Change in cell surface properties of Lactobacillus casei under heat shock treatment

Abstract

We undertake this study in the aim to give new insight about the change in cellular physiological state under heat shock treatment and probiotic strain screening procedure. Different cell properties have been studied like adhesive ability to biotic and abiotic surfaces, the cell surface hydrophobicity and the fatty acids profiles. Compared to the normal cells, the heated cells increased their adhesive ability to biotic surface. However, the adhesion to abiotic surface was decreased. The cell surface hydrophobicity of the heated strains showed a significant decrease (P < 0.05). Our data revealed that high temperature change the fatty acids profiles of the treated cells, especially the proportions of unsaturated and saturated fatty acid. In fact, the ratio of saturated to unsaturated fatty acids of the heated Lactobacillus casei cells was significantly higher than that of the control cells (P < 0.05). The present finding could firstly add new insight about the response of probiotic to stressful conditions, such us the important role of cell membrane, considered as the first main structure to be damaged by physicochemical stress, in stress resistance because of their composition that can change in adaptation to harsh conditions. Secondly, there is no relationship between changes in membrane composition and fluidity induced by heat shock treatment and adhesion to biotic and abiotic surface.

In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains

“…Generally, adhesion is a complex process involving non-specific (hydrophobic interactions between cell surfaces) and specific ligand-receptor interactions [6]. Different structures and specific cell wall components of bacteria, such as fimbriae or pili, adhesins, mucus-binding proteins, fibronectin-binding proteins, or surface layer proteins, provide them an advantage for epithelial colonization; further, lipoteichoic acid or exopolysaccharides produced by bacteria can contribute to adhesion to host epithelial cells [12,13,15,16]. Interactions among all these properties important for adhesion provide the benefit of intestine colonization to probiotic strains.…”

How to routinely assess transition, adhesion and survival of probiotics into the gut: a case study on propionibacteria

“…Some authors reported that two mechanisms are probably involved in the adhesion of probiotics to gut mucosa: specific adhesion to host intestinal epithelial surfaces (IESM) and unspecific adhesion to hydrophobic surfaces (de Wouters et al, 2015). The first line of contact is the mucus which acts as a protective layer due to its hydrophobic nature, thus the hydrophobicity of bacterial surface could play a significant role favouring the contact with host and promoting the second stage of adhesion by specific cell wall components (Haddaji et al, 2015). Hydrophobicity of cell envelope has been proposed as a simple screening index to test the ability of bacteria or yeasts to adhere to mucosa (Dhewa et al, 2009;Bautista-Gallego et al, 2013;Perricone et al, 2014a;Tokatli et al, 2015;Corbo et al, 2017;Speranza et al, 2017).…”

Characterization of a Sea Buckthorn Extract and Its Effect on Free and Encapsulated Lactobacillus casei

“…One of the main factors that vary from one Lactobacillus spp. to another is the potential of the strain to resist at high temperatures [ 43 , 44 , 45 , 46 ]. Gunenc et al [ 16 ] reported an increase of 6% in the case where whole sea buckthorn was utilized with a Lactobacillus strain during storage over 14 days in comparison with the sample where no sea buckthorn was added.…”