processing, storage conditions, and encapsulated material can influence the survivability of bacteria [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Influence of Fermentation Beetroot Juice Process on the Physico-Chemical Properties of Spray Dried Powder

et al. 2022

View full text Add to dashboard Cite

Picking vegetables is, along with salting and drying, one of the oldest ways to preserve food in the world. This is the process of decomposition of simple sugars into lactic acid with the participation of lactic bacteria. The aim of the study was to obtain powders from fermented red beet juice with the highest possible amount of lactic acid bacteria (LAB) and active ingredients. For the analysis, juices were squeezed from the vegetables and two types of fermentation were used: a spontaneous fermentation and a dedicated one. After inoculation, samples were taken for analysis on a daily basis. Extract, pH, total acidity, pigments, and color were measured. In addition, microbiological tests were also carried out. The juices from the fifth day of fermentation was also spray dried, to obtain fermented beetroot powder. Juices from 3–5th day were characterized by a high content of LAB and betanin, had also a low pH, which proves that the lactic fermentation is working properly. The exception was the juice from spontaneous fermentation. According to the observations, the fermentation process did not run properly, and further analysis is needed. The powders were stable; however, results obtained from the pigment content and the LAB content are not satisfactory and require further analysis.

show abstract

“…Dairy Science and Technology, 2. PAPER 1 Viana, C. C. R., Renhe, I. R. T., de Bessa, M. E., Martins, E., Stephani, R., de Carvalho, A. F., & Perrone, Í. T. (2021). Microencapsulamento de bactérias probióticas: uma breve revisão.…”

Section: Referencesunclassified

“…The spray drying technique consists basically of atomization of the material to be dried to form small droplets (10 µm to 150 µm) which are dispersed into a hot and dry air flow (Dolinsky, 2001;Thybo et al, 2008). Due to the small size of the droplets and the high difference between the temperature and water content of the droplet and air, the water is removed by evaporation almost instantly without overheating of the material (around 45°C) (Haque & Adhikari, 2015;Perrone et al, 2016;Moreira et al, 2021). This makes it possible to produce dried LAB cultures even using air drying with high temperatures (between 140°C and 200°C) .…”

Section: Principle Of Dehydration By Lyophilization and Spray Dryingmentioning

confidence: 99%

“…flexibility in controlling drying properties; the possibility of large-scale production and continuous operation, even with simple equipment; the production of relatively uniform and spherical particles; good process efficiency and low cost; better storage stability compared to cultures kept frozen or fresh cultures and the possibility of storage at room temperature Martins et al, 2017;.According to Moreira et al, (2021) the spray dryer technique can be potentially used for the production of dehydrated LAB cultures, and has been researched for applications in new products in several areas, including food.…”

Section: Principle Of Dehydration By Lyophilization and Spray Dryingmentioning

confidence: 99%

See 1 more Smart Citation

Study of the bacteria - encapsulating agent interaction in microencapsulation of probiotics

Viana¹

View full text Add to dashboard Cite

An increasing number of microorganisms have been applied in the food industry, by incorporation in food products, in the development of probiotic ingredients and supplements, among others. This industrial use usually depends on preservation methods through dehydration, in order to ensure better metabolic activity. On the other hand, drying can cause loss of viability mainly due to cellular damage caused by this process. To avoid them, we can mention the use of microencapsulation, an important technique to improve bacterial stability during processing and application in food matrices. Thus, the objective of this thesis was to review the scientific literature on the microencapsulation of microorganisms, with a main focus on probiotics and the main encapsulating agents used by the lyophilization and spray dryer method. In this scenario, lactic acid bacteria stand out, which have been increasingly chosen in research and applications in food, especially in dairy products. Thus, in the first work, already published, a general review was carried out on microencapsulation of probiotic bacteria, focusing on spray drying and the main encapsulating agents cited in the literature for this encapsulation method. It was observed that in the literature the authors increasingly seek to optimize the production of dehydrated microbial cultures, which makes it necessary to know about the encapsulating materials and the main available and applicable methods. At the same time, they cite the need for more research, mainly to understand the protective effect, in order to guide the choice of conditions to be used in microencapsulation. Therefore, the second work brings a denser review of the literature, having as main focus the microencapsulation of lactic acid bacteria by lyophilization and spray dryer. In this article, the main hypotheses found in the literature about the negative effect of bacterial dehydration are discussed, as well as the protective action of carbohydrates, proteins and lipids used as encapsulating agents. To investigate the theories discussed, physicochemical analytical methods are suggested, in order to aid in the understanding of microencapsulation, together with microbiological methods already commonly used.Keywords: Microorganisms. Food. Viability. Drying. Spray dryer.

show abstract

Challenges associated with spray drying of lactic acid bacteria: Understanding cell viability loss

Cited by 31 publications

References 179 publications

Prospects of Inhaled Phage Therapy for Combatting Pulmonary Infections

Prospects of Inhaled Phage Therapy for Combatting Pulmonary Infections

Influence of Fermentation Beetroot Juice Process on the Physico-Chemical Properties of Spray Dried Powder

Study of the bacteria - encapsulating agent interaction in microencapsulation of probiotics

Contact Info

Product

Resources

About