Challenges in the development of egg-independent vaccines for influenza

Trombetta, Claudia Maria; Marchi, Serena; Manini, Ilaria; Lazzeri, Giacomo; Montomoli, Emanuele

doi:10.1080/14760584.2019.1639503

Cited by 35 publications

(32 citation statements)

References 160 publications

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“…Another important consideration is the fact that the currently available influenza vaccines are not able to protect against emerging pandemic-like influenza viruses [11]. Moreover, with today's manufacturing technologies, it would take at least six-to-eight months to prepare a new vaccine; in the event of urgent necessity, this may be too long, as demonstrated by the 2009 H1N1 pandemic [12,13].…”

Section: Introductionmentioning

confidence: 99%

Influenza Anti-Stalk Antibodies: Development of a New Method for the Evaluation of the Immune Responses to Universal Vaccine

et al. 2020

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Growing interest in universal influenza vaccines and novel administration routes has led to the development of alternative serological assays that are able to detect antibodies against conserved epitopes. We present a competitive ELISA method that is able to accurately determine the ratio of serum immunoglobulin G directed against the different domains of the hemagglutinin, the head and the stalk. Human serum samples were treated with two variants of the hemagglutinin protein from the A/California/7/2009 influenza virus. The signals detected were assigned to different groups of antibodies and presented as a ratio between head and stalk domains. A subset of selected sera was also tested by hemagglutination inhibition, single radial hemolysis, microneutralization, and enzyme-linked lectin assays. Pre-vaccination samples from adults showed a quite high presence of anti-stalk antibodies, and the results were substantially in line with those of the classical serological assays. By contrast, pre-vaccination samples from children did not present anti-stalk antibodies, and the majority of the anti-hemagglutinin antibodies that were detected after vaccination were directed against the head domain. The presented approach, when supported by further assays, can be used to assess the presence of specific anti-stalk antibodies and the potential boost of broadly protective antibodies, especially in the case of novel universal influenza vaccine approaches.

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Section: Introductionmentioning

confidence: 99%

Influenza Anti-Stalk Antibodies: Development of a New Method for the Evaluation of the Immune Responses to Universal Vaccine

et al. 2020

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“…The majority of the global production of influenza vaccine is currently performed in embryonated hen's eggs [1] with a growing proportion in mammalian cell lines such as Madin-Darby canine kidney cells (MDCK) [2]. Both of these methods derive their initial viruses from clinical respiratory samples of patients with influenza-like illness (ILI), which are inoculated directly into these substrates [3].…”

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confidence: 99%

SARS-CoV-2 does not replicate in embryonated hen’s eggs or in MDCK cell lines

et al. 2020

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The advent of COVID-19, has posed a risk that human respiratory samples containing human influenza viruses may also contain SARS-CoV-2. This potential risk may lead to SARS-CoV-2 contaminating conventional influenza vaccine production platforms as respiratory samples are used to directly inoculate embryonated hen’s eggs and continuous cell lines that are used to isolate and produce influenza vaccines. We investigated the ability of these substrates to propagate SARS-CoV-2 and found that neither could support SARS-CoV-2 replication.

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“…For instance, an SRMA by Liao et al [76] found that a booster did not have any beneficial effect on the immune response in patients on hemodialysis or peritoneal dialysis or in renal transplant recipients. Moreover, the novel egg-independent technologies with several potential advantages are becoming increasingly common [77,78] and should be further investigated also from the immunogenicity point of view.…”

Section: Discussionmentioning

confidence: 99%

Mapping Host-Related Correlates of Influenza Vaccine-Induced Immune Response: An Umbrella Review of the Available Systematic Reviews and Meta-Analyses

et al. 2019

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Seasonal influenza is the leading infectious disease in terms of its health and socioeconomic impact. Annual immunization is the most efficient way to reduce this burden. Several correlates of influenza vaccine-induced protection are commonly used, owing to their ready availability and cheapness. Influenza vaccine-induced immunogenicity is a function of host-, virus-and vaccine-related factors. Host-related factors constitute the most heterogeneous group. The objective of this study was to analyze the available systematic evidence on the host factors able to modify influenza vaccine-induced immunogenicity. An umbrella review approach was undertaken. A total of 28 systematic reviews/meta-analyses were analyzed-these covered the following domains: intravenous drug use, psychological stress, acute and chronic physical exercise, genetic polymorphisms, use of pre-/pro-/symbiotics, previous Bacillus Calmette-Guérin vaccination, diabetes mellitus, vitamin D supplementation/deficiency, latent cytomegalovirus infection and various forms of immunosuppression. In order to present effect sizes on the same scale, all possible meta-analyses were re-performed and cumulative evidence synthesis ranking was carried out. The meta-analysis was conducted separately on each health condition category and virus (sub)type. A total of 97 pooled estimates were used in order to construct an evidence-based stakeholder-friendly map. The principal public health implications are discussed.

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Challenges in the development of egg-independent vaccines for influenza

Cited by 35 publications

References 160 publications

Influenza Anti-Stalk Antibodies: Development of a New Method for the Evaluation of the Immune Responses to Universal Vaccine

Influenza Anti-Stalk Antibodies: Development of a New Method for the Evaluation of the Immune Responses to Universal Vaccine

SARS-CoV-2 does not replicate in embryonated hen’s eggs or in MDCK cell lines

Mapping Host-Related Correlates of Influenza Vaccine-Induced Immune Response: An Umbrella Review of the Available Systematic Reviews and Meta-Analyses

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