Changes in biofilm formation in the nonflagellated flhB1 mutant of Azospirillum brasilense Sp245

Шелудько, А. В.; Filip’echeva, Yu. A.; Shumilova, E. M.; Khlebtsov, Boris N.; Буров, А. М.; Petrova, L. P.; Кацы, Е. И.

doi:10.1134/s0026261715010129

Cited by 20 publications

(6 citation statements)

References 18 publications

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“…The genes nirK ( nirK1 and nirK2 ) were found to encode NO through nitrite reductase, which modulates biofilm formation and subsequent root colonization . Retention of a polar flagellum encoded by flhB gene in A. brasilense Sp245 enhanced the biofilm formation and stability .…”

Section: Agriculturally Important Microbial Biofilmsmentioning

confidence: 99%

“…The genes nirK (nirK1 and nirK2) were found to encode NO through nitrite reductase, which modulates biofilm formation and subsequent root colonization [278,279]. Retention of a polar flagellum encoded by flhB gene in A. brasilense Sp245 enhanced the biofilm formation and stability [280]. Whole genome transcriptome of A. chroococcum, revealed different genes involved in Alginate biosynthesis and regulation (algD,8,44,K,E,G,X,L,I,F,A,algC), capsule lipopolysaccharide production (lptCAD, lptD), and Cellulose and Levan synthesis (yhjQ, bcsA/B, bcsC, bcs D, and bcsZ) [281].…”

Section: Genes Involved In Biofilm Formationmentioning

confidence: 99%

“…Enhance the biofilm stability [280] Azotobacter chroococcum algD,8,44,K,E,G,X,L,I,F,A,algC, Alginate biosynthesis and regulation [281] algF, algR Capsule lipopolysaccharides (LPS) lptCAD, lptD yhjQ, bcsA/B, bcsC, bcs D, and bcsZ…”

Section: Genes Involved In Biofilm Formationmentioning

confidence: 99%

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Agriculturally important microbial biofilms: Present status and future prospects

2017

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Microbial biofilms are a fascinating subject, due to their significant roles in the environment, industry, and health. Advances in biochemical and molecular techniques have helped in enhancing our understanding of biofilm structure and development. In the past, research on biofilms primarily focussed on health and industrial sectors; however, lately, biofilms in agriculture are gaining attention due to their immense potential in crop production, protection, and improvement. Biofilms play an important role in colonization of surfaces - soil, roots, or shoots of plants and enable proliferation in the desired niche, besides enhancing soil fertility. Although reports are available on microbial biofilms in general; scanty information is published on biofilm formation by agriculturally important microorganisms (bacteria, fungi, bacterial-fungal) and their interactions in the ecosystem. Better understanding of agriculturally important bacterial-fungal communities and their interactions can have several implications on climate change, soil quality, plant nutrition, plant protection, bioremediation, etc. Understanding the factors and genes involved in biofilm formation will help to develop more effective strategies for sustainable and environment-friendly agriculture. The present review brings together fundamental aspects of biofilms, in relation to their formation, regulatory mechanisms, genes involved, and their application in different fields, with special emphasis on agriculturally important microbial biofilms.

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Section: Agriculturally Important Microbial Biofilmsmentioning

confidence: 99%

Section: Genes Involved In Biofilm Formationmentioning

confidence: 99%

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Agriculturally important microbial biofilms: Present status and future prospects

2017

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“…It should be emphasized that studies specially devoted to biofilms formed by Azospirillum spp. have been relatively limited (see, e.g., reports [ 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ] (and some earlier references cited therein) on various biochemical and physiological properties of biofilms formed by these bacteria, including a report on dual-species biofilms [ 51 ]), so this important field for azospirilla is yet to be investigated in more detail.…”

Section: Introductionmentioning

confidence: 99%

Fourier Transform Infrared (FTIR) Spectroscopic Study of Biofilms Formed by the Rhizobacterium Azospirillum baldaniorum Sp245: Aspects of Methodology and Matrix Composition

et al. 2023

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Biofilms represent the main mode of existence of bacteria and play very significant roles in many industrial, medical and agricultural fields. Analysis of biofilms is a challenging task owing to their sophisticated composition, heterogeneity and variability. In this study, biofilms formed by the rhizobacterium Azospirillum baldaniorum (strain Sp245), isolated biofilm matrix and its macrocomponents have for the first time been studied in detail, using Fourier transform infrared (FTIR) spectroscopy, with a special emphasis on the methodology. The accompanying novel data of comparative chemical analyses of the biofilm matrix, its fractions and lipopolysaccharide isolated from the outer membrane of the cells of this strain, as well as their electrophoretic analyses (SDS-PAGE) have been found to be in good agreement with the FTIR spectroscopic results.

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“…A . brasilense cells are also capable of forming biofilms on both abiotic surface [15, 16] and in association with host plants; when inoculated onto sterile wheat roots, azospirilla colonize the root surfaces extensively, as well as the sites of lateral root emergence [15].…”

Section: Introductionmentioning

confidence: 99%

TyrR is involved in the transcriptional regulation of biofilm formation and D-alanine catabolism in Azospirillum brasilense Sp7.

et al. 2019

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Azospirillum brasilense is one of the most studied species of diverse agronomic plants worldwide. The benefits conferred to plants inoculated with Azospirillum have been primarily attributed to its capacity to fix atmospheric nitrogen and synthesize phytohormones, especially indole-3-acetic acid (IAA). The principal pathway for IAA synthesis involves the intermediate metabolite indole pyruvic acid. Successful colonization of plants by Azospirillum species is fundamental to the ability of these bacteria to promote the beneficial effects observed in plants. Biofilm formation is an essential step in this process and involves interactions with the host plant. In this study, the tyrR gene was cloned, and the translated product was observed to exhibit homology to TyrR protein, a NtrC/NifA-type activator. Structural studies of TyrR identified three putative domains, including a domain containing binding sites for aromatic amino acids in the N-terminus, a central AAA+ ATPase domain, and a helix-turn-helix DNA binding motif domain in the C-terminus, which binds DNA sequences in promoter-operator regions. In addition, a bioinformatic analysis of promoter sequences in A . brasilense Sp7 genome revealed that putative promoters encompass one to three TyrR boxes in genes predicted to be regulated by TyrR. To gain insight into the phenotypes regulated by TyrR, a tyrR -deficient strain derived from A . brasilense Sp7, named A . brasilense 2116 and a complemented 2116 strain harboring a plasmid carrying the tyrR gene were constructed. The observed phenotypes indicated that the putative transcriptional regulator TyrR is involved in biofilm production and is responsible for regulating the utilization of D-alanine as carbon source. In addition, TyrR was observed to be absolutely required for transcriptional regulation of the gene dadA encoding a D-amino acid dehydrogenase. The data suggested that TyrR may play a major role in the regulation of genes encoding a glucosyl transferase, essential signaling proteins, and amino acids transporters.

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Changes in biofilm formation in the nonflagellated flhB1 mutant of Azospirillum brasilense Sp245

Cited by 20 publications

References 18 publications

Agriculturally important microbial biofilms: Present status and future prospects

Agriculturally important microbial biofilms: Present status and future prospects

Fourier Transform Infrared (FTIR) Spectroscopic Study of Biofilms Formed by the Rhizobacterium Azospirillum baldaniorum Sp245: Aspects of Methodology and Matrix Composition

TyrR is involved in the transcriptional regulation of biofilm formation and D-alanine catabolism in Azospirillum brasilense Sp7.

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