Changes in collagen cross-link ratios in bone and urine of guinea pigs fed graded dietary vitamin C: a functional index of vitamin C status

Tsuchiya, Harumi; Bates, Christopher J.

doi:10.1016/s0955-2863(98)00039-4

Cited by 13 publications

(6 citation statements)

References 26 publications

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“…As in our first study (Tsuchiya & Bates, 1997), the concentration of hydroxyproline per unit wet weight of femur did not differ significantly between feed groups. In our second study (Tsuchiya & Bates, 1998), a small and unexpected increase in femur shaft hydroxyproline per unit wet weight was seen when the vitamin C content of the diet was decreased. Taking all three studies together, it appears that femur shaft collagen, unlike dorsal skin collagen, does not fall precipitously in animals that are deprived of vitamin C, nor is it significantly affected in animals whose growth is retarded by total food restriction.…”

Section: Discussionmentioning

confidence: 58%

“…vitamin C inadequacy) in man. As noted previously (Tsuchiya & Bates, 1998), the high sensitivity of bone and urinary collagen crosslink ratios to moderate degrees of vitamin C-restriction in guinea-pigs helps to complete the sequence from a pathological lesion (scurvy), via a defined biochemical reaction (altered cross-link ratios), to a potential non-invasive biochemical probe of vitamin C status.…”

Section: Discussionmentioning

confidence: 73%

“…Each pyridinoline residue is derived from three hydroxylysine residues, whereas deoxypyridinoline is derived from two hydroxylysine and one lysine residue. If lysyl hydroxylase activity is reduced by lack of ascorbate, the value of the deoxypyridinoline crosslinks:pyridinoline cross-links ratio increases (Tsuchiya & Bates, 1997;Tsuchiya & Bates, 1998). Unlike hydroxyproline (Schwarz et al 1987), hydroxylysine is apparently not required for the stabilization of the collagen triple helix.…”

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confidence: 99%

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Comparison of vitamin C deficiency with food restriction on collagen cross-link ratios in bone, urine and skin of weanling guinea-pigs

Tsuchiya

Bates

2003

Br J Nutr

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Mild-to-moderate vitamin C depletion in weanling guinea-pigs affects pyridinoline:deoxypyridinoline (collagen cross-link) ratios in femur shaft and urine, attributed to impairment of hydroxylation of collagen lysine. We investigated: (1) whether the picture at two time points is compatible with progressive accumulation of abnormal collagen; (2) whether any changes are seen in skin, where little deoxypyridinoline occurs; (3) whether total food restriction has similar effects. Male weanling Dunkin-Hartley guinea-pigs were fed diets containing either 0·5 (vitamin C-restricted) or 160·0-320·0 (vitamin C-adequate) mg vitamin C/d. Two groups receiving the vitamin C-adequate diet received it ad libitum. Two other groups received the vitamin C-adequate diet in a restricted amount, limited to that which permitted nearly the same growth rate as in the vitamin C-restricted groups. Animals were fed for 4 or 8 weeks; urine was collected, and vitamin C and collagen indices were measured. In the femur shaft, the hydroxyproline content per unit weight was unaffected by vitamin C restriction or by total food restriction. Deoxypyridinoline was increased and the pyridinoline:deoxypyridinoline ratio was decreased in vitamin C-restricted groups, but not in food-restricted groups. Changes in the value of the ratio were greater after 8 than after 4 weeks. Urine indices mirrored bone indices. In skin, the main effect of vitamin C restriction was to reduce hydroxyproline. Here, the cross-link ratios changed less markedly than in bone, and there was less deoxypyridinoline. We conclude that the picture at two time points is compatible with a progressive accumulation of pyridinoline-enriched collagen in vitamin C-deprived animals, that the picture in skin differs from that of bone and urine, and that cross-link changes are not produced by total food restriction.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 73%

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confidence: 99%

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Comparison of vitamin C deficiency with food restriction on collagen cross-link ratios in bone, urine and skin of weanling guinea-pigs

