Changes in Electrophoretic Pattern of Ribonucleases During Aging of Potato Tuber Slices

Isola, María Clara; Franzoni, Luis

doi:10.1016/s0044-328x(81)80160-2

Cited by 11 publications

(2 citation statements)

References 12 publications

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“…Acidic, neutral salt-stimulated and neutral saltinhibited RNases (Blank and McKeon 1991) were estimated by measuring acid-soluble nucleotides with Torula yeast RNA (10 μg cm -3 ) as substrate according to Isola and Franzoni (1981) with minor modification . The buffer in the reaction mixture was specific for each different class of RNase as described below.…”

Section: Rnases Extraction and Enzymatic Assaymentioning

confidence: 99%

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

Spanò¹,

Buselli²,

Grilli³

2008

Biologia plant.

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Acidic and neutral ribonucleases (RNases) were studied in embryos of Triticum durum cv. Cappelli and the effects of abscisic acid (ABA) and gibberellic acid (GA 3 ) were analysed. RNases activities increased during germination and were comparable in dormant and non-dormant embryos imbibed for 24 h. ABA generally inhibited ribonucleolytic activities, while GA 3 only affected dormant embryos. To assess whether changes in RNase activities during germination or following hormonal treatment required new transcriptional or translational action, cycloheximide or cordycepin were used. The action of inhibitors of acidic RNase activity was found only in non-dormant-embryos. Findings obtained in the present work concur with a change of the ribonucleolytic pattern in the shift from dormant to non dormant metabolism.

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Section: Rnases Extraction and Enzymatic Assaymentioning

confidence: 99%

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

Spanò¹,

Buselli²,

Grilli³

2008

Biologia plant.

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“…Similarly, Ogawa and Uritani (1970) found an increase in the isoenzymes of peroxidase . The situation is also very similar in the case of mechanical injury or other stress effects (Isola andFranzoni 1981, Wyen et al . 1972) .…”

Section: Discussionmentioning

confidence: 89%

Inverse Correlation between Growth and Degrading Enzyme Activity of Seedlings after Gamma and Neutron Irradiation of Pea Seeds

Bagi

Bornemisza-Pauspertl

Hidvégi

1988

International Journal of Radiation Biology

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The effect of gamma-, 14 MeV neutron- and fission neutron irradiation was investigated on the growth rate and degrading enzyme activities of pea seedlings. Both dormant pea seeds and 4-day-old growing seedlings were used for the experiments. Depending on the gamma dose between 15 and 300 Gy the height of pea seedlings was found shorter, and parallel with this the endogenous RNase and peroxidase activities were higher than in the unirradiated controls. Seedlings proved to be more sensitive by about one order of magnitude than seeds. Irradiation of seeds between 5 and 10 Gy slightly enhanced the growth rate of seedlings (10 per cent) and parallel with this, the RNase activity measured was lower than that in the controls. On irradiation of seedlings with 14 MeV neutrons the growth inhibition and RNase activity enhancement was only 1.3 times more effective than in the case of irradiation of seeds. The following RBE values were obtained after irradiation of seeds, related to the biological effect of gamma rays: in growth inhibition, 6 for 14 MeV neutrons and 12 for fission neutrons, and the enhancement of two enzyme activities was 15-30 for 14 MeV neutrons and 45-58 for fission neutrons. In the case of seedling irradiation with 14 MeV neutrons the RBE was 1.0 for growth inhibition and between 3 and 6 for enhancement of enzyme activity. The isoenzyme pattern of RNase also changed: two isoenzymes became predominant after the gamma irradiation of seeds, characterized by molecular weights of 21,000 and 30,000, respectively. As a result of enhanced RNase activity, the degradation of longer polysomes to monomeric ribosomes occurred. Thus after ionizing irradiation of pea seeds and seedlings an inverse correlation was found between the growth rate of pea seedlings and the activities of degrading enzymes.

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Applications of Isozymes in Plant Breeding

Weeden

1989

Plant Breeding Reviews

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Changes in Electrophoretic Pattern of Ribonucleases During Aging of Potato Tuber Slices

Cited by 11 publications

References 12 publications

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

Inverse Correlation between Growth and Degrading Enzyme Activity of Seedlings after Gamma and Neutron Irradiation of Pea Seeds

Applications of Isozymes in Plant Breeding

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