Changes in Friend murine erythroleukaemia cell membranes during induced differentiation determined by electrorotation

Wang, Xiaobo; Huang, Ying; Gascoyne, Peter R. C.; Becker, Frederick F.; Hölzel, Ralph; Pethig, Ronald

doi:10.1016/0005-2736(94)90170-8

Cited by 182 publications

(203 citation statements)

References 37 publications

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“…Knowing the electrode polarization ratio and by using Eq. 4, we calculated the cell levitation height as a function of frequency, using our earlier reported size and dielectric parameters for DS19 cells based on electrorotation [13], r = 5.41 μm, ε mem = 9.0ε 0 , σ men = 3.5×10 -7 S/m, ε int = 87ε 0 and σ int = 0.89 S/m, and we give the result for the conditions ε m = 80ε 0 , σ m = 56 mS/m and 5 V p-p in Fig. 7.…”

Section: Ac Field Induced Membrane Potential Did Not Cause the Observmentioning

confidence: 99%

“…Alternatively, if the field is rotating, the interaction between the field and the induced dipole will make the particle rotate (ROT: electrorotation) [9][10][11]. The techniques of DEP and ROT can be used noninvasively to characterize the dielectric properties of individual biological cells and have led to new methods for cell characterization, manipulation and, particularly, cell sorting [12][13][14][15][16][17][18][19][20][21][22][23][24]. As a cell sorting method, these techniques have the advantage of being simple, easy to use and non-invasive [22,23].…”

Section: Introductionmentioning

confidence: 99%

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Role of peroxide in AC electrical field exposure effects on Friend murine erythroleukemia cells during dielectrophoretic manipulations

Wang

Yang

Gascoyne

1999

Biochimica Et Biophysica Acta (BBA) - General Subjects

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The effects of AC field exposure on the viability and proliferation of mammalian cells under conditions appropriate for their dielectrophoretic manipulation and sorting were investigated using DS19 murine erythroleukemia cells as a model system. The frequency range 100 Hz-10 MHz and medium conductivities of 10 mS/m, 30 mS/m and 56 mS/m were studied for fields generated by applying signals of up to 7V peak to peak (p-p) to a parallel electrode array having equal electrode widths and gaps of 100 μm. Between 1 kHz and 10 MHz, cell viability after up to 40 min of field exposure was found to be above 95% and cells were able to proliferate. However, cell growth lag phase was extended with decreasing field frequency and with increasing voltage, medium conductivity and exposure duration. Modified growth behavior was not passed on to the next cell passage, indicating that field exposure did not cause permanent alterations in cell proliferation characteristics. Cell membrane potentials induced by field exposure were calculated and shown to be well below values typically associated with cell damage. Furthermore, medium treated by field exposure and then added to untreated cells produced the same modifications of growth as exposing cells directly, and these modifications occurred only when the electrode polarization voltage exceeded a threshold of ~0.4 V p-p. These findings suggested that electrochemical products generated during field exposure were responsible for the changes in cell growth. Finally, it was found that hydrogen peroxide was produced when sugar-containing media were exposed to fields and that normal cell growth could be restored by addition of catalase to the medium, whether or not field exposure occurred in the presence of cells. These results show that AC fields typically used for dielectrophoretic manipulation and sorting of cells do not damage DS19 cells and that cell alterations arising from electrochemical effects can be completely mitigated.

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Section: Ac Field Induced Membrane Potential Did Not Cause the Observmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of peroxide in AC electrical field exposure effects on Friend murine erythroleukemia cells during dielectrophoretic manipulations

Wang

Yang

Gascoyne

1999

Biochimica Et Biophysica Acta (BBA) - General Subjects

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“…89,90 Generally, the electrical properties of a plasma membrane are affected by the membrane morphology, lipid bilayer composition and thickness, and embedded proteins. [91][92][93] Electrical properties of the cytoplasm are influenced by the intracellular structures and physiological conditions (e.g., nucleus-to-cytoplasm ratio and ion concentrations inside the cell). [94][95][96] Early techniques were only capable of measuring the average electrical properties of a cell population.…”

Section: Electrical Characterization Techniquesmentioning

confidence: 99%

“…By fitting the rotation spectra, specific membrane capacitance, cytoplasm conductivity and cytoplasm permittivity values are determined. 92,114,115 Fig. 3(b) illustrates the working mechanism of a microfluidic electro-rotation device.…”

Section: Electrorotationmentioning

confidence: 99%

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Recent advances in microfluidic techniques for single-cell biophysical characterization

et al. 2013

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A novel dielectrophoresis-based device for the selective retention of viable cells in cell culture media

Docoslis

Kalogerakis

Behie

et al. 1997

Biotechnol. Bioeng.

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Changes in Friend murine erythroleukaemia cell membranes during induced differentiation determined by electrorotation

Cited by 182 publications

References 37 publications

Role of peroxide in AC electrical field exposure effects on Friend murine erythroleukemia cells during dielectrophoretic manipulations

Role of peroxide in AC electrical field exposure effects on Friend murine erythroleukemia cells during dielectrophoretic manipulations

Recent advances in microfluidic techniques for single-cell biophysical characterization

A novel dielectrophoresis-based device for the selective retention of viable cells in cell culture media

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