Changes in bacterial activity and community composition caused by exposure to a simulated oil spill in microcosm and mesocosm experiments

Lekunberri, Itziar; Calvo‐Díaz, Alejandra; Teira, Eva; Morán, Xosé Anxelu G.; Peters, Francesc; Nieto‐Cid, Mar; Espinoza-González, Oscar; Teixeira, I. G.; Gasol, Josep M.

doi:10.3354/ame01380

Cited by 21 publications

(11 citation statements)

References 67 publications

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“…Cell-specific BP rates in the Ambient CO 2 treatment (5.4 and 13.1 fg C cell −1 d −1 for Cytophaga and Roseobacter, respectively) are within the range of cell-specific BP rates measured in coastal waters where members of Rhodobacteraceae and Flavo bacteriaceae are particularly abundant (Reinthaler & Herndl 2005, Lamy et al 2009, Lekunberri et al 2010.…”

Section: Discussionmentioning

confidence: 77%

“…Based on microscope estimates, we found that the mean biovolume of Roseobacter cells was twice that of Cytophaga cells. The higher FPOM-T in Roseobacter than in Cytophaga cultures also suggests higher bacterial biomass in the Roseo bacter cultures.Cell-specific BP rates in the Ambient CO 2 treatment (5.4 and 13.1 fg C cell −1 d −1 for Cytophaga and Roseobacter, respectively) are within the range of cell-specific BP rates measured in coastal waters where members of Rhodobacteraceae and Flavo bacteriaceae are particularly abundant (Reinthaler & Herndl 2005, Lamy et al 2009, Lekunberri et al 2010.Heterotrophs can assimilate CO 2 in various carboxylation reactions as part of central and peripheral pathways (Dijkhuizen & Harder 1985). We found that CO 2 fixation was independent of light in both strains, which points out to a purely heterotrophic assimilation process.…”

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confidence: 85%

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Response of two marine bacterial isolates to high CO<sub>2 concentration

Teira¹,

Fernández²,

Álvarez‐Salgado³

et al. 2012

Mar. Ecol. Prog. Ser.

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Experimental results related to the effects of ocean acidification on planktonic marine microbes are still rather inconsistent and occasionally contradictory. Moreover, laboratory or field experiments that address the effects of changes in CO 2 concentrations on heterotrophic microbes are very scarce, despite the major role of these organisms in the marine carbon cycle. We tested the direct effect of an elevated CO 2 concentration (1000 ppmv) on the biomass and metabolic rates (leucine incorporation, CO 2 fixation and respiration) of 2 isolates belonging to 2 relevant marine bacterial families, Rhodobacteraceae (strain MED165) and Flavobacteriaceae (strain MED217). Our results demonstrate that, contrary to some expectations, high p CO 2 did not negatively affect bacterial growth but increased growth efficiency in the case of MED217. The elevated partial pressure of CO 2 (pCO 2 ) caused, in both cases, higher rates of CO 2 fixation in the dissolved fraction and, in the case of MED217, lower respiration rates. Both responses would tend to increase the pH of seawater acting as a negative feedback between elevated atmospheric CO 2 concentrations and ocean acidification. KEY WORDS: Bacterial metabolism · Flavobacteriaceae · Ocean acidification · RhodobacteraceaeResale or republication not permitted without written consent of the publisher Mar Ecol Prog Ser 453: 27-36, 2012 on marine bacterial isolates (Takeuchi et al. 1997, Labare et al. 2010. The latter studies found a decrease in the production and growth rates at pH < 7 -values far from the usual pH observed in ocean waters under present or future scenarios of elevated p CO 2 .Most microorganisms, particularly heterotrophic bacteria, are able to assimilate CO 2 as part of their metabolism through anaplerotic reactions (Roslev et al. 2004). Although light-independent or dark CO 2 assimilation has been usually assumed to be insignificant in oxygenated marine waters, a recent work by Alonso-Sáez et al. (2010) suggests that the global relevance of this process could have been underestimated. Those results show for the first time that high ambient CO 2 concentrations could stimulate CO 2 fixation rates by increasing the CO 2 flux into the cells.A comprehensive understanding of the effect of elevated CO 2 concentration on carbon cycling in the ocean requires the analysis of both production and respiration rates to provide a total carbon budget. However, to the best of our knowledge none of the published studies have simultaneously addressed the effect of CO 2 on BP and respiration, which are essential variables for bacterial growth efficiency (BGE) calculations. Allgaier et al. (2008) did find changes in bacterial taxonomic composition in response to high CO 2 concentrations, which suggest that the effects of elevated p CO 2 are likely to vary among species. Therefore, the aim of the present study was to test the direct effect of elevated CO 2 concentrations (1000 ppmv) on the biomass and metabolic rates (leucine incorporation, CO 2 fixation and respiration...

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Section: Discussionmentioning

confidence: 77%

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confidence: 85%

Response of two marine bacterial isolates to high CO<sub>2 concentration

Teira¹,

Fernández²,

Álvarez‐Salgado³

et al. 2012

Mar. Ecol. Prog. Ser.

