“…This is not surprising given the influence of stream regulation on stream flow which causes numerous changes (Bunn & Arthington, 2002), affecting hydraulic patterns (velocity and depth) and variation on mayfly assemblage structures (Brooks et al, 2005;Klonowska-Olejnik & Skalski, 2014;Salmaso et al, 2018). With increasing stream flow variability and declining stream flow volumes in the Luvuvhu catchment due to increasing water abstraction and climate variability (Kleynhans, 1996;MacFadyen, Zambatis, Astrid, Van, & Cang, 2018;Ramulifho et al, 2019), this may decrease abundance or diversity of mayfly as well their ecosystem services (Boyero et al, 2011).…”