Changes in Brain Acetylcholinesterase and Oxidative Stress Biomarkers in African Catfish Exposed to Carbendazim

Ezeoyili, Ifeanyi Christian; Mgbenka, Bernard Obialo; Atama, Chinedu Ifeanyi; Ngwu, Godwin Ikechukwu; Madu, Josephine Chinenye

doi:10.1002/aah.10089

Cited by 27 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, these data differ from other studies that report suppression in AChE induced by increased cellular oxidative stress (Flora et al, 2013; Kayode et al, 2016; Bali et al, 2019; Ezeoyili et al, 2019; Pala et al, 2019; Ibrahim et al, 2020). In general, such studies argue that this can occur due to the deterioration of neurotransmission and oxidative damage.…”

Section: Resultscontrasting

confidence: 89%

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

View full text Add to dashboard Cite

The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpoles / 5 replicates of 10 animals) from species Physalaemus cuvieri (Leptodactylidae). After synthesis, purification, and characterization of peptides (PSDP2001, PSDP2002, PSDP2003) an aquatic contamination has been simulatedwith these peptides during 24 hours of exposure in two concentrations (100 and 500 ng/mL). The control group (“C”) was composed of tadpoles kept in polyethylene containers containing de-chlorinated water. Oxidative stress, antioxidant biomarkers and neurotoxicity activity were assessed. In both concentrations, PSPD2002 and PSPD2003 increased catalase and superoxide dismutase antioxidants enzymes activities, as well as oxidative stress (nitrite levels, hydrogen peroxide and reactive oxygen species). All three peptides also increased acetylcholinesterase activity in the highest concentration. These peptides showed molecular interactions in silico with acetylcholinesterase and antioxidant enzymes. Aquatic particle contamination of SARS-CoV-2 has neurotoxics effects in P. cuvieri tadpoles. These findings indicate that the COVID-19 can constitute environmental impact or biological damage potential.HIGHLIGHTSSARS-CoV-2 spike protein peptides (PSDP) were synthesized, purified, and characterized by solid phase peptide synthesis.PSDP peptides promoted REDOX imbalance and acute neurotoxicity in tadpoles (Physalaemus cuvieri)In silico studies have shown interactionsbetween peptides and acetylcholinesterase and antioxidant enzymesAquatic particle contamination of SARS-CoV-2 can constitute additional environmental damageGRAPHICAL ABSTRACT

show abstract

Section: Resultscontrasting

confidence: 89%

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…[ 7 ] The activity of acetylcholinesterase, an oxidative stress biomarker, was inhibited in the brain of the African cat fish Clarias gariepinus when exposed to sublethal concentrations of CBZ at 0.22–0.64 mg/L. [ 99 ] Thus, CBZ proves to be a strong oxidizing agent under in vitro‐vivo conditions.…”

Section: Introductionmentioning

confidence: 99%

Carbendazim toxicity in different cell lines and mammalian tissues

Sharma

Maheshwari

Khan

et al. 2022

J Biochem & Molecular Tox

View full text Add to dashboard Cite

The extensive production and use of harmful pesticides in agriculture to improve crop yield has raised concerns about their potential threat to living components of the environment. Pesticides cause serious environmental and health problems both to humans and animals. Carbendazim (CBZ) is a broad spectrum fungicide that is used to control or effectively kill pathogenic microorganisms. CBZ is a significant contaminant found in food, soil and water. It exerts immediate and delayed harmful effects on humans, invertebrates, aquatic animals and soil microbes when used extensively and repeatedly. CBZ is a teratogenic, mutagenic and aneugenic agent that imparts its toxicity by enhancing generation of reactive oxygen species generation. It elevates the oxidation of thiols, proteins and lipids and decreases the activities of antioxidant enzymes. CBZ is cytotoxic causing hematological abnormalities, mitotic spindle deformity, inhibits mitosis and alters cell cycle events which lead to apoptosis. CBZ is known to cause endocrine-disruption, embryo toxicity, infertility, hepatic dysfunction and has been reported to be one of the leading causes of neurodegenerative disorders. CBZ is dangerous to human health, the most common side effects upon chronic exposure are thyroid gland dysfunction and oxidative hepato-nephrotoxicity. In mammals, CBZ has been shown to disrupt the antioxidant defense system. In this review, CBZ-induced toxicity in different cells, tissues and organisms, under in vitro and in vivo conditions, has been systematically discussed.

show abstract

“…Furthermore, fatty acids were down‐regulated in the LT‐28d samples, whereas, triglyceride was up‐regulated, suggesting triglyceride accumulation in the liver and subsequently an increase in intrahepatic lipids. Lipid alterations in fish have been associated with xenobiotic exposure, including carbendazim (Ezeoyili et al, 2019; Jiang et al, 2015; Rebelo et al, 2020; Sakr & Shalaby, 2014; Ziarrusta et al, 2018). A study carried out on adult zebrafish showed that carbendazim exposure affected glycolipid metabolism and detoxification metabolism in the liver (Bao et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Untargeted Metabolomics Reveals Lipid Impairment in the Liver of Adult Zebrafish (Danio rerio) Exposed to Carbendazim

Costa

Chaib

Domingues

et al. 2023

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Carbendazim is a systemic fungicide used in several countries, particularly in Brazil. However, studies suggest that it is related to the promotion of tumors, endocrine disruption, and toxicity to organisms, among other effects. As a result, carbendazim is not allowed in the United States, Australia, and some European Union countries. Therefore, further studies are necessary to evaluate its effects, and zebrafish is a model routinely used to provide relevant information regarding the acute and long-term effects of xenobiotics. In this way, zebrafish water tank samples (water samples from aquari containing zebrafish) and liver samples from animals exposed to carbendazim at a concentration of 120 μg/L were analyzed by liquid chromatography coupled to high-resolution mass spectrometry, followed by multivariate and univariate statistical analyses, using the metabolomics approach. Our results suggest impairment of lipid metabolism with a consequent increase in intrahepatic lipids and endocrine disruption. Furthermore, the results suggest two endogenous metabolites as potential biomarkers to determine carbendazim exposure. Finally, the present study showed that it is possible to use zebrafish water tank samples to assess the dysregulation of endogenous metabolites to understand biological effects.

show abstract

Changes in Brain Acetylcholinesterase and Oxidative Stress Biomarkers in African Catfish Exposed to Carbendazim

Cited by 27 publications

References 41 publications

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Carbendazim toxicity in different cell lines and mammalian tissues

Untargeted Metabolomics Reveals Lipid Impairment in the Liver of Adult Zebrafish (Danio rerio) Exposed to Carbendazim

Contact Info

Product

Resources

About