Challenging loop—mediated isothermal amplification (LAMP) technique for molecular detection of Toxoplasma gondii

Fallahi, Shirzad; Mazar, Zahra Arab; Ghasemian, Mehrdad; Haghighi, Ali

doi:10.1016/s1995-7645(14)60345-x

Cited by 46 publications

(30 citation statements)

References 42 publications

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“…In addition, this assay was able to detect HBV‐DNA as low as 10 2 IU/mL and below. In earlier studies, better performance, ie 10‐ to 1000‐fold higher sensitivity of the LAMP assay than PCR was observed for the detection of Arcobacter species and Toxoplasma gondii …”

Section: Discussionmentioning

confidence: 95%

Detection of Hepatitis B Virus DNA among Chronic and potential Occult HBV patients in resource‐limited settings by Loop‐Mediated Isothermal Amplification assay

Akram

Islam

Munshi

et al. 2018

Journal of Viral Hepatitis

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Transmission of Hepatitis B Virus (HBV) usually occurs due to the transfusion of blood or blood products from chronic HBV (CHB) or occult HBV-infected (OBI) patients. Besides serological tests, e.g. HBsAg and anti- HBc (total), detection of HBVDNA is necessary for the diagnosis of OBI patients. Different nucleic acid tests (NATs) including real- time-Polymerase Chain Reaction (qPCR) are used to detect HBV- DNA. The NATs are expensive and require technical expertise which are barriers to introduce them in resource-limited settings. This study was undertaken to evaluate the use of Loop-Mediated Isothermal Amplification (LAMP) assay as an alternative to qPCR for the detection of HBV-DNA in CHB and potential OBI patients in resource-limited settings. Following the published protocols with some modifications, a LAMP assay was developed for detection of HBV-DNA by either using a heat block followed by detection in an agarose gel or using a qPCR thermocycler. The LAMP assay was applied to supernatant prepared from heat-treated serum collected from CHB and potential OBI patients. HBV viral load in serum was measured by qPCR using a single-step HBV-DNA quantification kit. Among 200 samples tested, qPCR was capable to detect HBV-DNA in 25.5% of cases, whereas LAMP assay detected HBV-DNA in 43.5% cases. The qPCR was able to detect 11 (9.16%) potential OBI cases, whereas LAMP assay identified HBV-DNA in 43 (35.83%) cases. In addition to tests for HBsAg and/or anti-HBc (total), detection of HBV-DNA by LAMP assay may aid in preventing post-transfusion HBV infection in resource-limited settings.

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Section: Discussionmentioning

confidence: 95%

Detection of Hepatitis B Virus DNA among Chronic and potential Occult HBV patients in resource‐limited settings by Loop‐Mediated Isothermal Amplification assay

Akram

Islam

Munshi

et al. 2018

Journal of Viral Hepatitis

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“…Behavioral changes attributed to infection due to T. gondii may be due to the parasite itself, which causes infection with nerve damage. IFN-γ, the main responsible cytokine for immunological defense against T. gondii is essential in all infected tissues, including the central nervous system (13)(14)(15)(16)(17). The biosynthesis of dopamine and/or serotonin could be directly affected by aromatic amino acid hydroxylases in T. gondii genome.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii Infection in Patients with Alzheimer’s Disease

Rashno

Fallahi

Kheirandish

et al. 2016

Arch Clin Infect Dis

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Background: Toxoplasmosis is one of the most important diseases in humans and animals. Almost one-third of the human population around the world is infected with toxoplasmosis. The agent of this parasitic disease is a protozoan called Toxoplasma gondii (T. gondii) that causes encephalitis in people with suppressed immune systems and abortion, mental retardation and chorioretinitis in the fetus. Alzheimer's disease (AD) is the most important neurodegenerative disease. Objectives: Due to the high prevalence of toxoplasmosis in Iran and evidence on its impact on neurodegenerative diseases, this study was performed to evaluate the T. gondii infection in patients with AD. Patients and Methods:In this case-control study, after selection of alzheimer's patients (APs) referred to Imam Reza psychiatric hospital of Khorramabad, west of Iran, and healthy controls (each group consisted of 87 individuals), using the convenience sampling method and under the supervision of a neurologist, blood samples were taken during July 2014 and January 2015. The collected samples were transferred to the research laboratory of parasitology under cold chain storage and then, the serum samples were separated by centrifugation and were frozen at -20°C until use. The T. gondii IgM and IgG specific antibodies were assessed in serum samples using commercial Enzyme Linked Immunosorbent Assay (ELISA) kits. Results: The overall prevalence of T. gondii infection in patients with AD and the control group, using ELISA assay, was obtained as 66.6% (58/87) and 56.32% (49/87), respectively (P = 0.99). In this study, there was no significant association between T. gondii infection and AD. On the other hand, no statistically significant difference was observed between the two groups in terms of infection with T. gondii (P = 0.99). Conclusions: Higher prevalence of T. gondii in patients with AD compared to controls showed the possible impact of this parasite in AD, which may exacerbate symptoms, and this requires special attention of specialists and patient families.

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“…Hence, tests with high sensitivity and specificity are required (19)(20)(21)(22)(23) T. gondii. They demonstrated that electrochemiluminescence is a useful method to detect T. gondii infection with high sensitivity and specificity of 97.1% and 100.0%, respectively (24).…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma Gondii Infection and Related Risk Factors Among Blood Donors in Northwest Iran

Bahhaj

Ahmadpour

Mahami-Oskouei

et al. 2017

Arch Clin Infect Dis

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Background: Toxoplasmosis, a worldwide parasitic infection, is considered as a transfusion-transmitted disease. Objectives: Due to ignorance about Toxoplasma infection in blood donors monitoring and its important role in provision of safe blood and blood products and also poor information about the seroprevalence of T. gondii infection in blood donors, this study was conducted to assess anti-Toxoplasma gondii antibodies and associated risk factors among blood donors in northwest of Iran.

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Challenging loop—mediated isothermal amplification (LAMP) technique for molecular detection of Toxoplasma gondii

Cited by 46 publications

References 42 publications

Detection of Hepatitis B Virus DNA among Chronic and potential Occult HBV patients in resource‐limited settings by Loop‐Mediated Isothermal Amplification assay

Detection of Hepatitis B Virus DNA among Chronic and potential Occult HBV patients in resource‐limited settings by Loop‐Mediated Isothermal Amplification assay

Seroprevalence of Toxoplasma gondii Infection in Patients with Alzheimer’s Disease

Toxoplasma Gondii Infection and Related Risk Factors Among Blood Donors in Northwest Iran

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