Changes in cell surface charge and transmembrane potential accompanying neoplastic transformation of rat kidney cells

Price, Jonathan A.R.; Pethig, Ronald; Lai, Chi-Nan; Becker, Frederick F.; Gascoyne, Peter R. C.; Szent‐Györgyi, Albert

doi:10.1016/0005-2736(87)90031-9

Cited by 22 publications

(11 citation statements)

References 30 publications

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“…Interestingly, surface charge can change with the pathophysiological state of the cell, and this could affect galvanotaxis. Indeed, increased negative surface charge is known to be associated with malignant cancer cells (Abercrombie and Ambrose, 1962;Carter and Coffey, 1988;Carter et al, 1989;Price et al, 1987), and a surface charge effect could underlie the fast, cathodal galvanotaxis of rat prostate cancer cells ).…”

Section: The Influence Of Surface Chargementioning

confidence: 99%

Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease

Mycielska

Djamgoz

2004

Journal of Cell Science

331

300

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Endogenous direct-current electric fields (dcEFs) occur in vivo in the form of epithelial transcellular potentials or neuronal field potentials, and a variety of cells respond to dcEFs in vitro by directional movement. This is termed galvanotaxis. The passive influx of Ca2+ on the anodal side should increase the local intracellular Ca2+ concentration, whereas passive efflux and/or intracellular redistribution decrease the local intracellular Ca2+ concentration on the cathodal side. These changes could give rise to `push-pull' effects, causing net movement of cells towards the cathode. However, such effects would be complicated in cells that possess voltage-gated Ca2+ channels and/or intracellular Ca2+ stores. Moreover, voltage-gated Na+ channels, protein kinases, growth factors, surface charge and electrophoresis of proteins have been found to be involved in galvanotaxis. Galvanotactic mechanisms might operate in both the short term (seconds to minutes) and the long term (minutes to hours), and recent work has shown that they might be involved in metastatic disease. The galvanotactic responses of strongly metastatic prostate and breast cancer cells are much more prominent, and the cells move in the opposite direction compared with corresponding weakly metastatic cells. This could have important implications for the metastatic process and has clinical implications. Galvanotaxis could thus play a significant role in both cellular physiology and pathophysiology.

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Section: The Influence Of Surface Chargementioning

confidence: 99%

Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease

Mycielska

Djamgoz

2004

Journal of Cell Science

331

300

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“…In an earlier report, we demonstrated that TMP, EPM, and redox activities in a family of cultured rat kidney cell lines paralleled the state of transformation of the cells (Price et al, 1987). We have now extended our studies to two Friend murine erythroleukemia (MEL) cell lines.…”

mentioning

confidence: 82%

“…Hours of HMBA Treatment timed in order to derive an EPM value, and each experimental condition was repeated on least three iusually five or more) occasions. Zeta potentials were calculated from the electrophoretic mobility data using the Helmholtz-Smoluchowski equation (see Price et al, 1987). While the EPM of cells that were maintained a t 37°C prior to measurement tended to be erratic, that of cells stored at ice temperature for a t least 15 minutes prior to measurement was reproducible.…”

Section: Electrophoresis Measurementsmentioning

confidence: 99%

“…The bioelectrical properties of the plasma membranes of mammalian cells have frequently been observed to change when cells undergo neoplastic transformation; it has been suggested that such altered activities may be related to the genesis and maintenance of the malignant state (Bingelli and Cameron, 1980;Boonstra et al, 1981;Cone, 1974;Lai et al, 1984;Price et al, 1987). A strong correlation was found between transmembrane potential (TMP) and commitment to division in cells of 56 different origins (Binggeli and Weinstein, 19861, and cell surface charge has been found to parallel the state of transformation in some cell types.…”

mentioning

confidence: 99%

“…A strong correlation was found between transmembrane potential (TMP) and commitment to division in cells of 56 different origins (Binggeli and Weinstein, 19861, and cell surface charge has been found to parallel the state of transformation in some cell types. Because the membrane outer surface usually carries a greater excess of fixed negative charge than the inside surface (Pethig, 1976), the voltage acting directly across the plasma membrane may differ by as much as 15 mV from the TMP determined by electrode or potential-sensitive dye methods (Price et al, 1987). Thus the electric field applied to transmembrane proteins is a function of both TMP and surface charge.…”

mentioning

confidence: 99%

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Alterations in electrophoretic mobility, diaphorase activity, and terminal differentiation induced in murine erythroleukemia lines by differentiating agents

Gascoyne

Becker

1990

Journal Cellular Physiology

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The electrophoretic mobilities (EPMs) and semiquinone reductase activities of two clones of Friend murine erythroleukemia (MEL) cells were investigated as a function of treatment with the inducing agents dimethylsulfoxide (DMSO) and hexamethylene bisacetamide (HMBA). As reported previously by others, the inducible clone DS19 lost its ability to grow in soft agar and expressed hemoglobin as judged by benzidine/H2O2 staining after 96 hours of treatment with 1% DMSO or 4 mM HMBA. In addition, its EPM fell by 14%, its semiquinone reductase activity by 40%, and its mean diameter by 10%. The second clone, R1, retained its ability to grow in soft agar and lacked hemoglobin expression after treatment with HMBA and DMSO, characterizing it as noninducible. However, R1 did demonstrate alterations in EPM, semiquinone reductase activity, and cell diameter that closely paralleled those found in DS19. Such responses were not seen in three non-MEL cell lines exposed to HMBA or DMSO, suggesting that clone R1 responded to these inducing agents in a cell-line specific manner but that its ability to complete the sequences necessary for differentiation may be blocked at an unknown point distal to the block characteristic of untreated cells. The data show that while a reduction in EPM, semiquinone reductase activity, and cell diameter accompany induced differentiation in MEL cells, such changes can occur in the absence of a commitment to terminal differentiation.

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How does Dielectrophoresis Differ from Electrophoresis?

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Changes in cell surface charge and transmembrane potential accompanying neoplastic transformation of rat kidney cells

Cited by 22 publications

References 30 publications

Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease

Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease

Alterations in electrophoretic mobility, diaphorase activity, and terminal differentiation induced in murine erythroleukemia lines by differentiating agents

How does Dielectrophoresis Differ from Electrophoresis?

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