Changes in expression of N-methyl-d-aspartate receptor subunits in the rat neostriatum after a single dose of antisense oligonucleotide specific for N-methyl-d-aspartate receptor 1 subunit

Lai, Suk King; Wong, Chris K C; Yang, M. S.; Yung, Kin Lam

doi:10.1016/s0306-4522(00)00152-4

Cited by 38 publications

(28 citation statements)

References 26 publications

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“…Data are presented as relative fold change (RFC) to SALcontrols. Primers were derived from previously published reports (Barbon et al, 2006;Lai et al, 2000) and primer sequences can be found in Table 1.…”

Section: Rt-pcr For Glutamate Receptor Mrnamentioning

confidence: 99%

Viral-like brain inflammation during development causes increased seizure susceptibility in adult rats

Galic

Riazi

Henderson

et al. 2009

Neurobiology of Disease

101

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Viral infections of the CNS and their accompanying inflammation can cause long-term neurological effects, including increased risk for seizures. To examine the effects of CNS inflammation, we infused polyinosinic: polycytidylic acid, intracerebroventricularly to mimic a viral CNS infection in 14 day-old rats. This caused fever and an increase in the pro-inflammatory cytokine, interleukin (IL)-1β in the brain. As young adults, these animals were more susceptible to lithium-pilocarpine and pentylenetetrazol-induced seizures and showed memory deficits in fear conditioning. Whereas there was no alteration in adult hippocampal cytokine levels, we found a marked increase in NMDA (NR2A and C) and AMPA (GluR1) glutamate receptor subunit mRNA expression. The increase in seizure susceptibility, glutamate receptor subunits, and hippocampal IL-1β levels were suppressed by neonatal systemic minocycline. Thus, a novel model of viral CNS inflammation reveals pathophysiological relationships between brain cytokines, glutamate receptors, behaviour and seizures, which can be attenuated by anti-inflammatory agents like minocycline.

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Section: Rt-pcr For Glutamate Receptor Mrnamentioning

confidence: 99%

Viral-like brain inflammation during development causes increased seizure susceptibility in adult rats

Galic

Riazi

Henderson

et al. 2009

Neurobiology of Disease

101

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“…Oligonucleotide transcripts were designed on the basis of the known sequences of rat NR1 [26,29] . All transcripts used in the present study contained 20 phosphorothiolated nucleotides and were purchased commercially (Gibco).…”

Section: Oligonucleotide Treatmentsmentioning

confidence: 99%

“…All transcripts used in the present study contained 20 phosphorothiolated nucleotides and were purchased commercially (Gibco). ANR1 transcripts were proven by our laboratory to enable specifi c knockdown of NR1 protein expression in rat in vivo [26,34] . The sequences of the oligos were as follows: 5 -G CAG GTG CAT GGT GCT CAT G-3 (ANR1), 5 -C ATG AGC ACC ATG CAC CTG C-3 (NR1 sense: SNR1), 5 -A ACG TGA GCG TGG TCC TAG T-3 (randomized sequence based on the NR1 sequence: RNR1).…”

Section: Oligonucleotide Treatmentsmentioning

confidence: 99%

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Ablation of Gene Expression ofN-Methyl-<i>D</i>-Aspartate Receptor One by Antisense Oligonucleotides in Striatal Neurons in Culture

Lui

Yeung²,

Yung³

et al. 2005

Neurosignals

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In the present study, a twenty-mer antisense oligonucleotide specific for N-methyl-D-aspartate receptor one (ANR1) was applied to striatal neurons in primary cell culture. The ANR1 was found to be specific and nontoxic. Significant reductions in expression of NR1 mRNA and proteins were resulted after a single dose of ANR1 transcripts. Interestingly, there were reductions in total NR1 proteins but two phosphorylated forms of NR1 proteins at serine 896 and 897 residues were not reduced. There was also no change in the pattern of distribution of NR1 immunoreactivity in the striatal neurons. In addition, significant reductions of NMDA-mediated peak inward current were found after application of a higher concentration of ANR1 (20–100 µM) by patch clamp recordings. The present results indicate that ANR1 is a useful agent in reducing NMDA receptor functions. The present data thus provide detailed cellular and molecular mechanisms to explain our previous findings of amelioration of motor symptoms in a rat model of Parkinson’s disease. More importantly, application of ANR1 was also found to display neuroprotective effects of striatal neurons against NMDA-induced excitotoxic cell death. The findings have implications in development of new approach in prevention of cell death in neurodegenerative diseases and new treatments for these diseases.

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“…The three groups of rats (see above) were decapitated and the striatal tissues were dissected as previously described [21][22][23]. At least 5 rats were used in each group and tissues from each rat were obtained for analysis.…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Differential Expression of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole-Propionate Glutamate Receptors in the Rat Striatum during Postnatal Development

et al. 2003

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Alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propio- nate (AMPA)-type glutamate receptors (GluR1–4) are one of the most important ionotropic glutamate receptors in the striatum, a key region of the basal ganglia. The present study investigated the trend of developmental expression of AMPA receptor subunits in the striatum of rats in different developmental stages, i.e., at postnatal day 7 (PND7), PND21 and adult. Perfuse-fixed striatal sections were used. The expression of AMPA subunits was studied by immunofluorescence and reverse transcriptase-polymerase chain reaction (RT-PCR). RT-PCR revealed that the levels of expression of the GluR1 and GluR3 mRNAs were the same among the age groups. The level of GluR2 mRNA was highest in PND21 rats and lowest in adult. The highest level of GluR4 mRNA was detected in rats at PND7. Similar trends of GluR1, GluR2 and GluR2/3 immunoreactivity expression were observed using commercially available specific antibodies. In addition, a large proportion of parvalbumin-immunoreactive GABAergic interneurons in the striatum were found to display GluR1 immunoreactivity in PND21 and adult rats. In contrast, most of the choline acetyltransferase-immunoreactive cholinergic interneurons were found to display GluR2 immunoreactivity but less GluR1 and no GluR2/3 immunoreactivity in PND21 and adult rats. The present study suggests that there is a distinct pattern of expression of AMPA-type receptor mRNAs and proteins in the rat striatum at different stages of development.

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Changes in expression of N-methyl-d-aspartate receptor subunits in the rat neostriatum after a single dose of antisense oligonucleotide specific for N-methyl-d-aspartate receptor 1 subunit

Cited by 38 publications

References 26 publications

Viral-like brain inflammation during development causes increased seizure susceptibility in adult rats

Viral-like brain inflammation during development causes increased seizure susceptibility in adult rats

Ablation of Gene Expression ofN-Methyl-<i>D</i>-Aspartate Receptor One by Antisense Oligonucleotides in Striatal Neurons in Culture

Differential Expression of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole-Propionate Glutamate Receptors in the Rat Striatum during Postnatal Development

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