Tsuchiya

Bates

2003

Br J Nutr

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“…[12][13][14] L-Ascorbic acid (vitamin C), a representative water-soluble vitamin, has a variety of biological, pharmaceutical and dermatological functions; it promotes collagen biosynthesis, provides photoprotection, causes melanin reduction, scavenges free radicals, and enhances the immunity (antiviral effect). [15][16][17][18][19] These properties are closely related to the well-known antioxidant properties of this compound. However, the transdermal permeability of vitamin C is low.…”

Section: Introductionmentioning

confidence: 82%

Enhancement of skin permeation of vitamin C using vibrating microneedles

Lee

Baek

Kwag

et al. 2017

Transl Clin Pharmacol

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This study was performed to evaluate the use of vibrating microneedles for the transdermal delivery of vitamin C. The microneedles were designed to vibrate at three levels of intensity. In vitro permeation by vitamin C was evaluated according to the specific conditions such as vibration intensity (levels 1, 2 and 3), application time (1, 3, 5, 7 and 10 min), and application power (500, 700 and 1,000 g). The highest permeation of vitamin C was observed at level 3 of vibration intensity, 5 min of application, and 1,000 g of application power. Vitamin C gel showed no cytotoxic effect against Pam212 cells or skin irritation effects. A pharmacokinetic study of the gel in rats was conducted under optimized conditions. The AUC 0 -∞ and C max increased 1.35-fold and 1.44-fold, respectively, compared with those after vitamin C gel without application with vibrating microneedles. The present study suggests that vibrating microneedles can be used to facilitate the skin permeability of vitamin C under optimal conditions.

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“…This vitamin is a cofactor for two enzymes, lysyl and prolyl hydroxylases, which are related to collagen hydroxylation. The function of AA as a cofactor for these enzymes is to avoid their iron atom oxidation and, consequently, protect them from autoinactivation (Azulay et al 2003, Tuero 2000, Parsons et al 2006, Murad et al 1981, Tsuchiya and Bates 1998, Senturk et al 2004. AA also enhances the collagen synthesis because it increases the gene expression of fibroblasts (Tuero 2000, Senturk et al 2004.…”

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confidence: 99%

Histological evaluation of the periodontal ligament from aged wistar rats supplemented with ascorbic acid

Zanoni

Lucas

Trevizan

et al. 2013

An. Acad. Bras. Ciênc.

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Ascorbic acid (AA) is able to neutralize reactive oxygen species and is essential for collagen synthesis. In aging process oxidative stress is elevated. This study aims to investigate the effects of AA supplementation on the periodontal ligament (PL) of rats during aging. Twenty five rats were used and divided into groups: J90 (90-dayold control), E345 (345-day-old control), E428 (428-day-old control), EA345 (345-day-old supplemented with AA from 90-day-old on) and EA428 (428-day-old supplemented with AA from 90-day-old on). We analyzed the thickness, density of fibroblasts and blood vessels and collagen fibers types in the PL. In group J90 there was predominantly type III collagen fibers (87.64%). In animals supplemented with AA, the area filled by type I fibers (group EA345: 65.67%, group EA428: 52.23%) was higher than type III fibers. PL in group EA428 was thicker than the one observed in group E428 (P < 0.05). During natural aging process, AA promoted the maturation of collagen fibers and enhanced angiogenesis in periodontal ligament. One can conclude that the supplementation with AA represented a beneficial factor for the development of PL in aged rats.

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Changes in collagen cross-link ratios in bone and urine of guinea pigs fed graded dietary vitamin C: a functional index of vitamin C status

Cited by 13 publications

References 26 publications

Comparison of vitamin C deficiency with food restriction on collagen cross-link ratios in bone, urine and skin of weanling guinea-pigs

Comparison of vitamin C deficiency with food restriction on collagen cross-link ratios in bone, urine and skin of weanling guinea-pigs

Enhancement of skin permeation of vitamin C using vibrating microneedles

Histological evaluation of the periodontal ligament from aged wistar rats supplemented with ascorbic acid

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