Changes in G1-phase populations in human glioblastoma and neuroblastoma cell lines influence p(66)/Be neutron-induced micronucleus yield

Cell death induced in a human glioblastoma cell line by p(65)+Be neutrons combined with cisplatin

“…However, these cancer cells are efficiently killed by carbon ions (Iwadate et al, 2001;Tsuchida et al, 1998). Interestingly, it has been recently reported that the amount of micronuclei induced in glioblastoma cell lines by neutron, but not by photon, was dependent on the intrinsic ability of these cells to activate a G1-phase arrest (Akudugu et al, 2004). On the other side, the activation of the G1 checkpoint has been shown to be dependent on the type of specific DNA damage inducer.…”

“…In the present study, we have investigated the consequences of a treatment combining fast neutrons with cisplatin on the growth and cell death induction in U-87, a human glioblastoma cell line. Indeed, these tumors are resistant to photon but sensitive to fast neutrons (Akudugu et al, 2004). Therefore, they provide ideal model system to explore the cytotoxic response to high-LET radiation.…”

Erythropoietin Augments Survival of Glioma Cells After Radiation and Temozolomide

“…Irradiation was followed by 24 h concomitant exposure to TMZ (0, 25, 50, 100, or 200 mM) and EPO. Subsequently, medium was replaced with fresh medium for the remaining part of the clonogenic assay (38). For that, 200 cells/well were seeded in six-well plates and incubated at 37 C in 7.5% CO 2 humidified atmosphere.…”