Change in the Membranous Lipid Composition Accelerates Lipid Peroxidation in Young Rat Hearts Subjected to 2 Weeks of Hypoxia Followed by Hyperoxia

Oka, Tatsujiro; Itoi, Toshiyuki; Terada, Naoto; Nakanishi, Hiroki; Taguchi, Ryo; Hamaoka, Kenji

doi:10.1253/circj.72.1359

Cited by 18 publications

(15 citation statements)

References 30 publications

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“…32 O-acetylcarnitine and carnitine are reported in oxidative metabolism in mitochondrial 30 and hypoxemic stress. 33,34 Adenine is required for the production of adenosine, 30 an endogenous purine nucleoside important in cellular energy metabolism. In response to cellular damage, levels of adenosine typically rise.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic profiling of asthma: Diagnostic utility of urine nuclear magnetic resonance spectroscopy

Saude

Skappak

Regush

et al. 2011

Journal of Allergy and Clinical Immunology

152

159

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Section: Discussionmentioning

confidence: 99%

Metabolomic profiling of asthma: Diagnostic utility of urine nuclear magnetic resonance spectroscopy

Saude

Skappak

Regush

et al. 2011

Journal of Allergy and Clinical Immunology

152

159

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“…In addition, newborns have an increased risk of cellular injury related to carnitine deficiency because of the immaturity of the carnitine biosynthetic pathways [23], [24]. L-carnitine can suppress lipid peroxydation after ischemia reperfusion [25] and can suppress hydroxyl radical production in the Fenton reaction. Together with propionyl-L-carnitine (C3), L-carnitine possesses potent antioxidant activity [26].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Analyses of Plasma Reveals New Insights into Asphyxia and Resuscitation in Pigs

et al. 2010

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BackgroundCurrently, a limited range of biochemical tests for hypoxia are in clinical use. Early diagnostic and functional biomarkers that mirror cellular metabolism and recovery during resuscitation are lacking. We hypothesized that the quantification of metabolites after hypoxia and resuscitation would enable the detection of markers of hypoxia as well as markers enabling the monitoring and evaluation of resuscitation strategies.Methods and FindingsHypoxemia of different durations was induced in newborn piglets before randomization for resuscitation with 21% or 100% oxygen for 15 min or prolonged hyperoxia. Metabolites were measured in plasma taken before and after hypoxia as well as after resuscitation. Lactate, pH and base deficit did not correlate with the duration of hypoxia. In contrast to these, we detected the ratios of alanine to branched chained amino acids (Ala/BCAA; R2.adj = 0.58, q-value<0.001) and of glycine to BCAA (Gly/BCAA; R2.adj = 0.45, q-value<0.005), which were highly correlated with the duration of hypoxia. Combinations of metabolites and ratios increased the correlation to R2adjust = 0.92. Reoxygenation with 100% oxygen delayed cellular metabolic recovery. Reoxygenation with different concentrations of oxygen reduced lactate levels to a similar extent. In contrast, metabolites of the Krebs cycle (which is directly linked to mitochondrial function) including alpha keto-glutarate, succinate and fumarate were significantly reduced at different rates depending on the resuscitation, showing a delay in recovery in the 100% reoxygenation groups. Additional metabolites showing different responses to reoxygenation include oxysterols and acylcarnitines (n = 8–11, q<0.001).ConclusionsThis study provides a novel strategy and set of biomarkers. It provides biochemical in vivo data that resuscitation with 100% oxygen delays cellular recovery. In addition, the oxysterol increase raises concerns about the safety of 100% O2 resuscitation. Our biomarkers can be used in a broad clinical setting for evaluation or the prediction of damage in conditions associated with low tissue oxygenation in both infancy and adulthood. These findings have to be validated in human trials.

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“…L-carnitine (dietary supplement): 1 ml containing 250 mg carnitine was purchased from the Arab Company for Pharmaceuticals Medicinal Plants (MEPACO, Egypt (Enshas El Raml-Sharkeiya). Oral administration of a dose of 250 mg/kg daily was done according to the method described by Oka et al [ 23 ]. Handling of the animal was the same for all groups and did not affect weight gain.…”

Section: Methodsmentioning

confidence: 99%

Effect of Carnitine and herbal mixture extract on obesity induced by high fat diet in rats

Amin

Nagy

2009

Diabetol Metab Syndr

145

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Change in the Membranous Lipid Composition Accelerates Lipid Peroxidation in Young Rat Hearts Subjected to 2 Weeks of Hypoxia Followed by Hyperoxia

Cited by 18 publications

References 30 publications

Metabolomic profiling of asthma: Diagnostic utility of urine nuclear magnetic resonance spectroscopy

Metabolomic profiling of asthma: Diagnostic utility of urine nuclear magnetic resonance spectroscopy

Metabolomic Analyses of Plasma Reveals New Insights into Asphyxia and Resuscitation in Pigs

Effect of Carnitine and herbal mixture extract on obesity induced by high fat diet in rats

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