No abstract

After imbibition of peanut seeds, the concentration of free amino acids in cotyledons increases probably due to hydrolysis of reserve proteins. That increase was accompanied by a stimulation of aspartate aminotransferase (AAT, EC 2.6.1.1) activity especially pronounced between first and sixth day of imbibition. Peanut cotyledons contained several AAT isoforms which could be visualized after polyacrylamide gel electrophoresis (PAGE). Native PAGE of extracts from cotyledons of dry seeds demonstrate three active bands. The intensity of these bands increased reaching a maximum after 6th day of imbibition. An additional band appears transientely on 6th day, but is not visible after 14 days of incubation. A fastest band appears on this latter day. If germination takes place under hypersalinity conditions, the AAT activity severely diminishes and the tree amino acid concentration raises. The possibilities of different types of AAT regulation are discussed.

No abstract

In‐8‐day‐old rats the higher rate of DNA replication in cerebellum than in cerebrum is accompanied by an enhanced synthesis of nuclear proteins. The greatest difference between the incorporation of tritiated leucine into proteins of cerebral and cerebellar cell nuclei occurs in the acid‐soluble deoxyribonucleoproteins. However, the specific radioactivity of the acidic deoxyribonucleoproteins is similar in both tissues. The relative content of these proteins and the activity of the RNA polymerase is higher in cerebrum than in cerebellum. The results suggest that in the cerebrum of young rats these proteins are mainly concerned with the regulation of RNA synthesis.

No abstract