In Vitro Evaluation of Adhesion Capacity, Hydrophobicity, and Auto-Aggregation of Newly Isolated Potential Probiotic Strains

Abstract

We isolated several strains of human and animal origin, focusing on the genera Lactobacillus and Bifidobacterium. Samples of cow colostrum, feces and saliva of calves and piglets, and infant feces were acquired, bacterial strains were isolated and identified, and twenty strains of lactobacilli and bifidobacteria were shortlisted for further in vitro analysis of adhesion capacity to human cells, surface hydrophobicity, and auto-aggregation. Adhesion capacity was evaluated using a mixture of Caco-2 and HT-29-MTX cells and hydrophobicity was measured using the microbial adhesion to the hydrocarbon method. Lactobacillus reuteri was the most frequently isolated species, followed by L. casei subsp. paracasei and L. gasseri; all strains were isolated from infant feces. Bifidobacteria were represented by the species B. longum (infant feces), and B. thermophilum and B. pseudolongum (calves, piglets). All twenty strains showed good adhesion capacities to the mixed cell culture (17.7–37.2%), particularly, L. reuteri isolates K7 and K14 (37.2% and 35.5%, respectively). In hydrophobicity and auto-aggregation assays, strain-specific differences irrespective of the origin or taxonomic group were observed. Hydrophobicity values varied considerably (from 6.1% to 87.4%), whereas auto-aggregation ability ranged from 21.7% to 69.7%. No relation was observed between hydrophobicity and adhesion capacity; instead, auto-aggregation was apparently related with adhesion.

Screening of GABA-Producing Lactic Acid Bacteria from Thai Fermented Foods and Probiotic Potential of Levilactobacillus brevis F064A for GABA-Fermented Mulberry Juice Production

“…L. brevis F064A showed a lower hydrophobicity level. The hydrophobicity property indicates the adhesion capacity on epithelial cells of probiotic bacteria but it does not confirm that a high level of hydrophobicity can show a strong adhesion to mucosa or epithelial cells [ 50 , 51 ]. Additionally, the hydrophobicity ability had a high variation even in the same strain of bacteria such as 7 strains of L. fermentum present a variation of hydrophobicity in a range from 0.30–68.81% [ 51 ].…”

Isolation of Novel Probiotic Lactobacillus and Enterococcus Strains From Human Salivary and Fecal Sources

“…The auto-aggregation test evaluates the ability of isolates to adhere to the intestine, exerting antipathogenic effects (Krausova et al, 2019). This test was performed using the method by Xu et al (2009).…”

Characterization, in-Vitro Evaluation of Probiotic Potential and Antagonistic Activity of Selected Lactic Acid Bacteria Strains Isolated From Natural Origin Against Some Human Pathogens

“…However, this value was more than 3 times greater than what was reported by Xu et al 40 . The wide variation of auto-aggregation ability among our tested isolates could be explained according to Krausova et al who illustrated that autoaggregation assays done among probiotic bacteria revealed strain-specific variations irrespective of their taxonomic group or origin 41 . LAB isolates under study showed inhibitory activity against both Gram-negative and Gram-positive bacteria.…”