Changes in lipid profiles of liver microsomes of rats following intratracheal administration of DDT or endosulfan

Abstract

The effect of intratracheal administration of DDT (5 mg/100 g body weight) or endosulfan (1 mg/100 g body weight) to rats for three consecutive days, has been studied on liver lipid metabolism. The administration of DDT but not endosulfan significantly increased the liver weight and the microsomal protein contents. Both DDT and endosulfan treatments significantly increased the contents of microsomal phosphatidylcholine (PC), total-free- and esterified cholesterol. The distribution of unsaturated fatty acids of microsomal PC and PE was increased by DDT treatment. The intratracheal administration of DDT caused fatty infiltration of liver which was probably due to increased synthesis of triglycerides (TG). This is supported by the increased incorporation of radioactive palmitate-1-14C into microsomal TG. However, the increased incorporation of palmitate-1-14C into microsomal PC and phosphatidylethanolamine (PE) after the DDT treatment, was due to the increased transacylation reaction supported by the decreased activity of microsomal phospholipase A. The intratracheal administration of endosulfan did not have pronounced effect on liver fatty infiltration, or transacylation reaction in microsomal PC and PE. However, the results have shown that the treatments of DDT or endosulfan increased the PC contents and the incorporation of radioactive [methyl-3H]choline into PC of microsomes, resulting the increased synthesis of PC via CDPcholine pathway. Thus, the intratracheally administered DDT or endosulfan to rats showed that both the insecticides cause manifestations in the biochemistry of microsomal membrane lipids, although the effects of DDT being more pronounced. Therefore, the translocation effects of these insecticides or metabolites from lung to liver is established.

Clinical Aspects of the Poisoning by the Pesticide Endosulfan

“…The induction of phosphatidylcholine (PC) biosynthesis in lung and liver of rats has been shown following the intratracheal administration of endosulfan (1 mg 100 g -1 body weight) for 3 days. 52 As pointed by Narayan et al, 53 biochemical effects of endosulfan are different in many cases from other organochlorine insecticide. For example, DDT and endosulfan have similar effects on microsomal lipid metabolism, but produce different biochemical manifestations on the secretion of surfactant phospholipids.…”

Effects of Dietarymyo-Inositol,D-chiro-Inositol andL-chiro-Inositol on Hepatic Lipids with Reference to the Hepaticmyo-Inositol Status in Rats Fed on 1,1,1-Trichloro-2,2-bis (p-chlorophenyl) Ethane

“…Narayan et al have suggested that feeding DDT to rats increased the hepatic phospholipid due to the enhanced synthesis of PC. 10) The administration of such xenobiotics as DDT, PCB and BHT has elevated hepatic lipids and the activity of drug-metabolizing enzymes. 3,10,11) The induction of drug-metabolizing enzymes by xenobiotics is accompanied by the simultaneous induction of PC synthesis.…”

“…10) The administration of such xenobiotics as DDT, PCB and BHT has elevated hepatic lipids and the activity of drug-metabolizing enzymes. 3,10,11) The induction of drug-metabolizing enzymes by xenobiotics is accompanied by the simultaneous induction of PC synthesis. 10,12) The present study has demonstrated that the hepatic phospholipid level and the proportion of PC were higher in all the DDT-fed groups than in the basal diet-fed group.…”

“…3,10,11) The induction of drug-metabolizing enzymes by xenobiotics is accompanied by the simultaneous induction of PC synthesis. 10,12) The present study has demonstrated that the hepatic phospholipid level and the proportion of PC were higher in all the DDT-fed groups than in the basal diet-fed group. It is thus postulated that exposing rats to several xenobiotics might result in reducing the PI/PC ratio due to not affecting the hepatic free MI level and the incorporation of MI to PI.…”

Hepatic Effects from Subacute Exposure to Insecticides in Adult Male Wistar Rats

“…Studies document OC exposures in Comarca Lagunera, Mexico City, Puebla, Veracruz (RAPAM, 1997) and the Yaqui Valley in Sonora (Guillette et al, 1998). Such exposures are of great concern, especially since exposure to insecticides has been shown to alter lipid metabolism and produce liver damage in rats (Shakoori et al, 1998;Narayan et al, 1990;Videira et al, 2001;Aldana et al, 1998;Aldana et al, 2001). The objective of our study was to evaluate the potential hepatotoxic effects of insecticide exposure at these levels in rats, using serum biochemical indicators of hepatic damage (transaminases, cholesterol, triglycerides, total protein, and albumin), as well as light and electron microscopy to evaluate microstructure hepatic changes.…”