Change of Biological Parameters of Poultry Semen  at Cryopreservation

Иолчиев, Б. С.; Багиров, В. А.; Zhilinskiy, M. A.; Волкова, Н. А.; Zinovieva, N. A.

doi:10.15389/agrobiology.2018.6.1230eng

Cited by 2 publications

(4 citation statements)

References 22 publications

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“…Almasi et al (2002) and Varga et al (2003) who analyzed the fresh semen of Hungarian ganders also pointed a low semen quality of local goose breeds kept as gene pool flock. The percentage of live sperm was lower compared to our results obtained in the northern and southern varieties, as also observed by Iolchiev et al (2018) in Anser anser L. goose semen and Tai et al (2001) in Chinese brown goose, that also has been observed in Biłgorajska (Opałka et al, 2008) and Kuban goose (Chełmońska et al, 1984).…”

Section: Discussionsupporting

confidence: 76%

See 1 more Smart Citation

Freezability of Semen Collected from Ganders of 12 Breeds Covered by the Polish Genetic Resources Conservation Program

Łukaszewicz,

Kowalczyk,

Jerysz

et al. 2024

Annals of Animal Science

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A rapid decline in wild animal biodiversity, as well as in the number of local livestock breeds of lower economical values, makes species and breeds protection and creation of genetic reserves a necessity and a challenge of the 21st century. For birds, semen freezing is still the best method of gene preservation ex situ in vitro. The aim of the presented study was to assess the susceptibility to freezing process and to establish the semen gene bank of twelve goose breeds covered by the Polish genetic resources conservation program. Sexually mature ganders were randomly selected from four northern local breeds: Kartuska, Pomorska, Rypinska, Suwalska, four southern local breeds: Garbonosa, Kielecka, Lubelska, Podkarpacka and four foreign goose breeds: Kuban, Landes, Roman and Slovakia. Each breed was represented by 12 males, twelve repetitions were carried out. Semen samples were frozen in a computer-controlled cryogenic chamber using a method developed for White Koluda goose. The basic evaluation criterion was sperm morphology in fresh and freeze-thawed semen assessed in nigrosine-eosin stained histological smears. The percentage of live in total and live normal sperm in the fresh semen differed significantly (P<0.05) between breeds. Semen of Kartuska and Suwalska ganders (northern breeds), contained the highest percent of live sperm - 94.4 and 94.1 respectively, including 52.5% and 54.2% of live normal sperm, while the lowest number of these forms were observed for Slovakia goose – 86.4% and 29.2%, respectively. The freezing process resulted in a significant (P<0.05) reduction in the total number of live and live normal sperm and an increase in dead sperm in all groups. Cryopreservation process was the most effective in case of Suwalska goose semen (28.4% of live normal cells in thawed semen) and the less profitable for Landes (7.4% of live normal cells). The analyzes showed significant differences between breeds in sperm morphology in fresh and thawed semen. Moreover, the high proportion of live spermatozoa in the fresh semen did not guarantee a high proportion of spermatozoa after thawing, indicating the differences in semen susceptibility to cryopreservation process depending on goose breed.

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Section: Discussionsupporting

confidence: 76%

“…Our results were also confirmed by reports of the other authors. Iolchiev et al (2018) reported a decrease in the number of live sperm in semen of Anser anser goose and Tai et al (2001) in the Chinese brown goose, while Dubos et al (2006) andVaradi et al (2019) in semen of Landes ganders.…”

Section: Discussionmentioning

confidence: 96%

Freezability of Semen Collected from Ganders of 12 Breeds Covered by the Polish Genetic Resources Conservation Program

Łukaszewicz,

Kowalczyk,

Jerysz

et al. 2024

Annals of Animal Science

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“…In Russia, studies in this direction are being conducted at the L.K. Ernst Federal Science Center for Animal Husbandry and its branch, the Russian Research Institute of Farm Animal Genetics and Breeding (Iolchiev et al, 2018;Mavrodina et al, 2018a, b;Stanishevskaya, Pleshanov, 2018a-c).…”

Section: Semen Preservationmentioning

confidence: 99%

“…The causes of fertility loss include the susceptibility to cold shock, cooling rate, diluent composition, and osmotic stress. There are also factors that affect the functional state of frozen/ thawed spermatozoa: membrane stability, oxidative damage, membrane receptor integrity, and nucleus structure (Watson, 2000;Iolchiev et al, 2018). In the course of cryopreservation and thawing, sperm can experience both irreversible damage, expressed as the absence of motility and various morphological disorders, and reversible, associated mainly with a temporary injury of the structure and membrane permeability disturbance.…”

Section: Problems Of Cryopreservationmentioning

confidence: 99%

The current state of the problem of in vitrogene pool preservation in poultry

2020

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This review presents the current progress in and approaches to in vitroconservation of reproductive cells of animals, including birds, such as cryopreservation and freeze-drying, as well as epigenetic conditions for re storing viable spermatozoa and female gametes after conservation. Cryopreservation is an effective way to preserve reproductive cells of various species of animals and birds. In vitrogene pool conservation is aimed primarily to the restoration of extinct breeds and populations and to the support of genetic diversity in populations prone to genetic drift. It is the combination of ex situ in vivoand ex situ in vitromethods that can form the basic principles of the strategy of animal genetic diversity preservation. Also, use of cryopreserved semen allows faster breeding in industrial poultry farming. Despite numerous advances in semen cryobiology, new methods that can more efficiently restore semen fertility after cryopreservation are being sought. The mechanisms underlying the effect of cryopreservation on the semen parameters of cocks are insufficiently understood. The review reflects the results of recent research in the field of cryopreservation of female and male germ cells, embryonic cells, the search for new ways in the field of genetic diversity in vitro (the development of new cryoprotective media and new conservation technologies: freeze-drying). Molecular aspects of cryopreservation and the mechanisms of cryopreservation influence on the epigenetic state of cells are highlighted. Data on the results of studies in the field of male reproductive cell lyophilization are presented. The freeze-drying of reproductive cells, as a technology for cheaper access to the genetic material of wild and domestic animals, compared to cryopreservation, attracts the attention of scientists in Japan, Israel, Egypt, Spain, and France. There is growing interest in the use of lyophilized semen in genetic engineering technologies. Methods of freeze-drying are developed taking into account the species of birds. Organizational and legal ways of solving the problems of in vitroconservation of genetic resources of farm animals, including birds, are proposed.

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Change of Biological Parameters of Poultry Semen at Cryopreservation

Cited by 2 publications

References 22 publications

Freezability of Semen Collected from Ganders of 12 Breeds Covered by the Polish Genetic Resources Conservation Program

Freezability of Semen Collected from Ganders of 12 Breeds Covered by the Polish Genetic Resources Conservation Program

The current state of the problem of in vitrogene pool preservation in poultry

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