Challenges in the identification of species of canned fish

Mackie, I. M.; Pryde, Susan E.; Gonzales-Sotelo, Carmen; Medina, Isabel; Pérez‐Martín, Ricardo I.; Quinteiro, Javier; Rey-Méndez, Manuel; Rehbein, Hartmut

doi:10.1016/s0924-2244(99)00013-8

Cited by 141 publications

(91 citation statements)

References 12 publications

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“…Analytical methods are focused mainly on protein or DNA molecules, which are extracted from the fish tissues. Owing to the protein denaturation caused by heating or canning (high temperature in combination with high pressure) processes, [5] DNA is a more suitable molecular marker for fish species authentication because it is more resistant to thermal treatment. Indeed, DNA is also degraded into small fragments during the thermal process but these are still detectable.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Piskatá

Pospisilova

Bořilová

2017

International Journal of Food Properties

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The most common techniques used to identify tuna species include methods based on the detection of specific DNA. The quality of species-specific DNA crucially affects the efficiency of amplification during the subsequent polymerase chain reaction (PCR). Detection of DNA in processed products can be adversely affected by DNA fragmentation during the processing steps and the use of ingredients that may inhibit the PCR reaction. In this study, several processing treatments applied to the muscle tissue of yellowfin tuna (Thunnus albacares) were evaluated. For DNA isolation three DNA extraction methods were compared. The concentration, purity, and amplificability of DNA were tested. The results revealed the variability among extraction procedures in terms of DNA quality and quantity in tuna muscle tissue processed under different processing technologies. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Piskatá

Pospisilova

Bořilová

2017

International Journal of Food Properties

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“…Traditional and official methods used in species identification, including fish, are based chiefly on the separation and characterization of specific proteins using electrophoretic techniques, such as isoelectric focusing: IEF (Rehbein 1990) and capillary electrophoresis: CE (Kvasnička 2005), high performance liquid chromatography: HPLC (Hubalkova et al 2007), or immunoassay systems, such as EnzymeLinked ImmunoSorbent Assay: ELISA ) (for a complete review see e.g., Mackie et al 1999;Civera 2003; Moretti et al 2003;Hubalkova et al 2007). Nowadays, different companies commercialize ELISA diagnostic kits for various applications, such as the authentication of species in milk and cheese ).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification methods of fish species: reassessment and possible applications

Teletchea

2009

Rev Fish Biol Fisheries

257

192

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Fish species identification is traditionally based on external morphological features. Yet, in many cases fishes and especially their diverse developmental stages are difficult to identify by morphological characters. DNA-based identification methods offer an analytically powerful addition or even an alternative. This work intends to provide an updated and extensive overview on the PCR-methods for fish species identification. Among the ten main methods developed, three PCR-RFLP, PCR-FINS and PCR-specific primers have been the most used. Two other emerging methods, namely real-time PCR and microarray technology, offer new potential for quantification of DNA and simultaneous detection of numerous species, respectively. Almost 500 species have been targeted in the past decade, among which the most studied belong to gadoids, scombroids, and salmonids. The mitochondrial cytochrome b gene was by far the most targeted DNA markers. The most common applications belonged to the forensic, taxonomic, and ecological fields. At last, some key problems, such as the degradation of DNA, the reliability of sequences, and the use of scientific names, likely to be encountered during the development of molecular identification methods are described. In conclusion, the tremendous advances in molecular biology in the past 10 years has rendered possible the study of DNA from virtually any substrates, offering new perspectives for the development of various applications, which will likely continue to increase in the future.

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“…Tahapan PCR terdiri dari predenaturasi 94˚C selama 5 menit, denaturasi 94 ukurannya lebih kecil, jumlah salinannya banyak, informasi urutan DNA tersedia lengkap untuk organisme akuatik, dan tidak ada rentang non-coding (Mackie et al 1999). DNA barcoding merupakan metode yang sering digunakan dalam forensik taksonomi karena efektif dalam mengidentifikasi dalam berbagai kondisi sampel uji dan tidak menghasilkan data yang ambigu (Dawnay et al 2007;Wong et al 2011).…”

Section: Amplifikasi Pcrunclassified

Molecular Characteristics of Cytochrome B for Mackerel Barcoding

et al. 2016

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AbstrakCytochrome b (cyt b) adalah salah satu gen pada DNA mitokondria yang sering digunakan sebagai marka molekuler untuk mengidentifikasi spesies melalui metode DNA barcoding. Penelitian ini bertujuan untuk menentukan karakteristik bioinformatika gen penyandi cyt b yang diisolasi dari ikan tenggiri (tenggiri papan dan tenggiri totol). Hasil amplifikasi PCR menunjukkan bahwa panjang DNA cyt b yang diisolasi dari ikan tenggiri papan 803 pasangan basa dengan komposisi purin 312 pasangan basa dan pirimidin 491 pasangan basa, sedangkan ikan tenggiri totol 791 pasangan basa dengan komposisi purin 316 pasangan basa dan pirimidin 475 pasangan basa. Analisis filogenetik menunjukkan bahwa kedua sampel termasuk kedalam kelompok Scomberomorus commerson dan Scomberomorus koreanus yang merupakan jenis ikan tenggiri. Titik isoelektrik cyt b ikan tenggiri papan 6,38 dan berat molekul 29826,23; ikan tenggiri totol 8,67 dan berat molekul 29372,77. Cyt b ikan tenggiri papan dan tenggiri totol memiliki kecenderungan hidrofobik. Dugaan pemodelan 3D cyt b pada kedua jenis ikan terdapat delapan bagian berbeda berdasarkan perbedaan warna yang ditampilkan. PENDAHULUANCytochrome b (cyt b) adalah salah satu gen yang ada pada DNA mitokondria dan sering digunakan dalam analisis DNA untuk mengidentifikasi spesies bahan baku pada produk perikanan (Sotelo et al. 2001). Identifikasi spesies menggunakan DNA mitokondria memiliki keuntungan karena

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Challenges in the identification of species of canned fish

Cited by 141 publications

References 12 publications

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Comparative study of DNA extraction methods from fresh and processed yellowfin tuna muscle tissue

Molecular identification methods of fish species: reassessment and possible applications

Molecular Characteristics of Cytochrome B for Mackerel Barcoding

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