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“…However, our ability to understand and predict the dynamics of in situ bacterial communities responding to environmental stimuli such as the presence of oil contamination remains in its infancy (27,60). The majority of previous work on hydrocarbon-degrading bacterial communities in the marine environment has been conducted under enrichment conditions in laboratory microcosms (12,13,45,52,57,69,75). Of the fewer studies that have been conducted in the field, most have focused on biostimulation, for instance the application of nutrients to enhance oil bioremediation (46,49,64,68).…”

Section: Discussionmentioning

confidence: 99%

Hydrocarbon-Degrading Bacteria and the Bacterial Community Response in Gulf of Mexico Beach Sands Impacted by the Deepwater Horizon Oil Spill

Kostka

Prakash

Overholt

et al. 2011

Appl Environ Microbiol

771

508

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A significant portion of oil from the recent Deepwater Horizon (DH) oil spill in the Gulf of Mexico was transported to the shoreline, where it may have severe ecological and economic consequences. The objectives of this study were (i) to identify and characterize predominant oil-degrading taxa that may be used as model hydrocarbon degraders or as microbial indicators of contamination and (ii) to characterize the in situ response of indigenous bacterial communities to oil contamination in beach ecosystems. This study was conducted at municipal Pensacola Beach, FL, where chemical analysis revealed weathered oil petroleum hydrocarbon (C 8 to C 40 ) concentrations ranging from 3.1 to 4,500 mg kg ؊1 in beach sands. A total of 24 bacterial strains from 14 genera were isolated from oiled beach sands and confirmed as oil-degrading microorganisms. Isolated bacterial strains were primarily Gammaproteobacteria, including representatives of genera with known oil degraders (Alcanivorax, Marinobacter, Pseudomonas, and Acinetobacter). Sequence libraries generated from oiled sands revealed phylotypes that showed high sequence identity (up to 99%) to rRNA gene sequences from the oil-degrading bacterial isolates. The abundance of bacterial SSU rRNA gene sequences was ϳ10-fold higher in oiled (0.44 ؋ 10 7 to 10.2 ؋ 10 7 copies g ؊1 ) versus clean (0.024 ؋ 10 7 to 1.4 ؋ 10 7 copies g ؊1 ) sand. Community analysis revealed a distinct response to oil contamination, and SSU rRNA gene abundance derived from the genus Alcanivorax showed the largest increase in relative abundance in contaminated samples. We conclude that oil contamination from the DH spill had a profound impact on the abundance and community composition of indigenous bacteria in Gulf beach sands, and our evidence points to members of the Gammaproteobacteria (Alcanivorax, Marinobacter) and Alphaproteobacteria (Rhodobacteraceae) as key players in oil degradation there.

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“…Most of the studies investigating the effect of anthropogenic pollution on bacterial communities were performed under artificial conditions, with high concentration of the targeted pollutants. This is particularly the case for hydrocarbons in pelagic environments, where most of the studies were done in microcosms under controlled conditions with concentrations close or even higher than oil spills (Cappello et al 2007;Gertler et al 2009;Lekunberri et al 2010). Even if such experiments present the advantage of raising clear tendencies, field investigations remain critical for understanding in situ processes, but such investigations remain scarce.…”

Section: Introductionmentioning

confidence: 95%

Influence of PAHs among other coastal environmental variables on total and PAH-degrading bacterial communities

Sauret

Tedetti

Guigue

et al. 2015

Environ Sci Pollut Res

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We evaluated the relative impact of anthropogenic polycyclic aromatic hydrocarbons (PAHs) among biogeochemical variables on total, metabolically active, and PAH bacterial communities in summer and winter in surface microlayer (SML) and subsurface seawaters (SSW) across short transects along the NW Mediterranean coast from three harbors, one wastewater effluent, and one nearshore observatory reference site. At both seasons, significant correlations were found between dissolved total PAH concentrations and PAH-degrading bacteria that formed a gradient from the shore to nearshore waters. Accumulation of PAH degraders was particularly high in the SML, where PAHs accumulated. Harbors and wastewater outfalls influenced drastically and in a different way the total and active bacterial community structure, but they only impacted the communities from the nearshore zone (<2 km from the shore). By using direct multivariate statistical analysis, we confirmed the significant effect of PAH concentrations on the spatial and temporal dynamic of total and active communities in this area, but this effect was putted in perspective by the importance of other biogeochemical variables.

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Changes in bacterial activity and community composition caused by exposure to a simulated oil spill in microcosm and mesocosm experiments

Cited by 21 publications

References 67 publications

Response of two marine bacterial isolates to high CO<sub>2 concentration

Response of two marine bacterial isolates to high CO<sub>2 concentration

Hydrocarbon-Degrading Bacteria and the Bacterial Community Response in Gulf of Mexico Beach Sands Impacted by the Deepwater Horizon Oil Spill

Influence of PAHs among other coastal environmental variables on total and PAH-degrading bacterial communities

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