Change in colony morphology and kinetics of tylosin production after UV and gamma irradiation mutagenesis of Streptomyces fradiae NRRL-2702

Abstract

Tylosin is a macrolide antibiotic used as veterinary drug and growth promoter. Attempts were made for hyper production of tylosin by a strain of Streptomyces fradiae NRRL-2702 through irradiation mutagenesis. Ultraviolet (UV) irradiation of wild-type strain caused development of six morphologically altered colony types on agar plates. After screening using Bacillus subtilis bioassay only morphological mutants indicated the production of tylosin. An increase of 2.7+/-0.22-fold in tylosin production (1500mg/l) in case of mutant UV-2 in complex medium was achieved as compared to wild-type strain (550mg/l). Gamma irradiation of mutant UV-2 using (60)Co gave one morphologically altered colony type gamma-1, which gave 2500mg/l tylosin yield in complex medium. Chemically defined media promoted tylosin production upto 3800mg/l. Maximum value of q(p) (3.34mg/gh) was observed by mutant gamma-1 as compared to wild strain (0.81mg/gh). Moreover, UV irradiation associated changes were unstable with loss of tylosin activity whereas mutant gamma-1 displayed high stability on subsequent culturing.

Improvement of paromomycin production by Streptomyces rimosus subsp paromomycinus NRRL 2455 using gamma irradiation mutagenesis

“…Additionally in our findings, Successive culturing of the mutant 5M revealed great genetic stability. Similar results were reported for Streptomyces fradiae NRRL-2702 mutant gamma-1, however, UV irradiation associated changes were unstable with loss of tylosin activity [24]. These results make gamma radiation an effective way for mutation compared to other mutagens.…”

Galur Isogenik Xanthomonas oryzae pv. oryzae Hasil Penyinaran Ultraviolet dan Potensinya Sebagai Penginduksi Resistensi Padi terhadap Penyakit Hawar Daun Bakteri

“…Mutan Streptomyces fradiae NRRL-2702 mengalami perubahan morfologi dari tipe liarnya setelah diberi pemaparan UV (Khaliq et al 2007 Gambar 1 Morfologi Xanthomonas oryzae pv. oryzae M101 berubah dibandingkan dengan tipe liarnya pada medium agar-agar sukrosa kentang (5 hsi), perubahan terlihat pada bentuk, warna, pinggiran, dan lendir pada permukaan koloni.…”

Enhancement of pectinase production by ultraviolet irradiation and diethyl sulfate mutagenesis of a Fusarium oxysporum isolate

“…The treatment of spores and vegetative mycelium with different mutagens to screen for improved mutants among the surviving progeny has been recognized as an effective means of obtaining strains with improved potency (Khaliq et al, 2009, Romdhane et al, 2013. Strain mutation induction and screening techniques can improve microbes' pectinase yield by using different mutagens such as nitrous acid, diethyl sulfate (DES), and ethyl methyl sulfonate.…”