Changes in distribution of extracellular matrix proteins during wound repair in corneal endothelium.

Abstract

The distribution of fibronectin (FN) and laminin (LM) in non-injured and injured rat corneal endothelium in vivo was investigated by light microscopy using immunoperoxidase cytochemistry. In non-injured tissues, both FN and LM have distinct pericellular staining patterns and exhibit some diffuse cytoplasmic staining. After a circular freeze injury, cells migrating into the wound area at 24 hr lack the characteristic pericellular staining observed in non-injured cells but show cytoplasmic staining for both extracellular matrix glycoproteins. Endothelial cells on the periphery of such preparations do not partake in wound repair and retain their pericellular staining patterns. Forty-eight hours after injury, cells have filled in the wound area but are disorganized. They display intracellular FN and LM staining but do not demonstrate any pericellular staining. When observed 10 days after injury, a uniform monolayer has formed but neither FN nor LM is detected pericellularly. By 14 days post injury, endothelial cells in the wound area display pericellular FN patterns but not LM patterns. This may reflect differences in the function of each glycoprotein in maintaining the attachment of the endothelium to Descemet's membrane.

Poloxamines for Deswelling of Organ-Cultured Corneas

“…Cell migration and spreading is the major mechanism in corneal endothelial wound healing. It is driven by soluble growth factors and cytokines released concordantly into the injured site [42,43] leading to reorganization of actin and microtubule cytoskeletons [44] and reduced cell adhesion [45]. Poloxamines may globally improve endothelial cells’ metabolic functions by better preserving membrane integrity and promote wound healing.…”

Differential adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells in primary culture.

“…To ascertain whether modulation of the expression of fibronectin (FN) during migration (15,16) in parallel. IF specimens were observed on a microscope equipped with epifluorescence illumination (Axiophot Zeiss) and selected areas were analyzed on a confocal laser system (MRC 600; Bio-Rad Laboratories, Richmond, CA) connected to the Axiophot microscope.…”

Expression of SV40 virus large T antigen by recombinant adenoviruses activates proliferation of corneal endothelium in vitro.

“…A characteristic hexagonal shape and circumferential peripheral ring pattern ofF-actin staining have been seen in human, rabbit, bovine corneal endothelium (Fig. 3A) (20,21 ), as these morphological characteristics are not seen in keratocytes isolated from the cornea (Fig. 3B).…”

“…The cells were passaged once and plated onto coverslips for infection. These cells were deemed to be endothelial by well-established morphologic criteria under phase-contrast microscopy and by their F-actin staining pattern (20,21 ). HCE were isolated in the same fashion as described above from a 15-mo-old donor cornea (obtained from the San Diego Eye Bank) and pooled from the left and right corneas of a 66-yr-old donor unsuitable for human transplantation (donated by the San Diego Eye Bank).…”