Changes in ethylene responsiveness of senescence‐related genes during carnation flower development

Abstract

Changes in ethylene responsiveness of senescence-related (SR) genes in carnation (Dianthus caryophyllus cv. White Sim) petals were investigated during flower development. Dose-response and time-response analysis of SR gene expression indicate that SR genes can be divided into two groups according to their response to ethylene. The ethylene biosynthetic genes, ACC synthase and ACC oxidase represent one group. They show a marked delay of 6 and 9 h in mRNA accumulation in response to ethylene and their apparent dissociation constants of the response (K r ) at open flower stage of development are 17.20 and 1.76 ml l ª1 ,

Expression and Regulation of Senescence-related Genes in Carnation Flowers with Low Ethylene Production during Senescence

“…Many senescence-related (SR) genes, including those encoding these enzymes, have been cloned from carnation petals. The expression levels of DcCP1 (previously known as pDCCP1, isolated by Jones et al, 1995), β-galactosidase (DcbGal; previously called SR12) (Lawton et al, 1989), glutathione-S-transferase (DcGST1), and lipase (DcLip) increased during senescence and were upregulated by ethylene treatment (Verlinden et al, 2002). In contrast, levels of DcCPIn expression decreased during senescence and were downregulated by ethylene (Sugawara et al, 2002).…”

“…DcCP1, DcbGal, DcGST1, and DcLip are upregulated by exogenous ethylene (Fig. 7A, D-F) (Hong et al, 2000;Jones et al, 1995;Verlinden et al, 2002). Genomic analysis for characteristic ciselement motifs of promoter sequences identified the ethylene-responsive element (ERE) of carnation or ERElike sequences in the 5'-upstream regions of these genes (Itzhaki and Woodson, 1993;Itzhaki et al, 1994;Kosugi et al, 2007;Verlinden et al, 2002).…”

Flower senescence: some molecular aspects

“…Tieman et al (2001) have demonstrated that reduced EIL expression in tomato (LeEIL1) affects ethylene responses, including leaf epinasty, flower abscission, flower senescence and fruit ripening. However, the expression of EIN3-like gene (EIL1) in Nicotiana sylvestris plants has not been found to consistently alter the progression of senescence Suzuki et al 1998;Li and Guo 2007), which is evident by the presence of ethylene responsive elements (eRes) in some senescence-related genes (SR5, SR9 and SR12) and related transcription factors (Hunter and Reid 2001;verlinden et al 2002). eRFs are plant-specific AP2/EREBP-type transcription factors, characterized by the presence of a highly conserved DNA-binding domain (the ERF domain consisting of 58 or 59 amino acids) and regulate gene expression by binding specifically to the 11 bp GCC box of the ethylene responsive element of senescence-related genes (Ohme-Takagi and Shinshi 1995; Hao et al 1998;Riechmann and Meyerowitz 1998;Yang et al 2011).…”

Regulation of Ethylene- and Senescence-related Genes in Pot Carnation Flowers during Flower Senescence

“…85 (3): 254-263. 2016. quences in the 5'-upstream promoter regions of some SR genes such as DcCP1, DcGST1, and DcbGal (Itzhaki et al, 1994;Kosugi et al, 2007;Solano et al, 1998;Verlinden et al, 2002). DcEIL3, which is regulated by ethylene and sugar treatment, may regulate the expression of DcGST1 (SR8) and DcbGal (SR12) (Iordachescu and Verlinden, 2005).…”