Changes in Gene Expression during Leaf Abscission

Roberts, Jeremy A.; Taylor, Jane; Coupe, Simon A.; Harris, Neil; Webb, Simon Tj

doi:10.1007/978-94-017-1003-9_64

Cited by 6 publications

(1 citation statement)

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“…Plant cell walls are rich in celluloses, hemicelluloses, xyloglucans and pectins. Degradation of the AZ cells requires the activity of multiple cell wall remodeling enzymes, including polygalacturonase (PG) (Kalaitzis et al ., 1997; Ogawa et al ., 2009), cellulase (CEL) (Roberts et al ., 1993; Li et al ., 2019), pectin methylesterases (Lashbrook and Cai, 2008), β‐galactosidase (Wu and Burns, 2004; Lashbrook and Cai, 2008; Agusti et al ., 2009; Gao et al ., 2019), xyloglucan endotransglucosylase/hydrolase (XTH) (Singh et al ., 2011) and expansin (Belfield et al ., 2005; Sane et al ., 2007). However, the precise regulation of these cell wall remodeling genes related to abscission is not yet clear.…”

Section: Introductionmentioning

confidence: 99%

LcEIL2/3 are involved in fruitlet abscission via activating genes related to ethylene biosynthesis and cell wall remodeling in litchi

Yao

et al. 2020

The Plant Journal

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Summary Fruit crops are subject to precocious fruit abscission, during which the phytohormone ethylene (ET) acts as a major positive regulator. However, the molecular basis of ET‐induced fruit abscission remains poorly understood. Here, we show that two ETHYLENE INSENSITIVE 3‐like (EIL) homologs in litchi, LcEIL2 and LcEIL3, play a role in ET‐activated fruitlet abscission. LcEIL2/3 were significantly upregulated in the fruit abscission zone (AZ) during the ET‐induced fruitlet abscission in litchi. The presence of LcEIL2/3 in wild‐type Arabidopsis and ein3 eil1 mutants can accelerate the floral organ abscission. Moreover, the electrophoretic mobility shift assay and dual luciferase reporter analysis illustrated that LcEIL2/3 directly interacted with the gene promoters to activate the expression of cell wall remodeling genes LcCEL2/8 and LcPG1/2, and ET biosynthetic genes LcACS1/4/7 and LcACO2/3. Furthermore, we showed that LcPG1/2 were expressed in the floral abscission zone of Arabidopsis, and constitutive expression of LcPG2 in Arabidopsis promoted the floral organ abscission. In conclusion, we propose that LcEIL2/3 are involved in ET‐induced fruitlet abscission via controlling expression of genes related to ET biosynthesis and cell wall remodeling in litchi.

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